Cytogenetic Studies Comparing Three Characidae Fish Species from the Iguatemi River Basin, Brazil

Fernandes, Carlos Alexandre; Piscor, Diovani; Bailly, Dayani; Silva, Valéria Flávia Batista da; Martins‐Santos, Isabel Cristina

doi:10.1508/cytologia.75.329

Cited by 9 publications

(9 citation statements)

References 9 publications

(10 reference statements)

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“…These data corroborate previous information about the diploid chromosome number and karyotype characteristics reported by Hashimoto et al (2011). In contrast to the species complexes Astyanax fasciatus and Astyanax scabripinnis, which show variable diploid chromosome numbers in different individuals living in sympatry (Moreira-Filho and Bertollo 1991; Artoni, Shibatta et al 2006, Machado et al 2012, cytogenetic studies in some populations of A. bockmanni demonstrated a stable 2n (Kavalco et al 2009;Fernandes et al 2010;Hashimoto and Porto-Foresti 2010).…”

Section: Resultssupporting

confidence: 90%

“…Some studies have shown a stable diploid number of 50 chromosomes for individuals of Astyanax bockmanni. However, some populations of A. bockmanni can demonstrate a high inter-and intra-individual variability of the NOR phenotypes, as well as an extensive polymorphism in the heterochromatin distribution (Kavalco et al 2009;Fernandes et al 2010;Hashimoto and Porto-Foresti 2010).…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetic characterization of distinct B chromosomes in a population of the fish Astyanax bockmanni (Teleostei, Characiformes)

et al. 2012

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Individuals of Astyanax bockmanni from one population belonging to the Paranapanema River basin, southeastern Brazil, were cytogenetically examined. In the present paper, we report a diploid number of 50 chromosomes, with some individuals bearing additional chromosomes, characterized as different variants of B chromosomes. The B microchromosome of acrocentric morphology (B a ) was the most frequent type in this population (77.7%). Two other variants were identified as metacentric B chromosomes, but of distinct sizes, characterized as macrochromosome (B M ) and microchromosome (B m ). Conspicuous heterochromatic blocks were observed in the terminal regions of some chromosomes of the standard karyotype and partially heterochromatic blocks were detected in all B chromosomes. 18S ribosomal RNA genes were distributed in the terminal region of several chromosomes, but were not located on the B chromosomes.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Cytogenetic characterization of distinct B chromosomes in a population of the fish Astyanax bockmanni (Teleostei, Characiformes)

et al. 2012

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“…The 6 populations of A. bockmanni showed a diploid number of 2n = 50 chromosomes in all individuals analyzed, as previously observed for distinct populations of this species [Kavalco et al, 2009;Fernandes et al, 2010;Hashimoto and Porto-Foresti, 2010;Hashimoto et al, 2011;Daniel et al, 2012Silva et al, 2013]. Similar patterns were also observed in other species of the genus [Pansonato-Alves et al, 2013a;Silva et al, 2014].…”

Section: Discussionsupporting

confidence: 86%

Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes)

Daniel

Penitente²,

Silva³

et al. 2015

Cytogenet Genome Res

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An important feature of eukaryotic organisms is the number of different repetitive DNA sequences in their genome, a feature not observed in prokaryotes. These sequences are considered to be important components for understanding evolutionary mechanisms and the karyotypic differentiation processes. Thus, we aimed to physically map the histone genes and transposable elements of the Rex family in 6 fish populations of Astyanax bockmanni. FISH results using a histone H1 gene probe showed fluorescent clusters in 2 chromosome pairs in all 6 samples analyzed. In contrast, FISH with a histone H3 probe showed conspicuous blocks in 4 chromosomes in 5 of the 6 populations analyzed. The sixth population revealed 7 chromosomes marked with this probe. Probes for the transposable elements Rex1 and Rex6 showed small sites dispersed on most chromosomes of the 6 populations, and the Rex3 element is located in a big block concentrated in only 1 acrocentric chromosome of 2 populations. As for the other populations, a Rex3 probe showed large blocks in more than 1 chromosome. Fish from Alambari and Campo Novo Stream have Rex3 elements dispersed along most of the chromosomes. Additionally, the conspicuous signals of Rex1, Rex3, and Rex6 were identified in the acrocentric B microchromosome of A. bockmanni found only in individuals of the Alambari River. Thus, we believe that different mechanisms drive the spread of repetitive sequences among the populations analyzed, which appear to be organized differently in the genome of A. bockmanni. The presence of transposable elements in the B chromosome also suggests that these sequences could play a role in the origin and maintenance of the supernumerary element in the genome of this species.

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“…Cytogenetic data for the genus Bryconamericus have been described in the literature by some authors, i.e., chromosomal analyses by Giemsa, detection of major ribosomal DNA (45S), and differential staining, such as C-bands, G-bands and chromomycin A 3 (CMA 3 ) [7-10]. In these studies, the most frequently reported diploid number was 2n = 52 chromosomes; however, variation involving the chromosome morphology and the number and location of NORs were also registered.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic analysis in three Bryconamericus species (Characiformes, Characidae): first description of the 5S rDNA-bearing chromosome pairs in the genus

et al. 2013

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BackgroundNowadays, the genus Bryconamericus is placed in subfamily Stevardiinae within of Characidae, but not shows consistent evidence of monophyletism. The purpose of this work was to study the chromosomes of three species of Bryconamericus, aiming to add cytogenetic knowledge and contribute to the understanding of the chromosomal evolution of this genus.ResultsThe chromosomes of three species of Bryconamericus were analyzed using cytogenetic techniques. The karyotype of Bryconamericus stramineus contained 6 metacentric (m) + 10 submetacentric (sm) + 16 subtelocentric (st) + 20 acrocentric (a), the fundamental number (FN) of 84, one silver impregnated (Ag-NOR) pair, one pair bearing the 18S ribosomal DNA sites, another pair bearing the 5S rDNA sites, and a few positive C-bands. Bryconamericus turiuba had a karyotype containing 8 m + 10sm + 14st + 20a (FN = 84), one chromosome pair Ag-NOR, two pairs bearing the 18S rDNA sites, two pairs bearing the 5S rDNA sites, and a few C-band regions. Bryconamericus cf. iheringii had a karyotype containing 10 m + 14sm + 18st + 10a (FN = 94), including one pair with a secondary constriction Ag-NOR positive. In this karyotype the fluorescent in situ hybridization (FISH) showed the 18S and 5S rDNA probe in adjacent position.ConclusionsThe results obtained in this work showed different characteristics in the organization of two multigene families, indicating that distinct evolutionary forces acting on the diversity of rDNA sequences in the genome of three Bryconamericus species.

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Cytogenetic Studies Comparing Three Characidae Fish Species from the Iguatemi River Basin, Brazil

Cited by 9 publications

References 9 publications

Cytogenetic characterization of distinct B chromosomes in a population of the fish Astyanax bockmanni (Teleostei, Characiformes)

Cytogenetic characterization of distinct B chromosomes in a population of the fish Astyanax bockmanni (Teleostei, Characiformes)

Organization and Chromosomal Distribution of Histone Genes and Transposable<b><i> Rex</i></b> Elements in the Genome of <b><i>Astyanax bockmanni </i></b>(Teleostei, Characiformes)

Cytogenetic analysis in three Bryconamericus species (Characiformes, Characidae): first description of the 5S rDNA-bearing chromosome pairs in the genus

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