Cytokine profile in cervical mucosa of Japanese patients with cervical intraepithelial neoplasia

Iwata, Takashi; Fujii, Takuma; Mitsudo, Kenji; Saito, Masafumi; Sugiyama, Juri; Nishio, Hiroshi; Morisada, Tohru; Tanaka, Kyoko; Yaguchi, Tomonori; Kawakami, Yutaka; Aoki, Daisuke

doi:10.1007/s10147-014-0680-8

Cited by 28 publications

(25 citation statements)

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“…Inflammatory factors play a key role in the development of brain inflammation. Cytokines are a broad category of small proteins released by a large range of cells including chemokines, interferons, interleukins, tumor necrosis factor, growth factor and colony stimulating factor [ 14 ]. It has been shown that the CC type chemokines are involved in mediating eosinophil chemotaxis in various allergic inflammatory responses and autoimmune diseases through interaction with corresponding receptors on the surface of eosinophils [ 15 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection

Wei

et al. 2015

Parasites Vectors

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BackgroundAngiostrongylus cantonensis (A. cantonensis) infection can result in increased risk of eosinophilic meningitis. Accumulation of eosinophils and inflammation can result in the A. cantonensis infection playing an important role in brain tissue injury during this pathological process. However, underlying mechanisms regarding the transcriptomic responses during brain tissue injury caused by A. cantonensis infection are yet to be elucidated. This study is aimed at identifying some genomic and transcriptomic factors influencing the accumulation of eosinophils and inflammation in the mouse brain infected with A. cantonensis.MethodsAn infected mouse model was prepared based on our laboratory experimental process, and then the mouse brain RNA Libraries were constructed for deep Sequencing with Illumina Genome Analyzer. The raw data was processed with a bioinformatics’ pipeline including Refseq genes expression analysis using cufflinks, annotation and classification of RNAs, lncRNA prediction as well as analysis of co-expression network. The analysis of Refseq data provides the measure of the presence and prevalence of transcripts from known and previously unknown genes.ResultsThis study showed that Cys-Cys (CC) type chemokines such as CCL2, CCL8, CCL1, CCL24, CCL11, CCL7, CCL12 and CCL5 were elevated significantly at the late phase of infection. The up-regulation of CCL2 indicated that the worm of A. cantonensis had migrated into the mouse brain at an early infection phase. CCL2 could be induced in the brain injury during migration and CCL2 might play a major role in the neuropathic pain caused by A. cantonensis infection. The up-regulated expression of IL-4, IL-5, IL-10, and IL-13 showed Th2 cell predominance in immunopathological reactions at late infection phase in response to infection by A. cantonensis. These different cytokines can modulate and inhibit each other and function as a network with the specific potential to drive brain eosinophilic inflammation. The increase of ATF-3 expression at 21 dpi suggested the injury of neuronal cells at late phase of infection. 1217 new potential lncRNA were candidates of interest for further research.ConclusionsThese cytokine networks play an important role in the development of central nervous system inflammation caused by A. cantonensis infection.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-0939-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection

Wei

et al. 2015

Parasites Vectors

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“…Some authors attribute the rise in IL-1β plasma to an increased risk of cervical cancer in women with polymorphisms (Qian et al, 2010). Likewise, a greater concentration of IL-1β in the vaginal mucosa was reported in women with high grade cervical lesions (Iwata et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Reduced Expression of IL-1β and IL-18 Proinflammatory Interleukins Increases the Risk of Developing Cervical Cancer

Matamoros

Silva

Moura

et al. 2019

Asian Pac J Cancer Prev

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Background: The objective of this study was to analyze the gene expression profile of the proinflammatory interleukins, (IL-1β and IL-18) in patients with premalignant lesions and cervical cancer. Methods: Total IL-1β and IL-18 mRNA was quantified by qPCR to obtain the expression data in cervical tissues. A total of 74 cervical biopsies were obtained from women undergoing a colposcopy. The samples were divided into: normal (19), low level lesions (LSIL) or NIC I (17), high level lesions (HSIL) or CIN II and CIN III (29) and cancer (9). The normal cervical tissue samples were included as controls. The OR and 95% CI were calculated for the determination of the risk of progression between each type of lesion and cancer using logistic regression. Results: The results showed that an increase in the risk of progression of pre-neoplastic lesions to cancer was between 2.5 and 2.08 times higher in women with lower IL-1β and IL-18 expression, respectively. Conclusions: This study provided evidence that IL-1β and IL-18 are potential biomarkers that can be explored in further studies for monitoring the evolution of pre-neoplastic lesions and avoiding overtreatment or undertreatment of the patients.

