Cytogenetic characterization of distinct B chromosomes in a population of the fish Astyanax bockmanni (Teleostei, Characiformes)

Daniel, Sandro Natal; Hashimoto, Diogo Teruo; Pansonato-Alves, José Carlos; Foresti, Fausto; Porto‐Foresti, Fábio

doi:10.1080/00087114.2012.738488

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…The 6 populations of A. bockmanni showed a diploid number of 2n = 50 chromosomes in all individuals analyzed, as previously observed for distinct populations of this species [Kavalco et al, 2009;Fernandes et al, 2010;Hashimoto and Porto-Foresti, 2010;Hashimoto et al, 2011;Daniel et al, 2012Silva et al, 2013]. Similar patterns were also observed in other species of the genus [Pansonato-Alves et al, 2013a;Silva et al, 2014].…”

Section: Discussionsupporting

confidence: 70%

“…Similar patterns were also observed in other species of the genus [Pansonato-Alves et al, 2013a;Silva et al, 2014]. Additionally, individuals of the Alambari population present- ed 0-2 partially or fully heterochromatic metacentric and/or acrocentric supernumerary microchromosomes in their genome, with a morphologic behavior that was previously observed by Hashimoto et al [2011] and Daniel et al [2012. We observed the occurrence of only 4 chromosomes tagged with the probe for histone H1.…”

Section: Discussionsupporting

confidence: 67%

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Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes)

Daniel

Penitente²,

Silva³

et al. 2015

Cytogenet Genome Res

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An important feature of eukaryotic organisms is the number of different repetitive DNA sequences in their genome, a feature not observed in prokaryotes. These sequences are considered to be important components for understanding evolutionary mechanisms and the karyotypic differentiation processes. Thus, we aimed to physically map the histone genes and transposable elements of the Rex family in 6 fish populations of Astyanax bockmanni. FISH results using a histone H1 gene probe showed fluorescent clusters in 2 chromosome pairs in all 6 samples analyzed. In contrast, FISH with a histone H3 probe showed conspicuous blocks in 4 chromosomes in 5 of the 6 populations analyzed. The sixth population revealed 7 chromosomes marked with this probe. Probes for the transposable elements Rex1 and Rex6 showed small sites dispersed on most chromosomes of the 6 populations, and the Rex3 element is located in a big block concentrated in only 1 acrocentric chromosome of 2 populations. As for the other populations, a Rex3 probe showed large blocks in more than 1 chromosome. Fish from Alambari and Campo Novo Stream have Rex3 elements dispersed along most of the chromosomes. Additionally, the conspicuous signals of Rex1, Rex3, and Rex6 were identified in the acrocentric B microchromosome of A. bockmanni found only in individuals of the Alambari River. Thus, we believe that different mechanisms drive the spread of repetitive sequences among the populations analyzed, which appear to be organized differently in the genome of A. bockmanni. The presence of transposable elements in the B chromosome also suggests that these sequences could play a role in the origin and maintenance of the supernumerary element in the genome of this species.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionsupporting

confidence: 67%

Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes)

Daniel

Penitente²,

Silva³

et al. 2015

Cytogenet Genome Res

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“…Synteny between repetitive sequences can provide interesting insights into genome evolution and organization [Andrews et al, 1987;Hashimoto et al, 2012]. The proximity of histone and 5S rDNA cluster sequences in one chromosome pair of several Characidae species led some authors to suggest a possible selective advantage provided by [Hashimoto et al, 2011;Silva et al, 2013Silva et al, , 2014.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the occurrence of different types of B chromosomes in their cells, the existence of distinct karyotype formulas, and the differential distribution of repetitive DNAs in closely and distantly related species all reinforce the intense and continuous dynamics of the Astyanax genome, which includes macro-chromosomal rearrangements (e.g. fissions, fusions, and inversions) and changes at the sub-chromosomal level of resolution [Fernandes and Martins-Santos, 2005;Mantovani et al, 2005;Pazza et al, 2006;Carvalho et al, 2008;Hashimoto et al, 2008;Vicari et al, 2008;Daniel et al, 2012;Santos et al, 2013;Silva et al, 2013].…”

