Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

Ferreira, M. M.; Mechler-Dreibi, Marina Lopes; Sonálio, Karina; Almeida, Henrique Meiroz de Souza; Ferraz, Maria Eugênia Silveira; Jacintho, Ana Paula Prudente; Maes, Dominiek; Oliveira, Luís Guilherme de

doi:10.1016/j.vetmic.2021.109123

Cited by 8 publications

(15 citation statements)

References 32 publications

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“…The qPCR results showed a high occurrence of M. hyopneumoniae in H2 and H3 and high correlation with MLCL, which was also recently reported by Ferreira et al [ 5 ]. In addition, the authors indicated that the greater the extent of MLCL, the greater the concentration of M. hyopneumoniae and, interestingly, the lower the concentration of M. flocculare in the lungs.…”

Section: Discussionsupporting

confidence: 86%

“…Three commercial swine herds (H), located in the state of São Paulo—Brazil, were selected for the study: one herd was free of M. hyopneumoniae (H1) and the other two were infected with M. hyopneumoniae (H2 and H3), as reported by the herd veterinarian and the presence of MLCL, suggestive of enzootic pneumonia. Moreover, the M. hyopneumoniae -status of the three farms was confirmed by testing bronchus-alveolar lavage fluid (BALF) and nasal turbinate (NT) samples with qPCR [ 5 , 6 ]. The free farm is a nucleus breeding herd of 650 sows, farrow-to-finish system, free of A. pleuropneumoniae and with vaccination against Influenza virus, Porcine Circovirus, Parvovirus, Leptospirosis, Erysipelas, and G. parasuis .…”

Section: Methodsmentioning

confidence: 99%

“…Gene fragments of M. hyopneumoniae (p102), M. hyorhinis (p37), and M. flocculare (fruA) from DNA samples were quantified by qPCR as described elsewhere [ 6 ], with modifications [ 5 ]. The standard curve with serial dilutions of constant ratio equal to 10 (10 7 –10 1 ) was based on a synthetic DNA (GBlock ® , IDT, USA) containing fragments of 150 base pairs (bp) ( p102 ), 101 bp ( p37 ) and 119 bp ( fruA ), used as positive control [ 5 ]. All samples were tested in duplicate, and the results were only accepted for those with a standard deviation lower than or equal to 0.5 cycle [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Although considered commensal bacteria in the respiratory tract of swine, M. hyorhinis has shown to be associated with M. hyopneumoniae infection and PRDC [ 1 , 5 ]. In addition, it is a well-known cause of polyserositis and polyarthritis in piglets, and less often is associated with pneumonia, otitis, conjunctivitis, and abortion [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it is a well-known cause of polyserositis and polyarthritis in piglets, and less often is associated with pneumonia, otitis, conjunctivitis, and abortion [ 1 ]. Recently, M. flocculare , another commensal bacterium in swine, has shown a negative correlation with the extension of macroscopic lung consolidation lesions (MLCL) when associated with M. hyopneumoniae infection [ 5 ]. Since the detection of one decreased the probability of detecting the other, it is possible that M. hyopneumoniae infection changes the environment in the respiratory tract, thereby creating unfavorable conditions for M. flocculare [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Mycoplasma hyopneumoniae natural infection on the respiratory microbiome diversity of finishing pigs

Sonálio

Almeida

Mechler-Dreibi

et al. 2022

Vet Res

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Mycoplasma (M.) hyopneumoniae interacts with the respiratory microbiota and facilitates colonization of other pathogens. The present study investigated the pulmonary and nasal microbiota of M. hyopneumoniae-infected and M. hyopneumoniae-free pigs. Sixty-six pigs from three commercial herds were selected at the end of the finishing phase: 44 originated from two M. hyopneumoniae-positive herds and 22 from a M. hyopneumoniae-negative farm. At the slaughterhouse, samples of nasal turbinate (NT) and bronchus-alveolar lavage fluid (BALF) were collected. DNA was extracted with a commercial kit and the infection status was confirmed by qPCR. All samples from the same herd were pooled, and next-generation sequencing based on the hypervariable region V3–V4 of the 16 s bacterial rDNA was performed. Data analysis included the taxonomic analysis, Alpha diversity indexes, and Principal coordinates analysis (Pcoa) using Jaccard, Bray–Curtis, Weighted Unifrac, and Unweighted Unifrac distances. All pigs from the infected herds tested PCR positive for M. hyopneumoniae, whereas all pigs from the negative farm were negative. There was a greater diversity of microorganisms in BALF when compared to NT samples in all the farms. BALF samples from infected animals showed higher abundance of M. hyopneumoniae than NT samples and a predominance of Pasteurella multocida among the main species identified, which was also abundant in the M. hyopneumoniae-free herd. PCoa diagrams indicated that for most of the samples, dissimilarity on bacterial composition was observed, regardless of infection status and sample type. Therefore, the lung microbiota was modulated by M. hyopneumoniae infection, which could play a role in the pathogenesis of M. hyopneumoniae-disease.

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Section: Discussionsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Mycoplasma hyopneumoniae natural infection on the respiratory microbiome diversity of finishing pigs

Sonálio

Almeida

Mechler-Dreibi

et al. 2022

Vet Res

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Perturbations of gut microbiome and metabolome of pigs infected with Mycoplasma hyorhinis

et al. 2023

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BackgroundMycoplasma hyorhinis is a prevalent respiratory pathogen in swine, causing significant economic loss to pig producers. There is growing evidence that respiratory pathogen infections have a large impact on intestinal microecology. To study the effect of M. hyorhinis infection on gut microbial composition and metabolome profile, pigs were infected with M. hyorhinis. Metagenomic sequencing analysis was performed of fecal samples and a liquid chromatography/tandem mass spectrometry (LC–MS/MS) analysis of gut digesta was made.ResultsPigs infected with M. hyorhinis had enriched Sutterella and Mailhella, and depleted Dechloromonas, Succinatimonas, Campylobacter, Blastocystis, Treponema, and Megasphaera. The pigs infected with M. hyorhinis also had greater abundances of bacterium_0_1xD8_71, Ruminococcus_sp__CAG_353, Firmicutes_bacterium_CAG_194, Firmicutes_bacterium_CAG_534, bacterium_1xD42_87, and lower abundances of Chlamydia_suis, Megasphaera_elsdenii, Treponema_porcinum, Bacteroides_sp__CAG_1060, Faecalibacterium_prausnitzii. Metabolomic analysis revealed that some lipids and lipid‐like molecules increased in the small intestine, whereas most lipids and lipid‐like molecule metabolites decreased in the large intestine. These altered metabolites induce changes in intestinal sphingolipid metabolism, amino acid metabolism, and thiamine metabolism.ConclusionThese findings demonstrate that infection with M. hyorhinis can alter the gut microbial composition and metabolite structure in pigs, which may further affect amino acid metabolism and lipid metabolism in the intestine. © 2023 Society of Chemical Industry.

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Pathological analysis and etiological assessment of pulmonary lesions and its association with pleurisy in slaughtered pigs

Arruda,

Malcher,

Petri

et al. 2024

Veterinary Microbiology

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Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

Cited by 8 publications

References 32 publications

Influence of Mycoplasma hyopneumoniae natural infection on the respiratory microbiome diversity of finishing pigs

Influence of Mycoplasma hyopneumoniae natural infection on the respiratory microbiome diversity of finishing pigs

Perturbations of gut microbiome and metabolome of pigs infected with Mycoplasma hyorhinis

Pathological analysis and etiological assessment of pulmonary lesions and its association with pleurisy in slaughtered pigs

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