Cytokine profiles in chickens infected with virulent and avirulent Marek's disease viruses: Interferon‐gamma is a key factor in the protection of Marek's disease by vaccination

Kano, Rika; Konnai, Satoru; Onuma, Misao; Ohashi, Kazuhiko

doi:10.1111/j.1348-0421.2009.00109.x

Cited by 30 publications

(17 citation statements)

References 42 publications

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“…In bursa a significant difference between HVT and Rispens groups was observed while both vaccines resulted in IL-10 upregulation in thymus without a significant difference between both vaccinated groups. This late increased IL-10 expression is in agreement with Kano et al, (2009) results. They reported that the expression of IL-10 was more prominent in splenocytes and PBMCs of Rispens vaccinated chicken than those of RB1B infected unvaccinated birds.…”

Section: Discussionsupporting

confidence: 82%

“…Those findings are in contrast to what has been observed during MD infection and in agreement with others findings following vaccination. For instance, Kano et al, (2009), found a decreased expression of those cytokines during the early cytolytic phase of infection with virulent MDV, while they were upregulated in CV1998 vaccinated groups.…”

Section: Discussionmentioning

confidence: 99%

“…In parallel, Abdulcareem et al, (2008) have reported an increased expression of IFN-γ gene in feather tips of CVI988 and HVT vaccinated chickens by 10 days PI (M F Abdul-Careem et al, 2008). Similarly, up-regulation of IFN-γ in Peripheral blood mononuclear cells (PBMCs) and splenocytes following Rispens vaccination has been observed (Kano et al, 2009). Additionally, Heidari et al, ( 2014), reported an up regulation of IFN-γ in the cecal tonsils of genetically susceptible and resistant chickens, during the early cytolytic phase of infection with bacterial artificial chromosome (BAC) cloned very virulent (vv) strain of MDV; rMd5-BAC.…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines as the secreted products of CTLs are proposed to play key roles against MDV infection, shaping the viral pathogenesis (Xing & Schat, 2000) and may possess protective responses following vaccination (Djeraba et al 2000;Kano et al 2009). In spite of, the fact that MDV was the first oncogenic virus to which vaccines were available (Baigent et al 2006), the virus is continually evolving toward greater virulence impacting those vaccines efficacy.…”

Section: Introductionmentioning

confidence: 99%

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Cytokines Evaluation of Different Mareks Disease Virus Vaccines in Broiler Chickens

Abdelhamid¹,

Torky²,

Khalil³

2017

AJVS

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Key words:Marek's disease vaccines, Pro-inflammatory Cytokines, RT-PCR, Chicken Marek's disease is an immune suppressive disease that threatens poultry industry. It is controlled by vaccination against MDV. However, the post vaccination immune mechanisms remain vague. Therefore, the objective of this study was to examine the transcripts of the cellular immune responses of two commercial vaccines (CVI988 and HVT strains) compared to control non-vaccinated chickens through the analyses of several cytokine responses. The mRNA was extracted from the bursa of Fabricius and thymus, sampled at 7, 14 and 21 days post-immunization (PI) of both vaccinated and control groups. Several pro-inflammatory and anti-inflammatory cytokine gene expressions were analyzed using RT-PCR. Early significant up-regulation of IFN-γ, a pro-inflammatory cytokine was observed. Also, upregulation of other cytokines like IL-1β and IL-6 was observed at different time points along with minimal nonsignificant expression of inducible nitric oxide synthase (iNOS) in both of vaccinated groups. Meanwhile, a late upregulation of the anti-inflammatory cytokine, IL-10 was only noticed by 21 day PI. These findings show that both vaccinal strains mimic the natural infection by inducing early cell-mediated inflammatory responses, while devoid the long-term immune suppressive effect of natural infection. Additionally, vaccination triggers anti-tumor cell-mediated responses as indicated by the late increase of IL-10 expression. Furthermore, the observed cytokine responses following CV1988 strain supports its previously reported efficacy over HVT strain, making the Rispens vaccine more suitable candidate for Egyptian field conditions. Additionally, the early observed up-regulation of IFN-γ cytokine nominates its administration as an adjuvant with the Rispens vaccine; this may provide synergistic immunomodulatory capacities to the available vaccines and better levels of protection.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytokines Evaluation of Different Mareks Disease Virus Vaccines in Broiler Chickens

Abdelhamid¹,

Torky²,

Khalil³

2017

AJVS

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“…Previous study indicated that vaccinating with MDV vaccine could enhance the expression of the IRF-3 gene in chicken during latent period of MDV infection [45]. And the role of interferon chicken response to MDV infection had been proved [24, 46].…”

Section: Discussionmentioning

confidence: 99%

Expression Pattern of Genes of RLR-Mediated Antiviral Pathway in Different-Breed Chicken Response to Marek’s Disease Virus Infection

Feng

Lian

Huang

et al. 2013

BioMed Research International

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It has been known that the chicken's resistance to disease was affected by chicken's genetic background. And RLR-mediated antiviral pathway plays an important role in detection of viral RNA. However, little is known about the interaction of genetic background with RLR-mediated antiviral pathway in chicken against MDV infection. In this study, we adopted economic line-AA broilers and native Erlang mountainous chickens for being infected with MDV. Upon infection with MDV, the expression of MDA-5 was upregulated in two-breed chickens at 4, 7, and 21 d.p.i. It is indicated that MDA-5 might be involved in detecting MDV in chicken. Interestingly, the expression of IRF-3 and IFN-β genes was decreased in spleen and thymus of broilers at 21 d.p.i, but it was upregulated in immune tissues of Erlang mountainous chickens. And the genome load of MDV in spleen of broiler is significantly higher than that in Erlang mountainous chickens. Meanwhile, we observed that the death of broiler mainly also occurred in this phase. Collectively, these present results demonstrated that the expression patters of IRF-3 and IFN-β genes in chicken against MDV infection might be affected by the genetic background which sequently influence the resistance of chicken response to MDV.

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Neoplastic Diseases

2013

Diseases of Poultry

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Cytokine profiles in chickens infected with virulent and avirulent Marek's disease viruses: Interferon‐gamma is a key factor in the protection of Marek's disease by vaccination

Cited by 30 publications

References 42 publications

Cytokines Evaluation of Different Mareks Disease Virus Vaccines in Broiler Chickens

Cytokines Evaluation of Different Mareks Disease Virus Vaccines in Broiler Chickens

Expression Pattern of Genes of RLR-Mediated Antiviral Pathway in Different-Breed Chicken Response to Marek’s Disease Virus Infection

Neoplastic Diseases

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