Cytogenetics of four Omophoita species (Coleoptera, Chrysomelidae, Alticinae): A comparative analysis using mitotic and meiotic cells submitted to the standard staining and C-banding technique

Almeida, Mara Cristina de; Campaner, Carlos; Cella, Doralice Maria

doi:10.1016/j.micron.2009.02.011

Cited by 13 publications

(17 citation statements)

References 39 publications

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“…In the Chrysomelidae, the constitutive heterochromatin occurs predominantly in the pericentromeric region (Almeida et al, , 2009Mello et al, 2014). This same pattern was also observed in the autosomal chromosomes of Coleoptera (Virkki, 1983, Schneider et al, 2006Mello et al, 2014).…”

Section: Introductionsupporting

confidence: 59%

“…The chromosomes of Chrysomelidae also exhibited this pattern (Virkki, 1983;Almeida et al, 2006Almeida et al, , 2009Mello et al, 2014); however, they show a lower amount of heterochromatin compared with other groups, such as Elateridae and Tenebrionidae (Juan & Petitpierre, 1989;Rozek et al, 2004;Schneider et al, 2006).…”

Section: Discussionmentioning

confidence: 92%

“…The distribution of constitutive heterochromatin has been described for different families and subfamilies of Coleoptera, in which the C-bands are generally located in the pericentromeric region of the autosomal chromosomes (Vidal et al, 1977;Virkki, 1983;Juan & Petitpierre, 1989;Rozek & Lachowska, 2001;Zacaro et al, 2004;Bione et al, 2005;Almeida et al, 2009). The chromosomes of Chrysomelidae also exhibited this pattern (Virkki, 1983;Almeida et al, 2006Almeida et al, , 2009Mello et al, 2014); however, they show a lower amount of heterochromatin compared with other groups, such as Elateridae and Tenebrionidae (Juan & Petitpierre, 1989;Rozek et al, 2004;Schneider et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Comparative cytogenetic analysis in 13 tortoise beetles (Coleoptera: Chrysomelidae: Cassidinae) from Brazil

Lopes¹,

Fernandes²,

Schneider³

2016

Eur. J. Entomol.

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ISSN (online): 1802-8829http://www.eje.cz fi nding was corroborated by a phylogenetic study using molecular data (Gómez-Zurita et al., 2008).Cytogenetically, only 117 Cassidinae species belonging to 13 tribes have been investigated to date (for revision see De Julio et al., 2010). The tortoise beetles are considered as a group with conserved karyotype, in which approximately 52% of the analyzed species showed 2n = 16 + Xy p , with metacentric and/or submetacentric chromosomes (De Julio et al., 2010). However, cytogenetic records also demonstrated that the diploid numbers can range from 2n = 16 to 2n = 51, as well the sex chromosome systems, which can be either simple -Xy p , X0, Xy, Xy c , Xy r , neoXY, or multiple -Xyy p , X p neoXneoY p , X p neoXneoy p , neoX p neoyy p , neoX p1 neoX p2 neoXneoy p , neoX p1 neoX p2 neoXneoy, X p1 X p2 -neoXneoy (for review see De Julio et al., 2010). These variations in the diploid number and sex chromosome systems are more or less frequent according to the tribe considered. In Cassidini and Mesomphaliini, 44% and 100% Abstract. In the present work, we have characterized the chromosomes of 13 Cassidinae beetles, belonging to four tribes, the broad aim being to increase the cytogenetic data and establish the mechanisms involved in chromosome evolution of this subfamily, which appear to be conserved karyotypically, i.e. 2n = 16 + Xy p . The analysis of mitotic and meiotic cells revealed a high diversity of diploid numbers (2n = 18, 2n = 22, 2n = 26, 2n = 32, 2n = 36, 2n = 40, 2n = 42), and the presence of sex chromosome system of the Xy p type in most species, with the exception of two representatives that exhibited Xy r and XY systems. C-banding showed constitutive heterochromatin predominantly localized in the pericentromeric region of the chromosomes, but differences regarding the number of chromosomes with positive C-bands, intensity of the blocks, and presence of additional bands in autosomes and/or sex chromosomes were observed among the species investigated. Our data revealed that the karyotype 2n = 16 + Xy p does not occur in all 13 tribes of the Cassidinae characterized cytogenetically, seeming to be only a shared feature among the species of the Cassidini. Variations in the C-band pattern, mainly in closely related species, suggest that the interspecifi c karyotype diversifi cation occurred as a result of changes in the quantity and distribution of constitutive heterochromatin. The occurrence of the Xy p sex chromosome system in the tribe Mesomphaliini, which showed the highest diversity of simple and multiple systems among the coleopteran as a whole, reinforces the view that derived systems originated by chromosome rearrangements involving the Xy p ancestral system.

