Cytogenetic Radiosensitivity of G0-Lymphocytes of Breast and Esophageal Cancer Patients as Determined by Micronucleus Assay

Abstract

Enhanced chromosomal radiosensitivity is a feature of many cancer predisposition conditions, indicative of the important role of chromosomal alterations in carcinogenesis. In this study the cytokinesis-blocked micronucleous assay was used to compare the radiosensitivity of blood lymphocytes obtained from Iranian breast or esophageal cancer patients (n = 50, n = 16; respectively) with that of control individuals (n = 40). For each sample, one thousand binucleate lymphocytes were analyzed before and after in vitro exposure to 3 Gy of gamma rays. The radiation-induced frequency of micronucleus was significantly higher in the breast cancer group (261/1,000 binucleated cells) than in esophageal cancer group (241/1,000 binucleated cells, P < 0.01) or in the control group (240/1,000 binucleated cells, P < 0.01). The results indicate that breast cancer patients are more radiosensitive compared to normal healthy individuals or esophageal cancer patients. Increased radiosensitivity could be due to defects in DNA repair genes involved in breast cancer formation. Since patients with esophageal cancer did not show elevated radiosensitivity, it is assumed that the contribution of radiosensitivity-related genes to the development of esophageal cancer may be smaller than the contribution of those genes to breast cancer.

Effects of an Integrated Yoga Program in Modulating Psychological Stress and Radiation-Induced Genotoxic Stress in Breast Cancer Patients Undergoing Radiotherapy

“…34,35 We also reported a significant increase in radiation-induced DNA damage in breast cancer patients undergoing radiotherapy. 36 DNA damage in the form of telomere shortening has been linked to increased stress in a population of caregivers.…”

“…Radiation-induced DNA damage has been widely studied and reported by many, including our previous study. 36 In another work, Mozdarani et al 34 showed that there was an elevated spontaneous frequency of micronuclei in a breast cancer group compared to a control group. They also showed that breast cancer patients were 30% more sensitive to ionizing radiation than the age-and sex-matched control population.…”

“…There is a converging link between the psychological (QOL, anxiety, depression, mood disturbances, perceived stress ) 3,[27][28][29][30][31][32][33] and physiological stress at the molecular level such as cortisol levels, catecholamines, DNA damage, telomere length, and DNA repair capacity. [34][35][36][37][38][39][40][44][45][46] Buettner et al 7 reported in a large-scale survey of more than 2000 patients undergoing various complementary treatments that yoga was the most effective among all the CAMs in decreasing anxiety and depression and improving the QOL of breast cancer patients. In the current study, we investigated the possible link of stress at the molecular level.…”

Chromosomal radiosensitivity in head and neck cancer patients: evidence for genetic predisposition?

“…Several studies have shown that enhanced chromosomal radiosensitivity is also present in a significant proportion of cancer patients (Scott et al, 1994(Scott et al, , 1998Parshad et al, 1996;Patel et al, 1997;Terzoudi et al, 2000;Baria et al, 2001Baria et al, , 2002Buchholz and Wu, 2001;Papworth et al, 2001;Riches et al, 2001;Baeyens et al, 2002Baeyens et al, , 2005Ban et al, 2004;Howe et al, 2005;Kolusayin Ozar and Orta, 2005;Mozdarani et al, 2005;Distel et al, 2006;Lisowska et al, 2006;Varga et al, 2006). The majority of data have been collected in studies considering breast cancer patients whose lymphocytes were irradiated in the G 2 phase of the cell cycle (Scott et al, 1994Parshad et al, 1996;Patel et al, 1997;Terzoudi et al, 2000;Baria et al, 2001;Buchholz and Wu, 2001;Riches et al, 2001;Baeyens et al, 2002Baeyens et al, , 2005Howe et al, 2005;Djuzenova et al, 2006;Docherty et al, 2007).…”

Naujas požiūris į onkologinių ligonių spindulinį gydymą – individualaus radiojautrio tyrimas

“…Pagal šiuos du parametrus yra vertinamas spontaninis pažaidų dažnis, pažaidų po kraujo mėginių in vitro apšvi-tinimo 2 Gy jonizuojančiaja spinduliuote ir pažaidų po radioterapijos seansų dažnis [16,17].…”