Cytogenotoxicity of rice crop water after application of the tricyclazole fungicide

Wandscheer, Alana Cristina Dorneles; Marchesan, Enio; Tedesco, Solange Bósio; Frescura, Viviane Dal-Souto; Soares, Camille Flores; Londero, Guilherme Pilar; Teló, Gustavo Mack; Hansel, Dâmaris Sulzbach Santos

doi:10.1590/0001-3765201720150536

Cited by 7 publications

(10 citation statements)

References 18 publications

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“…The studied pesticides had practically no effect on the MI of the root apex meristem of 2-day-old wheat seedlings. A similar lack of cytotoxic effect of the tricyclazole fungicide tricyclazole used in rice cultivation is described by Wandscheer et al [23]. Using the Allium cepa test, the authors showed, that tricyclazole causes chromosomal abnormalities, but does not signi cantly affect the MI.…”

Section: Discussionsupporting

confidence: 65%

“…For example, the systemic fungicide Tricyclazole, used on irrigated rice crops, has genotoxic activity: increases the frequency of chromosomal abnormalities [23]. The systemic herbicide Roundup, used to control weeds in cereal crops, increases the number of metaphase disorders in barley, which is due to the negative effect on the mitotic spindle formation [24].…”

Section: Introductionmentioning

confidence: 99%

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Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

Menzyanova

Shishatskaya

Pyatina

et al. 2020

Preprint

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Using the example of 2-day-old Triticum aestivum seedlings, grown in hydroponic culture, the cytological effects of pesticides with various mechanisms of the damaging were studied: the herbicides metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl and the fungicides tebuconazole, epoxiconazole, and azoxystrobin. All pesticides showed a dose-dependent inhibitory effect on the development of the roots of Triticum aestivum, but did not affect the mitotic activity and did not reduce the mitotic index of root apex meristem cells. Herbicides and fungicides affected the duration of metaphase, anophase, prophase, and cytokinesis activity in the root apex meristem of the wheat, and these changes were specific for the six studied preparations. Under the influence of all pesticides, the number of abnormal cells increased significantly, to the greatest extent (10–12 times compared with control - distilled water) under the influence of herbicides tribenuron-methyl and phenoxaprop-ethyl and fungicides azoxystrobin and epoxiconazole; to a lesser extent - under the influence of metribuzin and tebuconazole (6–7 times). For all studied pesticides the manifestation of cyto- and genotoxicity, including both clastogenic and aneugenic effects, was revealed in relation to the non-target plant Triticum aestivum. Under the influence of pesticides, structural anomalies, caused by chromosome breakdown (bridges, fragments, micronuclei) and spindle damage (lagging chromosomes and their fragments, uneven chromosome separation) were detected in the cells of the Triticum aestivum root apex meristem.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

Menzyanova

Shishatskaya

Pyatina

et al. 2020

Preprint

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“…The Allium cepa roots were placed in contact with rice crop water to check for possible chromosomal MI abnormalities by Wandscheer et al (2017). The data obtained indicates that the application of the tricyclazole fungicide leads to an increase in the genotoxic activity in the rice crop water, through the appearance of chromosomal abnormalities, without, however, causing significant effects on the MI.…”

Section: Cyto-genotoxicity Of Fertilizers Monitored By Allium Root Timentioning

confidence: 99%

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Firbas²,

et al. 2018

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A general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student's t, and the nonparametric tests Kruskal-Wallis and Mann-Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug.

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“…One of the possibilities for studying the genotoxic effect of pesticides is using plants as test objects. Plants are recognized as excellent genetic models in detecting mutagenic environmental agents and are often used for biomonitoring studies [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Effects of the Neonicotinoid Insecticides Nuprid 200 SL and Calypso 480 SC on Plant Model System

Stoyanov,

Vasileva,

Staykova

et al. 2023

OBM Genet

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Data from the present study indicate that the neonicotinoid insecticides Nuprid 200 SL (with the active substance imidacloprid) and Calypso 480 SC (with the active substance thiacloprid) reduce the mitotic activity by inhibiting cell division and increasing chromosomal aberrations in the cells of the root apical meristem of Allium cepa L. The tested solutions of pesticides cause a wide range of anomalies associated with disorders in forming the cell's division apparatus and the integrity of chromosomes. The comparative analysis of their mutagenic action shows a higher genotoxic potential of Calypso 480 SC. The established indices of chromosomal abnormalities for Calypso 480 SC and Nuprid 200 SL are 1.07 ± 0.38% and 0.70 ± 0.41% respectively. The mitosodepressant effect and a large number of chromosomal aberrations and mitotic abnormalities in Allium cepa cells when treated with Nuprid 200 SL and Calypso 480 SC are evidence of their high toxic potential and the significant risk of environmental pollution by their use in agriculture. Parts of this work was published in abstract form and presented as an oral presentation at the IV International Agricultural, Biological & Life Science conference, Edirne, Turkey, 2022 August 29-31, [1] and as a poster presentation at the 5th Balkan Scientific Conference on Biology, Plovdiv, Bulgaria, 2021 April 15-16 [2].

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Cytogenotoxicity of rice crop water after application of the tricyclazole fungicide

Cited by 7 publications

References 18 publications

Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Cytogenetic Effects of the Neonicotinoid Insecticides Nuprid 200 SL and Calypso 480 SC on Plant Model System

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