Cytogenetics of tissue culture regenerated hybrids of Triticum tauschii × Secale cereale

Abstract

togotlrtie.s Soc.tiotl, Otto~t'o Ro.srtrrc.11 Srtrriotl, Rc.sc~ert1~11 Bt.trt1c.h A,qt-ic.~rlrlrt.c~ C'ot~otlo. Otro~t.tr, O t~r . . ccrtloclo KIA OCh Received Scptcmbc~. 6. I983 Rccised manuscript rcccivcd February 2.1 . I984 FE[)AK. G . 1984. Cytogcnctics of tissue culturc regenerated hybrids of 7'r-iric~lrtt1 rtr/r.\c~l~ii i i Scc.olo c,crc)olr. Can. J . Genet. Cytol. 26: 387-386. Immature inflorcsccnces of Tt-iric.1rtt1 rtrlr.\c.l~ii (Coss. ) Schmal. Scc~c/lc) c~c~t-c~c~lc L. cv. 'Puma' hybrids Mcrc induced to callus on Kao's medium supplcmcntcd with 5 mg/L 2-4-diclllorophc~~oxyacctic acid (2.1-D). Plantlets with diploid and tctraploid C~T O I I I O S O I I I~ number\ ucrc \ubscqucntly regencrateci from the callus. Chromo\omc pairing in the diploid rcgcncrants and tetraploid a~llphiploids W;IS incomplctc and the plants ucrc sterile. KO\ \tor-d.\. I'rlrlc ~r t t i . Scc olo. chrorno\omc palnng. callu\. t~\ \ u c culturc FEI),IK. G . 1981. C!.togcnctics of tissuc culture regenerated hybrid5 01' 7't-iric~lrt?1 rolr.\c~liii x Scc~olo c~ot.ct/lc. Can. J . Genet. Cytol. 26: 382-3236. On a induit dcs i n t l o~. c~c c n c c~ imrnaturcs. hybridcs dc 7't.iric~lrt~i rtl~r.\c~l~/i tCo\\. ) Schmal ct du cultivar 'Puma' dc Sc)c~olc c.c~t.c~olo L . , i prociuirc clc5 cal\ dans Ic nlilicu cle Kao \uppl6menti. avcc I'acidc 2-1-dichloropl~CnoxyacCtiquc t 2.1-1)) i la concentration dc 5 mgIL. On 3 ensuite rCpCn6rC dch planti~les diploides ct td~.aplo'~dcs ii partir dc ecs caI\. L'appariemcnt chromoso~niquc chcr le5 plantulcs diplo'idcs ct c h c~ Ic\ amphiplo~de\ tGtraploldc\ Ctait incomplct ct Ics plantes Ctaicnt st6riles. i2lor.c (.Ic;.\: Tt.iric./rtn. Soc~olo. apparicmcnt chromo~omiquc. ca1. cu1ti11.c dc t i5su.

A trigeneric hybrid between Hordeum, Aegilops, and Secale

“…This is in agreement with the general plant morphology of trigeneric hybrids obtained between Hordeum, Triticum, and Secale, as well as with those obtained between Hordeum-Triticum and Triticum-Secale hybrids or amphiploids (Fedak and Armstrong 1980;Martin and Sanchez-Monge Laguna 1982;Fedak 1984).…”

“…This result is in agreement with those obtained in the hybrids between H . chilense and A. squarrosa (Martin 1983), T. tauschii and S. cereale (Fedak 1984), and H. chilense and S. cereale (Finch and Bennett 1980;Pohler and Schrader 1986). However, this result is in contrast with the findings of Thomas and Pickering (1985) of homoeologous pairing in the H .…”

The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L. em. thell) callus

“…The interaction of cytoplasmic and nuclear genes with growth regulators in the culture medium was discussed by Mathias et al [9,10]. Coconut milk has frequently been used to improve the responses of cereal tissues in culture [4,8,12]. Addition of CM to media provides a complex of undefined 'growth factors' which certainly include vitamins, amino acids and cytokinins.…”

“…Callus cultures have been initiated from various explants of wheat [ 1,2,3,4,12,16,17] but most reports of regeneration in culture have been from caUi initiated from immature embryos [5,6,7,8,13,15]. Several authors [7,8,13,15] have recognised that among wheat genotypes there is significant variation in the culture behaviour of embryo derived calli.…”

Plant regeneration and chromosome instability in tissue culture of Elymus canadensis × E. trachycaulus F1 hybrid

“…Chromosome doubling of plants derived from tissue culture has been reported in E. canadensis (Park and Walton 1989), Echinoclova orizicola (Takahashi et al 1984), Pennisetum americanum (Swedlund and Vasil 1985), and hybrids of Triticum tauschii x Secale cereale (Fedak 1984) and Triticum crassum x Hordeum vulgare (Nakamura et al 1981). I n E. canadensis, E. orizicola, and P. americanum the doubled plants were fully or partially albino.…”