Summary Karyomorphological studies were carried out in seven accessions of Chlorophytum borivilianum collected from the states of Rajasthan and Madhya Pradesh. All the accessions were found to be tetraploid with 2nϭ28 chromosomes which were resolved into 7 groups, each comprising 7 homologous chromosomes. All the accessions had submetacentric chromosomes in high proportion while metacentrics, telocentrics and subtelocentrics recorded were very few in number. Nucleolar chromosomes were recorded in 5 (PBL-1, PBL-2, PBL-4, PBL-5 and PBL-7) out of the 7 accessions studied. The karyotypes of all the accessions were more or less symmetrical. The role of karyotypic variation in speciation and evolution of the genus Chlorophytum has been discussed in detail.