Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies

Hwang, Jong Ha; Lee, Mi Jung; Seok, Oye Sun; Paek, Yu Chin; Cho, Geum Joon; Seol, Hyun Joo; Lee, Jae Kwan; Oh, Min Jeong

doi:10.1016/j.cyto.2009.08.013

Cited by 58 publications

(45 citation statements)

References 33 publications

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“…MSCs, besides, several groups have isolated MSCs from umbilical cord, placenta, and decidua [11][12][13]. Aberrant levels of cytokines were observed in placenta-derived MSCs from PE patients, and higher levels of MSC negative markers were found in the placentas from PE patients [14,15]. These findings suggested that MSCs could contribute to PE pathogenesis.…”

Section: Introductionmentioning

confidence: 80%

MicroRNA‐181a Regulates Local Immune Balance by Inhibiting Proliferation and Immunosuppressive Properties of Mesenchymal Stem Cells

et al. 2012

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Section: Introductionmentioning

confidence: 80%

MicroRNA‐181a Regulates Local Immune Balance by Inhibiting Proliferation and Immunosuppressive Properties of Mesenchymal Stem Cells

et al. 2012

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“…Several previous studies reported that amniotic membranes secreted interleukins [1,3,[7][8][9][10][11], however, the secretion of growth factors was less characterized. Therefore, we measured 41 different growth factors and receptors that were known to be present in other tissues.…”

Section: Discussionmentioning

confidence: 99%

Growth factors and their receptors derived from human amniotic cells in vitro

Grzywocz

Pius-Sadowska

Kłos

et al. 2014

Folia Histochem Cytobiol.

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Abstract:In vitro studies have shown that amnion-produced growth factors participated in angiogenesis, re-epithelialization, and immunomodulation. The aim of our study was to investigate the growth factors and receptors produced by human amnion tissue and amniotic cells. Human amnions (hAM) were isolated, and amnion circles were dissected for in vitro analysis. Some amnion fragments were digested by the use of different methods to obtain two cell fractions, which were analysed for mesenchymal and epithelial cell markers. Amniotic circles and human amniotic cell fractions were cultured in a protein-free medium. Proteins secreted into the culture medium were analysed with a human growth factor antibody array. Conditioned culture media were added to human umbilical vein epithelial cells (HUVECs) to test for stimulation of migration (scratch test) and proliferation (Ki67 expression). Fraction 1 cells expressed both cytokeratin and mesenchymal cell markers which indicated that it was composed of a mixture of human amnion epithelial cells (hAECs) and mesenchymal stromal cells (hAMSCs). Fraction 2 cells mainly expressed cytokeratin and, therefore, were designed as hAECs. Secretion of proteins by the cultured cells increased with time. The hAM cultures secreted EGF-R, IGF, and IGFBP-2, -3 and -6; Cell Fraction 1 secreted NT-4, whereas Cell Fraction 2 secreted G-CSF, M-CSF, and PDGF. Conditioned media of hAM cultures stimulated HUVECs migration. We have showed for the first time that human amnions and amniotic cells secreted IGFBP-6, MCSF-R, PDGF-AB, FGF-6, IGFBP-4, NT-4, and VEGF-R3. We found that Cell Fraction 1, Cell Fraction 2, and the whole amnion secreted different proteins, possibly due to different proportions of amnion-derived cells and different cell-cell interactions. The hAM cell factors remained functional in vitro and induced intensified migration of HUVECs. The growth factors and receptors found in amnion or amniotic cell media might be used for regenerative medicine.

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“…In particular, human amniotic mesenchymal stem cells (hA-MSCs) have been shown to differentiate into cell types of mesenchymal origin such as chondrocytes, adipocytes, and osteocytes [20][21][22]. The phenotype of hAMSCs from normal pregnant women has been characterized and found to differ in terms of cytokine expression from that of pregnant women affected by pre-eclampsia [23].…”

Section: Introductionmentioning

confidence: 99%

High Aminopeptidase N/CD13 Levels Characterize Human Amniotic Mesenchymal Stem Cells and Drive Their Increased Adipogenic Potential in Obese Women

Iaffaldano

Nardelli

Raia

et al. 2013

Stem Cells and Development

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Maternal obesity is associated to increased fetal risk of obesity and other metabolic diseases. Human amniotic mesenchymal stem cells (hA-MSCs) have not been characterized in obese women. The aim of this study was to isolate and compare hA-MSC immunophenotypes from obese (Ob-) and normal weight control (Co-) women, to identify alterations possibly predisposing the fetus to obesity. We enrolled 16 Ob-and 7 Co-women at delivery (mean/SEM prepregnancy body mass index: 40.3/1.8 and 22.4/1.0 kg/m 2 , respectively), and 32 not pregnant women. hA-MSCs were phenotyped by flow cytometry; several maternal and newborn clinical and biochemical parameters were also measured. The expression of membrane antigen CD13 was higher on Ob-hA-MSCs than on Co-hA-MSCs (P < 0.005). Also, serum levels of CD13 at delivery were higher in Ob-versus Co-pregnant women and correlated with CD13 antigen expression on Ob-hA-MSCs (r 2 = 0.84, P < 0.0001). Adipogenesis induction experiments revealed that Ob-hA-MSCs had a higher adipogenic potential than Co-hA-MSCs as witnessed by higher peroxisome proliferator-activated receptor gamma and aP2 mRNA levels (P < 0.05 and P < 0.05, respectively), at postinduction day 14 associated with increased CD13 mRNA levels from baseline to day 4 postinduction (P < 0.05). Adipogenesis was similar in the two sets of hA-MSCs after CD13 silencing, whereas it was increased in Co-hA-MSCs after CD13 overexpression. CD13 expression was high also in Ob-h-MSCs from umbilical cords or visceral adipose tissue of not pregnant women. In conclusion, antigen CD13, by influencing the adipogenic potential of hA-MSCs, could be an in utero risk factor for obesity. Our data strengthen the hypothesis that high levels of serum and MSC CD13 are obesity markers.

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Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies

Cited by 58 publications

References 33 publications

MicroRNA‐181a Regulates Local Immune Balance by Inhibiting Proliferation and Immunosuppressive Properties of Mesenchymal Stem Cells

MicroRNA‐181a Regulates Local Immune Balance by Inhibiting Proliferation and Immunosuppressive Properties of Mesenchymal Stem Cells

Growth factors and their receptors derived from human amniotic cells in vitro

High Aminopeptidase N/CD13 Levels Characterize Human Amniotic Mesenchymal Stem Cells and Drive Their Increased Adipogenic Potential in Obese Women

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