Cytokine Regulation of Chronic Inflammation in Asthma

Barnes, P. J.; Chung, Kian Fan; Adcock, Ian M.

doi:10.1016/b978-012352325-9/50008-3

Cited by 4 publications

(4 citation statements)

References 180 publications

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“…Eosinophils, lymphocytes, mast cells, and macrophage/monocytes play important roles in the pathophysiology of asthma; they are involved in airway inflammation via production of various molecules, such as cytokines, enzymes, and adhesion molecules. 6,7 Many of the effector cells, including mast cells in asthma, produce a variety of cytokines. 8 Cytokines, such as interleukin (IL)-6, tumor necrosis factor (TNF)-␣, and granulocyte-macrophage colony-stimulating factor (GM-CSF), are released in a coordinate network and play an important role in chronic inflammation.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Schisandra Fructus Water Extract on Cytokine Release from a Human Mast Cell Line

Kang

Chae

Choi

et al. 2006

Journal of Medicinal Food

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Schisandra fructus has been used for treatment of cough and thirst in Korea. However, its therapeutic mechanisms remain largely unclear. To investigate the biological effect of Schisandra fructus water extract (SFWE), we examined the effect of SFWE on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced pro-inflammatory cytokine secretion in the human mast cell line HMC-1. HMC-1 cells were stimulated with PMA plus A23187 in the presence or absence of SFWE. Tumor necrosis factor (TNF)-alpha, interleukin 6 (IL-6), and granulocyte-macrophage colony-stimulating factor (GM-CSF) productions were measured by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction. Inhibitory IkappaB/nuclear factor-kappaB (NF-kappaB) expression was assessed by western blot. SFWE suppressed PMA plus A23187-induced TNF-alpha, IL-6, and GM-CSF production in dose-dependent manners. Furthermore, SFWE inhibited IkappaB degradation and NF-kappaB nuclear translocation. These results suggest that SFWE inhibits the secretion of pro-inflammatory cytokines in HMC-1 cells through blockade of IkappaB degradation and NF-kappaB activation. Taken together, these findings may help elucidate the mechanism of action of this medicine in the modulation of mast cell activation in inflammatory conditions.

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Section: Introductionmentioning

confidence: 99%

Effects of the Schisandra Fructus Water Extract on Cytokine Release from a Human Mast Cell Line

Kang

Chae

Choi

et al. 2006

Journal of Medicinal Food

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“…There is increasing evidence that a range of pro-inflammatory cytokines play an important role in orchestrating and perpetuating the airway inflammatory response in asthma (3). Among these pro-inflammatory cytokines, interleukin-1 ␤ (IL-1 ␤ ) which is an important mediator controlling inflammatory and immune responses (4,5) has been implicated in asthma.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of impaired beta-adrenoceptor-induced airway relaxation by interleukin-1beta in vivo in the rat.

Kojima

Mak²,

Haddad³

et al. 1996

J. Clin. Invest.

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We studied the in vivo mechanism of ␤ -adrenergic receptor ( ␤ -AR) hyporesponsiveness induced by intratracheal instillation of interleukin-1 ␤ (IL-1 ␤ , 500 U) in Brown-Norway rats. Tracheal and bronchial smooth muscle responses were measured under isometric conditions ex vivo. Contractile responses to electrical field stimulation and to carbachol were not altered, but maximal relaxation induced by isoproterenol (10 Ϫ 6 -10 Ϫ 5 M) was significantly reduced 24 h after IL-1 ␤ treatment in tracheal tissues and to a lesser extent, in the main bronchi. Radioligand binding using [

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“…Asthma is characterized by chronic airway inflammation, with infiltration of eosinophils, lymphocytes, and monocytes/macrophages, and is associated with the increased expression of several inflammatory proteins, including cytokines, enzymes, and adhesion molecules in the airways (1,2). The molecular regulatory pathways involved in the induction of chronic cytokine expression and the recruitment and activation of inflammatory cells in asthma are not well understood, but there is an increasing recognition that these processes involve increased transcription of inflammatory genes, and that this is regulated by transcription factors (3).…”

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confidence: 99%

Activation and Localization of Transcription Factor, Nuclear Factor- κ B, in Asthma

Adcock

et al. 1998

Am J Respir Crit Care Med

410

239

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Asthma is associated with increased expression of inflammatory proteins including cytokines, enzymes, and adhesion molecules. Induction of many of the genes for these proteins is regulated by the transcription factor, nuclear factor-kappaB (NF-kappaB). We therefore examined whether airway cells from patients with asthma show increased activation of NF-kappaB. Nuclear proteins were extracted from cells of induced sputum and from bronchial biopsies of normal subjects and patients with asthma. NF-kappaB-binding to its consensus DNA binding site, as investigated with 32P-labeled oligonucleotides and electrophoretic-mobility-shift assay, showed a 2.5-fold increase (p < 0.003) in NF-kappaB-DNA binding in induced sputum of asthma patients. Nuclear staining, representing activated NF-kappaB, was observed in macrophages of induced sputum. Immunohistochemical examination of bronchial biopsy specimens with an antibody to p65, a constituent of NF-kappaB, showed more airway epithelial cells with nuclear staining in asthma patients (45.1 +/- 7.2% versus 20.7 +/- 3.9%; n = 9; p < 0.01), and a 2.5-fold greater number of cells with cytoplasmic staining in the mucosal region (p < 0.05). Pooled nuclear extracts of bronchial biopsy specimens from asthma patients showed a 44% greater level of NF-kappaB-DNA binding. Activation of NF-kappaB may be the basis for increased expression of many inflammatory genes and for the perpetuation of chronic airway inflammation in asthma.

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Cytokine Regulation of Chronic Inflammation in Asthma

Cited by 4 publications

References 180 publications

Effects of the Schisandra Fructus Water Extract on Cytokine Release from a Human Mast Cell Line

Effects of the Schisandra Fructus Water Extract on Cytokine Release from a Human Mast Cell Line

Mechanisms of impaired beta-adrenoceptor-induced airway relaxation by interleukin-1beta in vivo in the rat.

Activation and Localization of Transcription Factor, Nuclear Factor- κ B, in Asthma

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