Cytogenetic studies on human lymphoblastoid cell lines from burkitt's lymphomas and other sources

Abstract

The occurrence and incidence of a characteristic banding abnormality in a No. 14 chromosome has been studied in Burkitt-lymphoma-derived and certain other EB virus-associated lymphoblastoid cell lines. The abnormality was readily detected in 7 out of 7 Burkitt lines, and when present could be seen in 100% of cells with recognizable No. 14 chromosomes. In contrast, the abnormality was not observed in 775 cells from 31 infectious mononucleosis-derived lines nor in 450 cells from 18 lines obtained from cord blood lymphocytes experimentally transformed by EB virus in vitro. The significance of the abnormality as an indication of cells transformed by the virus in vivo is discussed, and the importance of this considered in relation to a possible oncogenic role for EB virus in man.

Cytogenetics of malignant epithelial cells and lymphoblastoid cell lines from nasopharyngeal carcinoma

“…14 chromosome of Burkitt lymphoma cells in biopsy samples and after culture (Manolov & Manolova, 1972) seems to be unrelated to EB virus, since it has been shown to be lacking in EB virus-carrying lymphoid cells from other sources (Jarvis et al, 1974;Zech et al, 1976). This concept has been confirmed by studies of somatic cell hybrids made between mouse cells and EB virus-carrying human lymphoid cells, which have shown that after the preferential loss of some human chromosomes expression of the EB virus nuclear antigen (EBNA) (Reedman and Klein, 1]973) was lost despite the continuing presence of the human D14 chromosome, indicating that the viral genome was not associated with this particular chromosome (Glaser et al, 1975).…”

“…14 chromosome abnormality in lymphoid cells of non-malignant origin, despite the presence of the EB viral genome (Jarvis et al, 1974;Zech et al, 1976), it is also not surprising that the cells of the NPC-derived lymphoblastoid lines lacked a consistent No. 14 chromosome abnormality (Table II).…”

“…The chopped tumour material in the trypsin was then gently rocked at 37°C for 30 min to give a cell suspension, after which the trypsin was inactivated by adding an equal volume of medium with 20% foetal calf serum. Dry metaphase spreads were prepared and banded from the cell suspension as in earlier work (Jarvis et al, 1974 …”

“…14 chromosome abnormality (Manolov and Manolova, 1972). In contrast EB virus-carrying lymphoblastoid lines of non-malignant origin (from blood of infectious-mononucleosis patients and normal seropositive individuals, or after in vitro transformation by the virus of lymphocytes from seronegative donors) do not possess such a chromosome change (Jarvis et al, 1974;Zech et al, 1976).…”

Epstein-Barr virus and human chromosomes: close association of the resident viral genome and the expression of the virus-determined nuclear antigen (EBNA) with the presence of chromosome 14 in human-mouse hybrid cells.

“…It has recently been reported that a translocation from chromosome 8 to the long arm of chromosome 14 is observed in lymphomas, including Burkitt tumors (18)(19)(20). Whether or not the close association of EBV genome and EBNA with chromosome 14 is related to the mechanism of EBV-induced oncogenesis is deemed worthy of investigation.…”