Cytokine expression and Mycoplasma hyopneumoniae burden in the development of lung lesions in experimentally inoculated pigs

Almeida, H. M. S.; Mechler-Dreibi, Marina Lopes; Sonálio, Karina; Ferraz, Maria Eugênia Silveira; Storino, Gabriel Yuri; Barbosa, Fernanda de Oliveira; Maes, Dominiek; Montassier, Hélio José; Oliveira, Luís Guilherme de

doi:10.1016/j.vetmic.2020.108647

Cited by 24 publications

(31 citation statements)

References 42 publications

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“…Although mycoplasma pneumonia of swine (MPS) is a serious threat to pig production, the mechanism by which the inflammatory response to M. hyopneumoniae is regulated is rarely studied. It was reported that M. hyopneumoniae infection could cause inflammation in live pigs [22] and induce the secretion of IL-6 and IL-8 from PAM 3D4/21 cells [23], which is consistent with our research. In our study, CYP3A29 and PXR were upregulated after M. hyopneumoniae infection in PAM 3D4/21 cells.…”

Section: Discussionsupporting

confidence: 93%

Effect of Pregnane X Receptor on CYP3A29 Expression in Porcine Alveolar Macrophages during Mycoplasma hyopneumoniae Infection

Yang

Xing

Wang

et al. 2021

Animals

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Mycoplasma hyopneumoniae (M. hyopneumoniae, Mhp) is the causative agent of mycoplasma pneumonia of swine (MPS). M. hyopneumoniae infection causes inflammation in pigs and leads to considerable economic losses in the pig industry. Pregnane X receptor (PXR) is a pluripotent gene regulatory protein that plays an important role in regulating cytochrome P-450 (CYP) in pigs in the context of inflammatory responses, drug metabolism, homeostasis, etc. We previously reported that cytochrome P450 3A29 (CYP3A29) expression was significantly upregulated in pigs infected with M. hyopneumoniae compared with healthy control pigs. This experiment mainly focused on identifying the role of PXR in the regulation of CYP3A29 and inflammatory factors after M. hyopneumoniae infection by establishing pig alveolar macrophage (PAM) cells in which PXR was overexpressed or silenced. Our results showed that the overexpression of PXR could significantly improve the protein and the mRNA expression levels of CYP3A29 with and without M. hyopneumoniae infection in PAM cells. After the expression of PXR was inhibited, protein and mRNA expression levels of CYP3A29 were significantly reduced with and without M. hyopneumoniae infection in PAM cells. Moreover, PXR can regulate the mRNA expression levels of IL-6 and IL-8 during M. hyopneumoniae infection of PAM cells. In conclusion, these results suggest that PXR positively regulates CYP3A29 expression during the inflammatory response caused by M. hyopneumoniae infection.

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Section: Discussionsupporting

confidence: 93%

Effect of Pregnane X Receptor on CYP3A29 Expression in Porcine Alveolar Macrophages during Mycoplasma hyopneumoniae Infection

Yang

Xing

Wang

et al. 2021

Animals

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“…These data suggest that only Mhyo can worsen MLCL, and that Mhr and Mfloc are apparently not Mhyo's opportunistic agents. Almeida et al (2020), in an experimental study, stated that the MLCL showed a regressive trend as Mhyo infection evolved.…”

Section: Discussionmentioning

confidence: 99%

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

Ferreira

Mechler-Dreibi

Sonálio

et al. 2021

Veterinary Microbiology

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“…Semiquantitative analysis of microscopic lesions associated with Mh has only been applied in experimental Mh infection [ 22 , 30 , 31 ]. In these fattening pigs with natural infection, chronic lesions (III and IV) were observed more often (31.7% and 43.9%) than early lesions (I and II) (9.8% and 14.6%), which is in agreement with the chronicity and lesion progression described in experimental studies [ 11 , 22 , 30 , 31 ]. Grade III and IV lesions were detected primarily in animals with CBP that remained on the farm for a longer period (209.1 and 215.8 days of age, respectively).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Lung Disease in Finishing Pigs at Slaughter: Pulmonary Lesions and Implications on Productivity Parameters

Paz-Sánchez

Herráez

Quesada‐Canales

et al. 2021

Animals

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Swine respiratory disease is associated with productive losses. We evaluated the prevalence of lung lesions with an emphasis on Mycoplasma hyopneumoniae (Mh), porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), as well as the impact on productive parameters in 108 finishing pigs at slaughter. Pathologic, immunohistochemical (IHC) and serologic analyses were performed. Pneumonic processes were observed in 73.1% of the animals. They mainly consisted of cranioventral bronchopneumonia (CBP) (46.3%) and pleuritis (17.6%). Microscopically, bronchointerstitial pneumonia (67.4%) was common and was occasionally combined (27.9%) with interstitial pneumonia (IP). Mh and PCV2-antigens were detected in bronchointerstitial pneumonia (70.7%) and IP cases (33.3%). There were low titers against Mh (18%) and high titers against PRRSV (100%) and PCV2 (65%). Animals with CBP remained at the farm longer; those with >10% of lung parenchyma involvement were sent later (208.8 days old) and had a lower average carcass weight (74.1 kg) and a lower daily weight gain (500.8 gr/day) compared with animals without lesions (567.2 gr/day, 77.7 kg, 200.8 days old). We suggest that animals that do not reach the weight at slaughter should be sent to slaughter regardless to avoid further negative impacts of respiratory disease in productive parameters.

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Cytokine expression and Mycoplasma hyopneumoniae burden in the development of lung lesions in experimentally inoculated pigs

Cited by 24 publications

References 42 publications

Effect of Pregnane X Receptor on CYP3A29 Expression in Porcine Alveolar Macrophages during Mycoplasma hyopneumoniae Infection

Effect of Pregnane X Receptor on CYP3A29 Expression in Porcine Alveolar Macrophages during Mycoplasma hyopneumoniae Infection

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

Assessment of Lung Disease in Finishing Pigs at Slaughter: Pulmonary Lesions and Implications on Productivity Parameters

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