Cytogenetic Studies on Metaphase Chromosomes of Six Bivalve Species of Families Mytilidae and Veneridae (Nucinelloidea, Mollusca)

Ebied, Abdel-Basset M.; Aly, Fayza M.

doi:10.1508/cytologia.69.261

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…To date, karyological data are available for only a few species of the family Veneridae [ 10 , 11 , 24 ]. Both the chromosome numbers and the karyotypes determined in this work for Ruditapes philippinarum , R. decussatus , Venerupis corrugata , Venus verrucosa, and Dosinia exoleta are in agreement with previous results [ 12 , 13 , 25 , 26 ] and further confirm that, unlike other families within the order Veneroida in which interspecific differences in chromosome numbers have been detected, all Veneridae species have the same chromosome number, 2 n = 38. Nevertheless, the high variation in karyotype composition detected in these species indicates that speciation in Veneridae was accompanied by some chromosome rearrangements.…”

Section: Discussionsupporting

confidence: 91%

Evolutionary Dynamics of rDNA Clusters in Chromosomes of Five Clam Species Belonging to the Family Veneridae (Mollusca, Bivalvia)

Pérez-García

Hurtado

Morán

et al. 2014

BioMed Research International

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The chromosomal changes accompanying bivalve evolution are an area about which few reports have been published. To improve our understanding on chromosome evolution in Veneridae, ribosomal RNA gene clusters were mapped by fluorescent in situ hybridization (FISH) to chromosomes of five species of venerid clams (Venerupis corrugata, Ruditapes philippinarum, Ruditapes decussatus, Dosinia exoleta, and Venus verrucosa). The results were anchored to the most comprehensive molecular phylogenetic tree currently available for Veneridae. While a single major rDNA cluster was found in each of the five species, the number of 5S rDNA clusters showed high interspecies variation. Major rDNA was either subterminal to the short arms or intercalary to the long arms of metacentric or submetacentric chromosomes, whereas minor rDNA signals showed higher variability. Major and minor rDNAs map to different chromosome pairs in all species, but in R. decussatus one of the minor rDNA gene clusters and the major rDNA cluster were located in the same position on a single chromosome pair. This interspersion of both sequences was confirmed by fiber FISH. Telomeric signals appeared at both ends of every chromosome in all species. FISH mapping data are discussed in relation to the molecular phylogenetic trees currently available for Veneridae.

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Section: Discussionsupporting

confidence: 91%

Evolutionary Dynamics of rDNA Clusters in Chromosomes of Five Clam Species Belonging to the Family Veneridae (Mollusca, Bivalvia)

Pérez-García

Hurtado

Morán

et al. 2014

BioMed Research International

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“…Teredo utriculus (2n = 38) 14 Vitturi et al (1983) Family VENERIDAE Chamelea gallina (2n = 38) 4 Corni and Trentini (1986) Circe scripta (2n = 38) 3 Ebied and Aly (2004) Cyclina sinensis (2n = 36) b 11 Meretrix meretrix (2n = 38) b 3 Wu et al 2002Ruditapes aureus (V. aurea) (2n = 38) 1 Borsa and Thiriot-Quievreux (1990), Carrilho et al (2011) 1…”

Section: Discussionmentioning

confidence: 99%

“…Ruditapes decussatus (2n = 38) 5 Ebied and Aly (2004) Tapes rhomboides (2n = 38) 4 Carrilho et al (2011) 1…”

Section: Discussionmentioning

confidence: 99%

“…Venerupis rhomboides (2n = 38) 3 Insua and Thiriot-Quievreux (1992) Venus verrucosa (2n = 38) 4 Ebied and Aly (2004) a Different levels of polyploidy b In Chinese; only abstract in English T. rhomboides and V. aurea (Carrilho et al 2011) with one signal. In this present work, we reported the occurrence of at least one 5S rDNA array containing a sufficient number of repeats to yield a (weak) fluorescent signal in E. minor.…”

