Cytokine Expression in Patients Hospitalized for Severe Odontogenic Infection in Brazil

Sette-Dias, Augusto César; Maciel, Kamilla Faria; Abdo, Evandro Neves; Brito, Luciana Carla Neves de; Carvalho, Maria Auxiliadora Roque de; Vieira, Leda Quércia; Farias, Luiz M.; Sobrinho, Antônio Paulino Ribeiro; Magalhães, Paula Prazeres

doi:10.1016/j.joen.2016.01.018

Cited by 9 publications

(11 citation statements)

References 32 publications

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“…It has been demonstrated previously that caries-exposed pulps show significantly higher levels of IL-8 compared to normal teeth (7). IL-8 levels were also found to be increased among patients with severe odontogenic infections, while immunoregulators were suppressed during this period (19). IL-8 levels were also found to be increased among patients with severe odontogenic infections, while immunoregulators were suppressed during this period (19).…”

Section: Discussionmentioning

confidence: 61%

Effect of root canal debridement on inflammatory cytokine levels

et al. 2018

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In endodontic infections, inflammatory mediators such as cytokines are released, recruited and retained until the infection is eradicated. Root canal therapy is performed to prevent the spread of infection. The aim of this study was to investigate the effects of root canal debridement (cleaning and shaping) on periapical inflammation by measuring the levels of inflammatory cytokines, Interleukin-8 (IL-8) and Interleukin-10 (IL-10). The study includes twenty patients with pulp necrosis and asymptomatic apical periodontitis. Periradicular sample was collected using paper points before and after root canal debridement. Cytokine levels were determined by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). Data were analysed using paired t-test (PASW Statistics 18) (P = 0.05). All samples showed the presence of IL-8 and IL-10 prior to root canal debridement. Significantly reduced levels (P < 0.05) of IL-8 and IL-10 were detected after root canal debridement. In conclusion, root canal debridement significantly decreased the levels of the tested pro- and anti-inflammatory cytokine in the periradicular interstitial fluid.

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Section: Discussionmentioning

confidence: 61%

Effect of root canal debridement on inflammatory cytokine levels

et al. 2018

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“…, , Sette‐Dias et al . ). If PTF volume was measured, only a wetted length (mm): volume (μL) calibration curve was used to determine this.…”

Section: Resultsmentioning

confidence: 97%

“…()Dentsply‐Mai.152 mm past WL601After Instrumenting–ELISA––––Sette‐Dias et al . ()––2 mm past WL602After Instrumenting14 daysqPCRTRIzol (−µL)10–25 at 12 000 g Zhi et al . ()–30WL302After Instrumenting7 daysCBA–––30 at 4000 g Filter Paper StripKuo et al .…”

Section: Resultsmentioning

confidence: 99%

“…Four studies used 1 point per sample(Wahlgren et al 2002, Liu et al 2003, Pezelj-Ribari c et al 2007, Shahriari et al 2011 whereas five used 2(Atao glu et al 2002, Alptekin et al 2005a,b, Ehsani et al 2012, Grga et al 2013, eleven used 3(Henriques et al 2011, Tavares et al 2012, Rechenberg et al 2014, Bambirra et al 2015, Martinho et al 2015, Sette-Dias et al 2016, four used ≤ 5…”

mentioning

confidence: 99%

“…or a fixed 3 -4 mm(Shahriari et al 2011, Tavares et al 2012, Bambirra et al 2015, Martinho et al 2015, Sette-Dias et al 2016.…”

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confidence: 99%

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A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

et al. 2019

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Aim The primary aim was to identify techniques used to sample and analyse periradicular tissue fluid (PTF) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach. Methodology Data Sources: PubMed, EMBASE, Cochrane Library, Science Direct, Web of Science and OpenGrey. Eligibility Criteria: Clinical studies published until 1 June 2018 which utilized orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded. Study appraisal and methods: In accordance with PRISMA guidelines, data were extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted, and studies were critically appraised using a modified version of the Cochrane risk of bias tool. Results Study Characteristics: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points (n = 27), fine needle aspiration (n = 4) and filter strips (n = 2). Assay techniques included enzyme‐linked immunosorbent assay (n = 18), quantitative polymerase chain reaction (n = 9), radioimmunoassay (n = 4), colorimetric assay (n = 2), immunofluorometric assay (n = 1) and cytometric bead array (n = 1). Forty‐five different inflammatory mediators were targeted at the proteomic/metabolomic (n = 25) or transcriptomic level (n = 9). Limitations: Significant heterogeneity exists within the methodology, and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols. Conclusions Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during root canal treatment. The most studied analytes were IL‐1β and TNF‐α. Implications: Further research is required to develop an optimized PTF sampling and eluting protocol to overcome methodological heterogeneity, and future studies are advised to follow a standardized approach to reporting their methodology.

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