Cytokeratin 20 Immunoreactivity in Renal Oncocytomas

Stopyra, Gary A; Warhol, Michael J.; Multhaupt, Hinke A.B.

doi:10.1177/002215540104900712

Cited by 31 publications

(20 citation statements)

References 4 publications

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“…Interestingly, a recent brief report demonstrated 80% (12/15 cases) CK 20 positivity in renal oncocytomas (18). Their results appear contradictory to our findings of 0% (0/21 cases) CK 20 positivity in renal oncocytomas.…”

Section: Discussioncontrasting

confidence: 99%

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Cytokeratins 7 and 20 Immunoreactivity in Chromophobe Renal Cell Carcinomas and Renal Oncocytomas

Kothari

Wheeler

et al. 2002

Mod Pathol

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Chromophobe renal cell carcinomas and renal oncocytomas share morphologic similarities and may present a diagnostic challenge on routine hematoxylin-eosin staining. Currently recommended additional studies of Hale's colloidal iron staining and electron microscopy are often difficult to interpret and technically challenging and may not be readily available. Previous studies have reported conflicting results with regard to the cytokeratin 7 staining pattern in chromophobe renal cell carcinomas and renal oncocytomas. Cytokeratin 20 expression in chromophobe renal cell carcinomas has not previously been studied. Formalin-fixed paraffinembedded tissue of 11 chromophobe renal cell carcinomas and 21 renal oncocytomas were retrieved from the archived files (1984 -2000) of four teaching hospitals. Of the 11 chromophobe renal cell carcinomas, eight stained positive (73%) for cytokeratin 7, one stained focally positive (9%), and two cases (18%) were completely negative. Cytokeratin 7 staining of the 21 oncocytomas revealed 4 positive (19%), 7 focally positive (33%), and 10 negative cases (48%). Cytokeratin 20 was uniformly negative on all 11 cases of chromophobe renal cell carcinomas and all 21 cases of oncocytomas. Cytokeratin 7 does not appear to show the consistent immunoreactivity in chromophobe renal cell carcinomas and renal oncocytomas, as has been previously suggested. Cytokeratin 20 immunostaining in chromophobe renal cell carcinomas and renal oncocytomas is uniformly negative. Despite the technical and interpretive challenges of Hale's colloidal iron, it is still the most useful stain in differentiating chromophobe renal cell carcinomas from renal oncocytomas.

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Section: Discussioncontrasting

confidence: 99%

“…The CK 20 profile for chromophobe renal cell carcinomas has not previously been studied. A recent brief report on CK 20 staining in oncocytomas demonstrated 80% positivity with variable patterns and distributions (18). Consecutive paraffin sections were cut at 4 m and placed on poly-L-lysine slides.…”

mentioning

confidence: 99%

Cytokeratins 7 and 20 Immunoreactivity in Chromophobe Renal Cell Carcinomas and Renal Oncocytomas

Kothari

Wheeler

et al. 2002

Mod Pathol

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“…A similar perinuclear staining pattern was also described earlier in oncocytomas positive for cytokeratin 20 staining, 36 and colloidal iron staining (as mentioned above). 27 Stopyra et al 40 hypothesized that such peculiar staining pattern corresponds to large cytoplasmic condensations of intermediate filaments or globular filamentous bodies, described so far only in pathological processes.…”

Section: Discussionmentioning

confidence: 99%

The diagnostic and prognostic utility of claudin expression in renal cell neoplasms

et al. 2008

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This study evaluated the expression patterns of claudins 1, 3, 4, 7, and 8 in human renal cell carcinomas and oncocytomas and correlated expression with patient prognosis. Tissue microarrays were created from paraffinembedded tissue samples from 141 patients with renal cell carcinomas or oncocytoma (90 clear cell, 22 papillary, 17 chromophobe renal cell carcinomas, and 12 oncocytomas). The staining pattern for claudins 3, 4, 7, and 8 was membranous and/or cytoplasmic, whereas claudin 1 was predominantly membranous in both nonneoplastic renal tissue and tumors. Negative to weak claudin 3 staining was predominantly detected in Fuhrman's grade 1 and 2 clear cell renal cell carcinomas (78%; P ¼ 0.016), suggesting that upregulation of claudin 3 potentially occurs concomitantly with increasing grade of clear cell renal cell carcinomas. In addition, Kaplan-Meier univariate analysis showed a significant inverse correlation between moderate to strong claudin 3 and 4 expression with overall survival in clear cell renal cell carcinomas (P ¼ 0.038 and P ¼ 0.031). Moderate to strong claudin 7 expression was significantly more common in chromophobe renal cell carcinomas (94%) than in oncocytomas (55%; P ¼ 0.041). Claudin 8 staining was moderate to strong in 92% of oncocytomas, which differentiated them from papillary and clear cell renal cell carcinomas (14 and 12%; both Po0.0001). Only negative to weak claudin 8 staining was detected in all chromophobe renal cell carcinomas, whereas there were no claudin 8 negative oncocytomas and 8% exhibited a weak staining pattern (Po0.0001). Due to their distinctive expression patterns, claudins 7 and 8 can be used as useful immunohistochemical markers for the separation of chromophobe renal cell carcinomas from oncocytomas, whereas claudins 3 and 4 may serve as indicators of prognosis in clear cell renal cell carcinomas.

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“…However, the results reported are, at least as far as the keratin expression profile of ROs is concerned, still limited and partly contradictory. For example, keratin 7 immunoreactivity has been detected in 8-100% [12,17,19,33,38] and keratin 20 in 0-80% of ROs [12,33,38]. Hence, we decided to apply a high throughput tissue microarray technique using 22 different mouse monoclonal antibodies to evaluate the keratin expression profiles…”

Section: Introductionmentioning

confidence: 99%

Keratin immunohistochemistry in renal cell carcinoma subtypes and renal oncocytomas: a systematic analysis of 233 tumors

et al. 2004

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Keratin immunohistochemistry represents a widely applied differential diagnostic tool in surgical pathology. To investigate the value of keratin subtyping for the diagnosis among histological subtypes of renal cell carcinoma and oncocytomas, we performed a detailed immunohistochemical study, applying 22 different monoclonal keratin antibodies on a large series of 233 renal tumors [125 conventional, 22 chromophobe, and 20 papillary (12 type-1, 8 type-2 tumors) cancers and 66 oncocytomas] using a tissue microarray technique. Immunoreactivity for keratin 7, 8, 18, and 19 was present in all tumor entities, albeit in varying quantities. With antibodies directed against keratins 8 and 18, oncocytomas showed a distinct perinuclear and punctate dot-like pattern, which was not observed in renal cancer specimens. The only tumors showing immunoreactivity for keratin 20 were two type-2 papillary cancers. All other monospecific keratin antibodies yielded consistently negative results. Overall, in contrast to some recent publications, keratin subtyping generally appeared to be of additional value only for the differentiation of renal epithelial tumors. Hence, with respect to differential diagnostic value, Hale's colloidal iron stain and vimentin immunostaining are still the most useful tools in renal tumor pathology.

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Cytokeratin 20 Immunoreactivity in Renal Oncocytomas

Cited by 31 publications

References 4 publications

Cytokeratins 7 and 20 Immunoreactivity in Chromophobe Renal Cell Carcinomas and Renal Oncocytomas

Cytokeratins 7 and 20 Immunoreactivity in Chromophobe Renal Cell Carcinomas and Renal Oncocytomas

The diagnostic and prognostic utility of claudin expression in renal cell neoplasms

Keratin immunohistochemistry in renal cell carcinoma subtypes and renal oncocytomas: a systematic analysis of 233 tumors

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