Section: Discussionmentioning

confidence: 99%

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Cytogenetic characterisation of the razor shells Ensis directus (Conrad, 1843) and E. minor (Chenu, 1843) (Mollusca: Bivalvia)

et al. 2012

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The European razor shell Ensis minor (Chenu 1843) and the American E. directus (Conrad 1843) have a diploid chromosome number of 38 and remarkable differences in their karyotypes: E. minor has four metacentric, one metacentric-submetacentric, five submetacentric, one subtelocentric and eight telocentric chromosome pairs, whereas E. directus has three metacentric, two metacentric-submetacentric, six submetacentric, six subtelocentric and two telocentric pairs. Fluorescent in situ hybridisation (FISH) using a major ribosomal DNA probe located the major ribosomal genes on one submetacentric chromosome pair in both species; FISH with a 5S ribosomal DNA (5S rDNA) probe rendered one chromosomal (weak) signal for E. minor and no signal for E. directus, supporting a more dispersed organisation of 5S rDNA compared to the major ribosomal genes. The vertebrate telomeric sequence (TTAGGG) n was located on both ends of each chromosome, and no interstitial signals were detected. In this work, a comparative karyological analysis was also performed between the four Ensis species analysed revealing that the three European species studied so far, namely E. minor, E. siliqua (Linné 1758) and E. magnus Schumacher 1817 show more similarities among them than compared to the American species E. directus. In addition, clear karyotype differences were found between the morphologically similar species E. minor and E. siliqua. Keywords Razor shells Á Karyotype Á FISH Á 18S-5.8S-28S rDNA Á 5S rDNA Á Telomeric sequence ''E. minor was frequently treated under the name E. siliqua or as a subspecies of that taxon; however, along the Atlantic coast the two species occur sympatrically with Communicated by Heinz-Dieter Franke.

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“…In contrast, the chromosomal characterization of the venerid clams lags far beyond the knowledge achieved for other bivalve families. Classical venerid cytogenetics was limited to determine chromosome numbers and karyotypes in a few species [ 30 – 32 ]. More recently, a restriction endonuclease banding pattern was described in Ruditapes decussatus [ 33 ] and some repetitive DNA sequences were mapped by fluorescent in situ hybridization (FISH).…”

Section: Introductionmentioning

confidence: 99%

Divergent evolutionary behavior of H3 histone gene and rDNA clusters in venerid clams

et al. 2015

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BackgroundHistone H3 gene clusters have been described as highly conserved chromosomal markers in invertebrates. Surprisingly, in bivalves remarkable interspecific differences were found among the eight mussels and between the two clams in which histone H3 gene clusters have already been located. Although the family Veneridae comprises 10 % of the species of marine bivalves, their chromosomes are poorly studied. The clams belonging to this family present 2n = 38 chromosomes and similar karyotypes showing chromosome pairs gradually decreasing in length. In order to assess the evolutionary behavior of histone and rRNA multigene families in bivalves, we mapped histone H3 and ribosomal RNA probes to chromosomes of ten species of venerid clams.ResultsIn contrast with the reported conservation of histone H3 gene clusters and their intercalary location in invertebrates, these loci varied in number and were mostly subterminal in venerid clams. On the other hand, while a single 45S rDNA cluster, highly variable in location, was found in these organisms, 5S rDNA clusters showed interspecific differences in both number and location. The distribution patterns of these sequences were species-specific and mapped to different chromosomal positions in all clams but Ruditapes decussatus, in which one of the minor rDNA clusters and the major rDNA cluster co-located.ConclusionThe diversity in the distribution patterns of histone H3 gene, 5S rDNA and 28S rDNA clusters found in venerid clams, together with their different evolutionary behaviors in other invertebrate taxa, strongly suggest that the control of the spreading of these multigene families in a group of organisms relies upon a combination of evolutionary forces that operate differently depending not only on the specific multigene family but also on the particular taxa. Our data also showed that H3 histone gene and rDNA clusters are useful landmarks to integrate nex-generation sequencing (NGS) and evolutionary genomic data in non-model species.

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Cytogenetic Studies on Metaphase Chromosomes of Six Bivalve Species of Families Mytilidae and Veneridae (Nucinelloidea, Mollusca)

Cited by 6 publications

References 17 publications

Evolutionary Dynamics of rDNA Clusters in Chromosomes of Five Clam Species Belonging to the Family Veneridae (Mollusca, Bivalvia)

Evolutionary Dynamics of rDNA Clusters in Chromosomes of Five Clam Species Belonging to the Family Veneridae (Mollusca, Bivalvia)

Cytogenetic characterisation of the razor shells Ensis directus (Conrad, 1843) and E. minor (Chenu, 1843) (Mollusca: Bivalvia)

Divergent evolutionary behavior of H3 histone gene and rDNA clusters in venerid clams

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