Cytokine profile of conditioned medium from human tumor cell lines after acute and fractionated doses of gamma radiation and its effect on survival of bystander tumor cells

Desai, Sejal; Kumar, Amit; Laskar, Siddharth; Pandey, Badri N.

doi:10.1016/j.cyto.2012.08.022

Cited by 115 publications

(80 citation statements)

References 36 publications

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“…HT29 secreted pro-infl ammatory cytokines, such as, tumor necrosis factor (TNF) α and interleukins (IL) 1β and IL 6; growth factors such as platelet-derived growth factor AA and transforming growth factors (TGF) α and β; chemokines such as fractalkine, IL-8, monocyte chemoattractant protein-1 and interferon-γ-induced protein 10; pro-angiogenic factors such as IL-15 and vascular endothelial growth factor; and immune-modulatory cytokines such as granulocyte colony-stimulating factor, granulocyte macrophage colony-stimulating factor and IL-3. It was suggested, based on previous reports on biopsy samples, that in vivo, a similar cytokine secretion profi le can be observed (Desai et al 2013 ).…”

Section: Features and Mechanismsmentioning

confidence: 98%

HT29 Cell Line

Martínez-Maqueda

Miralles

Recio

2015

The Impact of Food Bioactives on Health

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The human colon adenocarcinoma cell line HT29, is not only used to study the biology of human colon cancers, but it is receiving special interest in studies focused on food digestion and bioavailability due to the ability to express characteristics of mature intestinal cells. In the differentiated phenotype, they are able to form a monolayer with tight junctions between cells and a typical apical brush border. In addition, these differentiated cells express brush-border-associated hydrolases typical of the small intestine although the enzymatic activity is lower than that found in vivo. Although they represent a valuable model due to their similarities with enterocytes of the small intestine, their limitations and the relevance to the in vivo situation are also considered in this chapter. The application of this cell line to transport studies of drugs and food compounds is illustrated, especially when the effect of the mucus layer is considered or used as co-culture in combination with Caco-2 cells. They have also been frequently used to study the intestinal immune response to bacterial infection, and microorganism survival, adhesion or invasion. Finally, the use of these cells to evaluate the effect of several food compounds and mucin secretion is summarized. Keywords HT29 • Cell differentiation • Transport studies • Intestinal cell OriginThe human colon adenocarcinoma cell line HT29 was isolated from a primary tumor of a 44 years old Caucasian female in 1964 by Fogh and Trempe ( 1975 ). Since then, many cell lines have been derived from human colon cancers. Initially, this cell line was used to study different aspects of the biology of human cancers. However, these cells have attracted attention due to the fact they were able to express characteristics of mature intestinal cells, such as enterocytes or mucus producing cells.

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Section: Features and Mechanismsmentioning

confidence: 98%

HT29 Cell Line

Martínez-Maqueda

Miralles

Recio

2015

The Impact of Food Bioactives on Health

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“…Of importance is that it has been shown that G-CSF is released in mouse and NHP radiation models in a dose-dependent manner in response to ionizing radiation [3,19]. This has been substantiated by Desai et al [20] who found G-CSF (and other cytokines) to be secreted into the conditioned media of five different human tumor cell lines 48 hours after irradiation. Although a full dose-response curve for irradiation was not generated, 6 Gy irradiation evoked a significantly greater release of G-CSF than did 2 Gy.…”

Section: Introductionmentioning

confidence: 52%

Radiation Dose-Rate Effects on Select Biomarkers in a Mouse Total-Body Irradiation Model

Hegge¹,

Kin²

2017

RadJ

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Abstract. In the event of acute radiation exposure, absorbed dose may be unknown and biodosimetry tools are needed by first responders to properly triage patients. We evaluated two protein markers -FMS-related tyrosine kinase 3 ligand (Flt3L) and granulocyte colony-stimulating factor (G-CSF) -that are known to be elevated after an acute radiation exposure, as well as total white blood cell (WBC) count changes pre-and post-irradiation at different dose-rates. Female B6D2F1 mice were divided into one sham-irradiated control group and four total-body irradiated groups. Experimental groups received a total dose of 8 Gy of 60 Co gamma photon irradiation at four dose-rates: 0.04, 0.15, 0.30, and 0.47 Gy min -1 . Blood samples from mice were collected at 24 and 48 hours post-exposure for WBC and protein biomarkers (Flt3L and G-CSF). Flt3L values at all dose-rates except 0.15 were significantly elevated from controls but not each other. The G-CSF levels in mouse groups of 0.47 Gy min -1 and 0.04 Gy min -1 were significantly different from controls, and 0.15 Gy min-1 significantly differed from 0.47 Gy min -1 . WBC changes from baseline showed that all experimental groups were significantly lower than controls, and additionally the 0.04 Gy min -1 group was significantly lower than the 0.30 Gy min -1 group. Though more research is needed, it would appear that at the fixed dose, dose-rates, and time points chosen herein may not be particularly strong or show predictable differences in the selected biomarker expression levels.

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“…E-cadherin and vimentin are markers of epithelial and mesenchymal cells, respectively (6). It has been previously demonstrated in A549 human lung carcinoma cell line that a number of cytokines are associated with EMT, including IL-8, VEGF, TGFβ and TNFα (26). It has also been reported that TGFβ is able to induce EMT in multiple cell lines via activation of the E-cadherin repressor (19).…”

Section: Regulation Of Emtmentioning

confidence: 99%

Epithelial-mesenchymal transition in cancer: Role of the IL-8/IL-8R axis

et al. 2017

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Abstract. Epithelial-mesenchymal transition (EMT) is a biological process that is associated with cancer metastasis and invasion. In cancer, EMT promotes cell motility, invasion and distant metastasis. Interleukin (IL)-8 is highly expressed in tumors and may induce EMT. The IL-8/IL-8R axis has a vital role in EMT in carcinoma, which is regulated by several signaling pathways, including the transforming growth factor β-spleen associated tyrosine kinase/Src-AKT/extracellular signal-regulated kinase, p38/Jun N-terminal kinase-activating transcription factor-2, phosphoinositide 3-kinase/AKT, nuclear factor-κB and Wnt signaling pathways. Blocking the IL-8/IL-8R signaling pathway may be a novel strategy to reduce metastasis and improve patient survival rates. This review will cover IL-8-IL-8R signaling pathway in tumor epithelial-mesenchymal transition.

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Cytokine profile of conditioned medium from human tumor cell lines after acute and fractionated doses of gamma radiation and its effect on survival of bystander tumor cells

Cited by 115 publications

References 36 publications

HT29 Cell Line

HT29 Cell Line

Radiation Dose-Rate Effects on Select Biomarkers in a Mouse Total-Body Irradiation Model

Epithelial-mesenchymal transition in cancer: Role of the IL-8/IL-8R axis

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