Cytokines and exosomal miRNAs in skeletal muscle–adipose crosstalk

Guo, Liu; Quan, Menchus; Pang, Weijun; Yin, Yulong; Li, Fengna

doi:10.1016/j.tem.2023.07.006

Cited by 7 publications

(3 citation statements)

References 130 publications

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“…Improving meat quality through exosome-mediated muscle-fat tissue crosstalk is important [ 19 ]. In this study, we explored the reason why the IMF content of SOL was greater than that of EDL.…”

Section: Discussionmentioning

confidence: 99%

“…However, there is no information on the correlation between IMF traits and fast/slow muscle secreted exosomes. Due to the close anatomical location between myofibers and IMFs, exosomes may mediate cell-to-cell communication between these structures [ 18 , 19 ]. To date, the effect of porcine fast/slow muscle exosomes on IMF deposition remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes

Zhao,

Tian,

et al. 2024

J Animal Sci Biotechnol

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Background Pork quality is affected by the type of muscle fibers, which is closely related to meat color, tenderness and juiciness. Exosomes are tiny vesicles with a diameter of approximately 30–150 nm that are secreted by cells and taken up by recipient cells to mediate communication. Exosome-mediated muscle-fat tissue crosstalk is a newly discovered mechanism that may have an important effect on intramuscular fat deposition and with that on meat quality. Various of adipose tissue-derived exosomes have been discovered and identified, but the identification and function of muscle exosomes, especially porcine fast/slow myotube exosomes, remain unclear. Here, we first isolated and identified exosomes secreted from porcine extensor digitorum longus (EDL) and soleus (SOL), which represent fast and slow muscle, respectively, and further explored their effects on lipid accumulation in longissimus dorsi adipocytes. Results Porcine SOL-derived exosomes (SOL-EXO) and EDL-derived exosomes (EDL-EXO) were first identified and their average particle sizes were approximately 84 nm with double-membrane disc- shapes as observed via transmission electron microscopy and scanning electron microscopy. Moreover, the intramuscular fat content of the SOL was greater than that of the EDL at 180 days of age, because SOL intramuscular adipocytes had a stronger lipid-accumulating capacity than those of the EDL. Raman spectral analysis revealed that SOL-EXO protein content was much greater than that of EDL-EXO. Proteomic sequencing identified 72 proteins that were significantly differentially expressed between SOL-EXO and EDL-EXO, 31 of which were downregulated and 41 of which were upregulated in SOL-EXO. Conclusions Our findings suggest that muscle-fat tissue interactions occur partly via SOL-EXO promoting adipogenic activity of intramuscular adipocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes

Zhao,

Tian,

et al. 2024

J Animal Sci Biotechnol

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“…One of the mechanisms by which exercise mediates its beneficial effects consists of a systemic crosstalk mediated EVs containing different molecules, including miRNAs 27 28 29 . However, it remains unresolved the secretory role of skeletal muscle in the response to training and which miRNAs encapsulated in EVs could be relevant in this context, acting as exerkines 30 .…”

Section: Discussionmentioning

confidence: 99%

miR-29a-3p, a new myokine orchestrating resistance exercise via coordinated metabolic responses

Pinto-Hernandez,

Fernandez-Sanjurjo,

Paget

et al. 2024

Preprint

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It remains unclear whether the adaptive response to different exercise models is mediated by EV miRNAs released from skeletal muscle and their functional metabolic role. We sequenced miRNA-loaded plasma EVs obtained from resting mice after 4-weeks endurance or resistance training. Resistance exercise increased the expression of a 11-miRNA profile grouped into two functional clusters. Using both genetically modified animal models and in vitro approaches, we have identified miR-29a-3p as a novel myokine secreted into the bloodstream as EV cargo by contracting skeletal muscle. It is a cornerstone in the adaptation to resistance training by mediating the coordinated expression and secretion of other miRNAs and affecting muscle mass development and energy metabolism in muscle and liver. Taken together, our study suggests a coordinating and determinant role of miR-29a-3p in the response and adaptation to resistance training, possibly due to its role as a myokine through its regulatory role in energy metabolism.

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