Cytokines in inflammatory bowel disease

Moldoveanu, Al.C.; Diculescu, Mircea; Braticevici, Carmen Fierbinţeanu

doi:10.1515/rjim-2015-0016

Cited by 41 publications

(49 citation statements)

References 71 publications

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“…Plumericin (3 mg/kg, i.p.) 39 Levels of proinflammatory enzymes such as iNOS or COX-2 are also elevated in IBD as a result of the intestinal inflammation. Data are expressed as the mean ± SEM of 10 mice for each group.…”

Section: Discussionmentioning

confidence: 99%

Plumericin prevents intestinal inflammation and oxidative stress in vitro and in vivo

et al. 2019

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Inflammatory bowel diseases (IBDs) are characterized by an inflammatory and oxidative stress condition in the intestinal tissue. In this study, we evaluated the effect of plumericin, one of the main bioactive components of Himatanthus sucuuba (Woodson) bark, on intestinal inflammation and oxidative stress, both in vitro and in vivo. The effect of plumericin (0.5‐2 µM) in vitro was evaluated in rat intestinal epithelial cells (IEC‐6) treated with lipopolysaccharides from E. coli (10 μg/mL) plus interferon‐γ (10 U/mL). Moreover, a 2,4,6‐dinitrobenzene sulfonic acid (DNBS)‐induced colitis model was used to evaluate the anti‐inflammatory and antioxidant activity of plumericin (3 mg/kg) in vivo. The results showed that plumericin significantly reduces intestinal inflammatory factors such as tumor necrosis factor‐α, cyclooxygenase‐2 and inducible nitric oxide synthase expression, and nitrotyrosine formation. Plumericin also inhibited nuclear factor‐κB translocation, reactive oxygen species (ROS) release, and inflammasome activation. Moreover, plumericin activated the nuclear factor erythroid‐derived 2 pathway in IEC‐6. Using the DNBS‐induced colitis model, a significant reduction in the weight loss and in the development of the macroscopic and histologic signs of colon injury, together with a reduced inflammatory and oxidative stress state, were observed in plumericin‐treated mice. These results indicate that plumericin exerts a strong anti‐inflammatory and antioxidant activity. Thus, it might be a candidate for the development of a new pharmacologic approach for IBDs treatment.

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Section: Discussionmentioning

confidence: 99%

Plumericin prevents intestinal inflammation and oxidative stress in vitro and in vivo

et al. 2019

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“…The IL-1 family is expressed throughout the GI tract where it has been associated with autoimmune and chronic inflammatory diseases [68,69]. The IL-1 family of cytokines are predominately produced by stimulated macrophages, monocytes, fibroblasts, and dendritic cells, but can also be produced by lymphocytes and epithelial cells [70,71]. In the gut, various family members including IL-1α, IL-1β, IL-18, IL-33 and IL-37 are produced directly by IECs [72].…”

Section: Il-1 -Driver Of Inflammationmentioning

confidence: 99%

“…In the gut, various family members including IL-1α, IL-1β, IL-18, IL-33 and IL-37 are produced directly by IECs [72]. IL-1α and IL-1β bind to two IL-1 receptor types; IL-1 receptor type I and IL-1 receptor type II [71,73]. Signaling is regulated by the IL-1 receptor antagonist (IL-1Ra), which competitively binds the IL-1RI.…”

Section: Il-1 -Driver Of Inflammationmentioning

confidence: 99%

The role of barrier function, autophagy, and cytokines in maintaining intestinal homeostasis

Elshaer

Begun

2017

Seminars in Cell & Developmental Biology

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Please cite this article as: Elshaer Dana, Begun Jakob.The role of barrier function, autophagy, and cytokines in maintaining intestinal homeostasis.Seminars in Cell and Developmental Biology http://dx.doi.org/10. 1016/j.semcdb.2016.08.018 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Abstract:Intestinal homeostasis is maintained through the interplay of the intestinal mucosa, local and systemic immune factors, and the microbial content of the gut. The cellular processes of autophagy, endoplasmic reticulum stress, the unfolded protein response and regulation of reactive oxygen species production are required to maintain a balance between proinflammatory responses against potential pathogens and a tolerogenic response towards commensal bacteria. Intestinally active cytokines regulate innate immune pathways and cellular pathways within the gut mucosa. Disruption of these processes, or alterations in the cytokine milieu, can result in an improper response to the commensal gut microbial community leading to inappropriate inflammation characteristic of conditions such as inflammatory bowel disease.