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confidence: 99%

Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA

Silva

Utsunomia

Pansonato-Alves

et al. 2015

Cytogenet Genome Res

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Astyanax is a genus of Characidae fishes currently composed of 155 valid species. Previous cytogenetic studies revealed high chromosomal diversification among them, and several studies have been performed using traditional cytogenetic techniques to investigate karyotypes and chromosomal locations of 18S and 5S rDNA genes. However, only a few studies are currently available about other repetitive sequences. Here, the chromosomal location of small nuclear RNA genes, identified as U1 and U2 snRNA clusters, was established and compared to the distribution of 5S rDNA and histone clusters in 5 Astyanax species (A. paranae, A. fasciatus, A. bockmanni, A. altiparanae, and A. jordani) using FISH. The cytogenetic mapping of U1 and U2 snRNA demonstrated a conserved pattern in the number of sites per genome independent of the location in Astyanax species. The location of the U1 snRNA gene was frequently associated with 5S rDNA sequences, indicating a possible interaction between the distinct repetitive DNA families. Finally, comparisons involving the location of U1 and U2 snRNA clusters in the chromosomes of Astyanax species revealed a very diverse pattern, suggesting that many rearrangements have occurred during the diversification process of this group.

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“…In Neotropical fishes, 4% of the more than 1000 species with known karyotypes have B chromosomes. 8,9 From these, seven species belong to the genus Astyanax, namely 10 A. fasciatus, A. schubarti, 11 A. altiparanae, 12 A. bockmanni, 13 A. goyacensis, 14 A. paranae, 15 and A. scabripinnis. 10,16 In A. scabripinnis, the origin of the B macrochromosomes was initially attributed to events of meiotic nondisjunction based on the comparative inferences from the size and morphology of these B chromosomes when compared to the first pair of standard A chromosomes.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Replication and Nuclear Localization of the B Chromosome in Kidney Tissue Cells in Astyanax scabripinnis (Teleostei: Characidae)

et al. 2020

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B chromosomes are extra genomic compounds found in different taxonomic groups, including plants and animals. Obtaining patterns of resolutive chromosomal bands is necessary to understand the nuclear organization, variability and nature of B chromosome chromatin and possible transcriptional regions. In this study, we analyzed 35 Astyanax scabripinnis specimens sampled from Fazenda Lavrinha, a stream in the Paraíba do Sul river basin, Brazil. Through the incorporation of the thymidine analog 5¢-bromo-2¢-deoxyuridine (5-BrdU) in vivo, it was possible to recognize the replicating regions of the B chromosome at the beginning of the S phase, differentially characterized in relationship to the regions of late replication. In this perspective, it is possible to suggest that the B chromosome of this species possesses a territory and the chromatin accessible for transcription, especially in the light (i.e., early replicating) bands (p1.1; p1.3; and p2.1 and q1.1, q1.3, q2.1, and q2.2). The latereplicating regions are corresponding to the blocks of constitutive heterochromatin. They show a preferential accumulation of satellite DNA As51. By the use of the fluorochrome chromomycin A3 (CMA3), it was possible to identify GC-rich chromosomal regions, corresponding to late-replicating parts of genome, confirming the revealed data by the replication banding and C-banding. In addition, the analysis by confocal microscopy in kidney cells indicates the location of a peripheral anchorage of this chromosome in the nuclear lamina, reinforcing the idea of downregulation of the associated regions.

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Cytogenetic characterization of distinct B chromosomes in a population of the fish Astyanax bockmanni (Teleostei, Characiformes)

Cited by 12 publications

References 25 publications

Organization and Chromosomal Distribution of Histone Genes and Transposable<b><i> Rex</i></b> Elements in the Genome of <b><i>Astyanax bockmanni </i></b>(Teleostei, Characiformes)

Organization and Chromosomal Distribution of Histone Genes and Transposable<b><i> Rex</i></b> Elements in the Genome of <b><i>Astyanax bockmanni </i></b>(Teleostei, Characiformes)

Chromosomal Mapping of Repetitive DNA Sequences in Five Species of <b><i>Astyanax</i></b> (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA

Dynamics of Replication and Nuclear Localization of the B Chromosome in Kidney Tissue Cells in Astyanax scabripinnis (Teleostei: Characidae)

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