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Section: Introductionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Comparative cytogenetic analysis in 13 tortoise beetles (Coleoptera: Chrysomelidae: Cassidinae) from Brazil

Lopes¹,

Fernandes²,

Schneider³

2016

Eur. J. Entomol.

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“…In Oedionychina, species of the Omophoita possess 2n = 22 chromosomes and the meiotic formula 10II+X+Y. The occurrence of asynaptic giant sex chromosomes during meiosis is a notable karyotypic feature in this group [Smith and Virkki, 1978;Almeida et al, 2009Almeida et al, , 2010Mello et al, 2014]. Sex chromosome differentiation is independent among animal groups, and, in insects, the innumerous systems differ in type and morphology of chromosomes within each evolutionary lineage [Kaiser and Bachtrog, 2010].…”

mentioning

confidence: 99%

Accumulation of Transposable Elements in Autosomes and Giant Sex Chromosomes of Omophoita (Chrysomelidae: Alticinae)

Rosolen

Vicari

Almeida

2018

Cytogenet Genome Res

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Coleoptera is the most diverse order among insects, and comparative molecular cytogenetic studies in this group are lacking. The species of Omophoita (Oedionychina) possess a karyotype of 2n = 22 = 10II+X+Y. They are interesting models for evolutionary cytogenetic studies due to giant sex chromosomes which are asynaptic during meiosis. Transposable elements (TEs) confer plasticity and mobility to genomes and are considered hotspots for DNA double-strand breaks and chromosomal rearrangements. The objective of the present study was to verify the role of TEs in the karyotype and in the size expansion of the giant sex chromosomes in Omophoita. Thus, different TEs were characterized in the Omophoita genome and localized in the chromosomes by fluorescence in situ hybridization (FISH). The DNA sequencing data revealed identity with TE families Tc1/Mariner and RTE/L1-56_XT. FISH showed signals of all TEs in the karyotypes and a high accumulation in the sex chromosomes of the 3 Omophoita species analyzed. These data suggest that the genome size expansion and the origin of the giant sex chromosomes of Omophoita are due to an intensive genomic invasion of TEs, as those characterized here as Tc1/Mariner-Ooc and RTE-Ooc. Differences in the chromosomal location of the TEs among the 3 species indicate that they have participated in the karyotype differentiation in Omophoita.

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“…Given that Coleoptera is of great ecological significance coleopters are widely used in several studies, including those that evaluate the ecology, morphophysiology, genetic structure, and phylogeny of the order (Martínez-Navarro et al 2005, Short 2010, Soudi and Moharramipour 2012, Almeida et al 2009). Although several molecular and cytogenetic studies have been conducted, they are still scarce compared to the great diversity of the order; only 1% of the representatives have been analyzed.…”

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confidence: 99%

Evolutionary Dynamics of Tropisternus Karyotype (Coleoptera: Hydrophilidae: Hydrophilini) from Southern Brazil

et al. 2013

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SummaryThe family Hydrophilidae is the largest group of the superfamily Hydrophiloidea to which the largest aquatic Polyphaga are allocated. Phylogenetic studies based on morphological characters suggest that in Hydrophilidae, specifically in Hydrophilini, the body size is related to the group evolution. Despite these morphological analyses, cytogenetic studies of this family are scarce, and there is not a correlation of morphologic and cytogenetic data outlined for this group. In this paper, we analyzed seventeen specimens of Tropisternus mutatus sapucay Fernández & Bachman from rivers from two localities in Southern Brazil. Chromosome analyses showed 2n=18 with a sex chromosome system of the Xy p type (2n=18=16+Xy p ). All the autosomes, including the X p chromosome, were metacentric. The basal number and the possible divergence among the different Hydrophilidae species analyzed to date, as well as the phylogeny proposed for Hydrophilini, are discussed.

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Cytogenetics of four Omophoita species (Coleoptera, Chrysomelidae, Alticinae): A comparative analysis using mitotic and meiotic cells submitted to the standard staining and C-banding technique

Cited by 13 publications

References 39 publications

Comparative cytogenetic analysis in 13 tortoise beetles (Coleoptera: Chrysomelidae: Cassidinae) from Brazil

Comparative cytogenetic analysis in 13 tortoise beetles (Coleoptera: Chrysomelidae: Cassidinae) from Brazil

Accumulation of Transposable Elements in Autosomes and Giant Sex Chromosomes of <b><i>Omophoita</i></b> (Chrysomelidae: Alticinae)

Evolutionary Dynamics of <i>Tropisternus</i> Karyotype (Coleoptera: Hydrophilidae: Hydrophilini) from Southern Brazil

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