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“…Opinions vary -whether the highest IL-8 expression is in HSIL (CIN II-CIN III) (7,23) or in LSIL (CIN I) where there is an active immune response (11,49). However, researchers agree that the expression is higher in CIN than in normal tissues (11,24,44,46). In our study, it was observed that IL-8 expression in squamous epithelium and inflammatory cells decreases trough CIN staging, but its expression in blood vessel endothelium and stroma increases.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence is inconclusive whether the highest expression of IL-8 is found in CIN II-CIN III (7,30) or in CIN I (11). It must be noted that the expression of IL-8 is higher in CIN when compared to normal tissues (7,11,24,30,42). IL-8 secretion and hypoxia of the tissue is a linked process -oxygen deprivation increases IL-8 production (27).…”

Section: Introductionmentioning

confidence: 99%

Interleukin IL-1α, IL-6, IL-8, IL-10 Expression in Different Staging of Cervical Intraepithelial Neoplasia

Balode

Pilmane

Rezeberga

et al. 2017

Acta Chirurgica Latviensis

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Summary Introduction. Cervical cancer is the fourth most common form of cancer in women [19]. The precancerous stages are divided into three distinctive stages, labelled cervical intraepithelial neoplasia (CIN) I, II and III. One of the aetiological factors is chronic inflammation in cervical tissue, most often induced by Human papilloma virus (HPV). 88,5% of the patients regress from low grade intraepithelial changes to unchanged epithelium [14]. It has been proposed that cytokine balance plays a key role in the development of high grade epithelial changes (CIN I – CIN III) in the remaining 11.5% of patients, however, the exact trigger of this event remains to be found. Aim of the Study. The aim of the study was to determine three pro-inflammatory (IL-1α, IL-6, IL-8) and one anti-inflammatory (IL-10) interleukin expression in different CIN cervix uteri biopsies. Material and methods. 16 biopsies were obtained with different CIN staging: one with CIN I stage, five with CIN II stage and 10 with CIN III stage. The samples were stained with haematoxylin and eosin and immunohistochemistry for IL-1α, IL-6, IL-8 and IL-10. Slides were evaluated semi-quantitatively grading the intensity of positively stained structures in the visual field. Results. Examination of the samples yielded the following: IL-1α expression increased from CIN II to CIN III in squamous epithelium, while IL-8 expression decreased. A few IL-1α containing inflammatory cells were found in all CIN stages. IL-8 expression in subepithelium and the number of inflammatory cells decreased from CIN II stage to CIN III, although, it increased in the blood vessel endothelium. Conclusions. There was constant moderate expression of both IL-6 and IL-10 during all CIN stages, except for inflammatory cells, where IL-6 expression was high during all stages, yet there were few IL-10 containing cells during CIN. The balanced expression of both cytokines suggests that pro- and anti-inflammatory cytokine balance has an important role in CIN morfopathogenesis. The high expression of IL-6 in inflammatory cells and constant expression trough CIN staging indicates sustentation of chronic inflammation and production of other cytokines, such as IL-8, IL-1α. The variable IL-8 expression and its decrease in CIN III stage suggests the depletion of IL-8 production. The high expression of cytokines in blood vessel endothelium indicates their important role in CIN morfopathogenesis.

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Cytokine profile in cervical mucosa of Japanese patients with cervical intraepithelial neoplasia

Cited by 28 publications

References 35 publications

Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection

Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection

Reduced Expression of IL-1β and IL-18 Proinflammatory Interleukins Increases the Risk of Developing Cervical Cancer

Interleukin IL-1α, IL-6, IL-8, IL-10 Expression in Different Staging of Cervical Intraepithelial Neoplasia

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