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“…It has been previously reported that inflammatory cytokines are mainly derived from immune cells such as monocyte/macrophages and dendritic cells (28) and miR-155 regulates inflammatory cytokine production in tumor-associated macrophages (29). To determine whether miR-155 expression is involved in the secretion of inflammatory cytokines in macrophages, we detected the mRnA of IL-1β and TNF-α and their protein levels in supernatant by RT-PCR and ELISA respectively.…”

Section: S100a8-induced Upregulation Of Mir-155 Enhances the Secretiomentioning

confidence: 99%

S100A8 facilitates the migration of colorectal cancer cells through regulating macrophages in the inflammatory microenvironment

Zha

Sun

et al. 2016

Oncology Reports

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Abstract.Previous studies have shown that S100 calcium-binding protein A8 (S100A8) contributes to the survival and migration of colorectal cancer (CRC) cells. However, whether S100A8 participates in the progression and metastasis of CRC via the regulation of macrophages in the tumor inflammatory microenvironment remains unknown. In this study, phorbol myristate acetate (PMA) was used to induce the differentiation of THP-1 monocytes to macrophages. MTT assay, western blot analysis, immunofluorescence staining, semi-quantitative RT-PCR (semi-PCR), quantitative real-time PCR (qPCR), Gaussia luciferase activity assay and ELISA were performed to analyze the roles and molecular mechanisms of S100A8 in the modulation of macrophages. MTT assay, flow cytometric analysis, Hoechst staining, wound healing and Transwell migration assay were used to test the effect of S100A8 on the viability and migration of CRC cells co-cultured with macrophages in the inflammatory microenvironment. We found that THP-1 monocytes were induced by PMA and differentiated to macrophages. S100A8 activated the NF-κB pathway in the macrophages and promoted the expression of miR-155 and inflammatory cytokines IL-1β and TNF-α in the inflammatory microenvironment mimicked by lipopolysaccharides (LPS). Furthermore, S100A8 contributed to augment the migration but not the viability of the CRC cells co-cultured with the macrophages in the inflammatory microenvironment. Altogether, our study demonstrated that S100A8 facilitated the migration of CRC cells in the inflammatory microenvironment, and the underlying molecular mechanisms may be partially attributed to the overexpression of miR-155, IL-1β and TNF-α through activation of the NF-κB pathway in macrophages. IntroductionColorectal cancer (CRC), for which distant metastasis accounts for the leading cause of cancer mortality, is one of the most malignant gastrointestinal carcinomas worldwide (1). The pathogenesis of CRC is a complex process and involves environmental influences, genetic alterations, and the host immune system and their interactions. The formation of an inflammatory microenvironment also plays a pivotal role in the development of CRC (2). The host microenvironment is comprised of numerous infiltrating immune cell types and resident tumor cells. Among the immune cells, macrophages play an indispensable role. For instance, macrophage infiltration in colon tissue has been observed in both inflammatory bowel disease (IBD) and CRC (4), and the cytokines secreted by macrophages under certain conditions have directly or indirectly stimulated inflammation and tumor progression (5,6). Although great progress has been made, the interactions and the molecular mechanisms between CRC and the inflammatory microenvironment remain unknown. S100A8 (calgranulin A or S100 calcium-binding protein A8) is a member of the low-molecular calcium binding S100 protein family. It also belongs to the family of damage-associated molecular patterns (DAMPs). Studies have shown that S100A8 plays an important role in regul...

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Cytokines in inflammatory bowel disease

Cited by 41 publications

References 71 publications

Plumericin prevents intestinal inflammation and oxidative stress in vitro and in vivo

Plumericin prevents intestinal inflammation and oxidative stress in vitro and in vivo

The role of barrier function, autophagy, and cytokines in maintaining intestinal homeostasis

S100A8 facilitates the migration of colorectal cancer cells through regulating macrophages in the inflammatory microenvironment

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