The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L. em. thell) callus

Mathias, R. J.; Simpson, Eric S.

doi:10.1007/bf00043918

Cited by 86 publications

(39 citation statements)

References 15 publications

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“…Besides, there are several other reports concerning the promotive effects of cytokinin on organogenesis from wheat cell cultures.2,10,23-25) Maddock et al11) have observed that the addition of 10% coconut milk (CM) to the callus induction medium significantly promoted subsequent shoot regeneration, and the same effect of CM has been identified in another study.3) It is supposed that the promotive effects of additives such as CM on the regenerative potential of callus cultures may be largely due to unidentified cytokinin activity. 26) In the present study, many embryoid-like structures were observed during the early period of callus induction, especially in the presence of kinetin. From the above results, it is considered that exogenous cytokinin in callus induction medium plays an essential role in embryoid formation from cultures, because this is consistent with a possible requirement in early zygotic embryo initiation and development.22) Thus, it is possible to say that the effect of kinetin indicated in this study originated in induction of embryoid formation in the early period of callus formation; however, these de novo-formed immature embryoids may not develop into mature ones and precociously regenerated into vigorous shoots.…”

Section: Discussionsupporting

confidence: 51%

Effects of plant growth regulators on the response in immature wheat embryo culture.

Liu

Misoo

Kamijima

et al. 1990

Plant tissue culture letters

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The purpose of this study was to investigate the effects of 2,4-D, 6-furfurylamino purine (kinetin), and abscisic acid (ABA) on the response of cultured immature wheat (Triticum aestivum L.) embryos, by evaluating direct germination, callus growth, shoot regeneration and its development.The reduction of 2, 4-D concentration or the addition of kinetin to callus induction media promoted direct germination of cultured embryos, whereas the addition of ABA suppressed it extremely. Callus growth was promoted by reducing 2, 4-D concentration or by adding ABA at lower concentrations, though these alterations did not affect the frequency of callus formation.Concentrations of 2, 4-D in callus induction media did not affect the frequency of subsequent shoot regeneration from calli; however, the regenerated shoots developed rapidly when calli were induced at lower 2, 4-D. The addition of kinetin or ABA significantly improved the shoot-forming ability of callus cultures, especially in less responsive cultivars.Regenerative potential of callus cultures varies significantly depending on the donor cultivars or organs. Regeneration in wheat has been demonstrated for numerous cultivars by culturing immature embryos.1-8) However, callus cultures of some cultivars produce few shoots,4, 5, 9-13) and even attempts to induce callus from several other cultivars have failed.4) Consistent shoot formation occurs for most cultivars when 2, 4-D is used as the auxin source.3-5,11) However, no callus formation was observed when IAA or naphthalene acetic acid (NAA) was used as the only auxin source.3) Also, it has been known that the optimum concentration of 2, 4-D for callus formation differs among cultivars, and regenerative potential varies among calli induced at different 2, 4-D concentrations.14) Recently, with increased understanding of hormonal and nutritional requirement of various morphogenetic processes, further improvement has been achieved in wheat tissue culture. The frequency of embryogenic callus formation from immature wheat embryos increased by using the combination of 2, 4-D, NAA, and 6-benzyladenine (6-BA) rather than 2, 4-D only,15) and pollen embryoid formation was promoted by ABA in wheat anther culture.16,17) In the present study, we report that efficient shoot formation could be realized especially in less responsive cultivars by applying different hormonal combinations in callus induction medium. Materials and MethodsFour cultivars, Chinese Spring (C.S.), Aobakomugi, Normn No. 61, and Nongda 146 were chosen for their response to tissue culture in the previous study.18) Immature caryopses were taken from main spikes at 10 to 14 days postanthesis and were surface-sterilized first in 70% ethanol for 10 sec, then in 1.5% sodium hypochlorite solution with a drop of Tween 20 for 10 min, followed by several rinses with sterile distilled water. Ten embryos, which were selected for uniformity of about 1 mm in diam-

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Section: Discussionsupporting

confidence: 51%

Effects of plant growth regulators on the response in immature wheat embryo culture.

Liu

Misoo

Kamijima

et al. 1990

Plant tissue culture letters

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“…For induction response, results in the literature range from 92-100% (Ozgen et al 1996), 20-100% (Machii et al 1998) and for regeneration capacity, from 29 -100% (Maddock et al 1983); 0-60% (Mathias and Simpson 1986); 0-90% (Machii et al 1998); and 28-60% (Li et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

Vendruscolo¹,

Schuster²,

Negra³

et al. 2008

CBAB

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-The distinction of genotypes responsive to tissue culture and the development of an efficient regeneration system are the first steps towards transgenic plant production. Nine Brazilian wheat (Triticum aestivum L.) genotypes were cultivated in vitro to evaluate the embryogenetic capacity. The explants (immature zygotic embryos) were tested in two different culture media, MS (Murashige and Skoog 1962) and modified MS -MMS (Zhou et al. 1995)

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“…There are some reports on growth and morphogenesis is remarkably affected by supplementing tissue culture medium with plant growth regulators including auxins and cytokinins. Various studies have investigated the factors which affect the plant regeneration in tissue culture including composition of culture medium, genotype and environmental factors (Fennel et al, 1996;Uppal et al, 1996;Mathias and Simpson, 1986). In this study, calli were used that derived from immature and matured seeds of four wheat varieties and also evaluated various culture conditions for obtaining high frequencies of somatic embryogenesis and plant regeneration in in vitro systems.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Somatic Embryogenesis by Mature and Immature Seeds in Wheat (Triticum aestivum L.)

Saha¹,

Islam²,

Islam³

et al. 2017

JBLS

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A suitable plant regeneration system has been established using 3-4 weeks old calli derived from immature and mature seeds of four wheat varieties viz. Pavon 76, Akbar, Barkat, and Kanchan. As plant growth regulators, various auxins (2,4-D, BAP and IAA) either single or in combination were used in MS medium. The variety Pavon 76 showed maximum (72.25%) callus induction and Akbar exhibited the lowest (37.78%) from calli derived from immature seeds. Hormonal effects on callus induction were evaluated and significant results were found in case of genotypes at P <0.01. Out of four genotypes, the highest frequency of plant regeneration was recorded in Pavon 76 (67.00%) and lowest in Kanchan (43.10%) when 1.5 mg/l BAP and 0.5 mg/l IAA was added in the medium. It was observed that Pavon 76 produced highest number of green plants than others. For mature seeds all of the mentioned genotypes showed significant difference with maximum frequency of callusing in Pavon 76 (69.57%) in MS + 2.5 mg/l 2,4-D followed by Kanchan (60.84%), Barkat (52.73%), and Akbar (47.19%). For plant regeneration, Pavon 76 also showed best performance (64.36%) in MS + 2.0 BAP + 1.0 mg/l IAA, using calli derived from mature seeds. The other genotypes Barkat, Kanchan and Akbar exhibited 59.44, 52.71 and 52.32% regeneration in the same medium respectively. Here, the lowest regeneration (40.63%) was found in Akbar. In this case, it was aimed to establish a suitable protocol for in vitro callus induction and regeneration for advance biotechnological research on wheat in Bangladesh.

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The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L. em. thell) callus

Cited by 86 publications

References 15 publications

Effects of plant growth regulators on the response in immature wheat embryo culture.

Effects of plant growth regulators on the response in immature wheat embryo culture.

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

Enhancement of Somatic Embryogenesis by Mature and Immature Seeds in Wheat (Triticum aestivum L.)

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