Cytokine gene expression in Walker 256: A comparison of variants A (aggressive) and AR (regressive)

Perroud, Ana Paula De Almeida Salles; Ashimine, Rika; Castro, Gláucia Monteiro de; Guimarães, Fernando; Vieira, Karla Priscila; Vilella, Conceição Aparecida; Cavalcanti, Tereza Cristina Samico; Zollner, Ricardo de Lima

doi:10.1016/j.cyto.2006.11.004

Cited by 5 publications

(1 citation statement)

References 30 publications

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“…Moreover, little attention has been given to the association between the aforementioned antineoplastic effects and its previously observed antioxidant properties (Sandoval et al, 2002;Pilarski et al, 2006). In face of the promising potential of UT for the adjuvant management of neoplastic diseases, this investigation attempted to evaluate the joint antiproliferative and antioxidant activity of an UT hydroalcoholic extract in the Walker-256 (W-256) cancer model, which is a murine carcinosarcoma that has been considered an appropriate model for studies concerned to solid tumors, oncologic pain, metastasis and cancer-related cachexia (De Almeida Salles Perroud et al, 2006;Brigatte et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Dreifuss

Bastos-Pereira

Ávila

et al. 2010

Journal of Ethnopharmacology

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Section: Introductionmentioning

confidence: 99%

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Dreifuss

Bastos-Pereira

Ávila

et al. 2010

Journal of Ethnopharmacology

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Production of cachexia mediators by Walker 256 cells from ascitic tumors

Rebeca

Bracht

Noleto

et al. 2008

Cell Biochemistry & Function

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In neoplasic cachexia, chemical mediators seem to act as initiators or perpetuators of this process. Walker 256 cells, whose metabolic properties have so far been little studied with respect to cancer cachexia, are used as a model for the study of this syndrome. The main objective of this research was to pinpoint the substances secreted by these cells that may contribute to the progression of the cachectic state. Since inflammatory mediators seem to be involved in the manifestation of this syndrome, the in vitro production of nitric oxide (NO), cytokines (tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6)), and prostaglandin E2 (PGE2) was evaluated in Walker 256 cells isolated from ascitic tumors. After 4 or 5 h, a significant increase in NO production was observed (2.55 +/- 1.56 and 4.05 +/- 1.99 nmol NO per 10(7) cells, respectively). When isolated from a 6-day-old tumor, a significantly lower production of IL-6 and higher production of TNF-alpha than in cells from a 4-day-old tumor were observed, indicating a relationship between the production of cytokines and the time of tumor development after implantation. Considerable production of PGE(2) by Walker 256 cells isolated from the 6-day-old tumor was also observed. Polyamines were also determined in Walker 256 cells. Levels of putrescine, spermidine, and spermine did not show significant differences in tumors developed during 4 or 6 days. Direct evidence of the release of proinflammatory cytokines and PGE2 by Walker 256 cells suggests that these mediators can drive the cachectic syndrome in the host, the effect being dependent on tumor development time.

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Ultrastructural and Stereological Analysis of Walker 256 Carcinosarcoma Cells at Various Stages of Their Differentiation

et al. 2009

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Ultrastructural and stereological study of Walker 256 carcinosarcoma cells implanted into the femoral muscle of Wistar rats was performed. Two types of Walker 256 carcinosarcoma cells were distinguished and their ultrastructural analysis was carried out. Five differentiation stages were described for each of the two cell types. Differentiation of carcinosarcoma cells was associated with a decrease of the nucleus/cytoplasm ratio, enlargement of the cell, and hyperplasia of granular cytoplasmic reticulum elements, fixed ribosomes, and mitochondria. Differences in the ultrastructure of the two cell types at similar differentiation stages were detected.

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Cytokine gene expression in Walker 256: A comparison of variants A (aggressive) and AR (regressive)

Cited by 5 publications

References 30 publications

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Production of cachexia mediators by Walker 256 cells from ascitic tumors

Ultrastructural and Stereological Analysis of Walker 256 Carcinosarcoma Cells at Various Stages of Their Differentiation

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