Cytogenetic map of common bean (Phaseolus vulgaris L.)

Fonsêca, Artur; Ferreira, Joana; Santos, Tiago Ribeiro Barros dos; Mosiołek, Magdalena; Bellucci, Elisa; Kami, James; Gepts, Paul; Geffroy, Valérie; Schweizer, Dieter; Santos, Karla G. B. dos; Pedrosa‐Harand, Andrea

doi:10.1007/s10577-010-9129-8

Cited by 96 publications

(139 citation statements)

References 56 publications

(105 reference statements)

Supporting

Mentioning

130

Contrasting

Order By: Relevance

“…Most common FISH probes used in chromosome identification have been repetitive DNA elements (Mukai et al 1993;Fransz et al 1998;Lim et al 2000;Kato et al 2004;Fonseca et al 2010;Xiong et al 2011;Chester et al 2012Chester et al , 2013 or large genomic DNA clones such as BACs (Dong et al 2000;Cheng et al 2001;Kim et al 2002;Wang et al 2008;Findley et al 2010). It often takes a significant effort to develop such probes or probe sets to identify all chromosomes in each species.…”

Section: Discussionmentioning

confidence: 99%

Chromosome-Specific Painting in Cucumis Species Using Bulked Oligonucleotides

et al. 2015

View full text Add to dashboard Cite

Chromosome-specific painting is a powerful technique in molecular cytogenetic and genome research. We developed an oligonucleotide (oligo)-based chromosome painting technique in cucumber (Cucumis sativus) that will be applicable in any plant species with a sequenced genome. Oligos specific to a single chromosome of cucumber were identified using a newly developed bioinformatic pipeline and then massively synthesized de novo in parallel. The synthesized oligos were amplified and labeled with biotin or digoxigenin for use in fluorescence in situ hybridization (FISH). We developed three different probes with each containing 23,000-27,000 oligos. These probes spanned 8.3-17 Mb of DNA on targeted cucumber chromosomes and had the densities of 1.5-3.2 oligos per kilobases. These probes produced FISH signals on a single cucumber chromosome and were used to paint homeologous chromosomes in other Cucumis species diverged from cucumber for up to 12 million years. The bulked oligo probes allowed us to track a single chromosome in early stages during meiosis. We were able to precisely map the pairing between cucumber chromosome 7 and chromosome 1 of Cucumis hystrix in a F 1 hybrid. These two homeologous chromosomes paired in 71% of prophase I cells but only 25% of metaphase I cells, which may provide an explanation of the higher recombination rates compared to the chiasma frequencies between homeologous chromosomes reported in plant hybrids.

show abstract

Section: Discussionmentioning

confidence: 99%

Chromosome-Specific Painting in Cucumis Species Using Bulked Oligonucleotides

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In 2006, four BAC libraries, three for common bean genotypes BAT93, G21245, G02771, and one for lima bean, cv Henderson (P. lunatus), were developed to study the evolution of the arcelin-phytohemagglutinin-a-amylase inhibitor (APA) multigene family (Kami et al, 2006) The four BAC libraries have a range of 9-20 fold genome coverage that should make them useful genetic resources for studying common bean and lima bean. The BAT 93 BAC library has been used successfully for cytogenetic studies of bean chromosomes (Fonseca, et al 2010;Pedrosa-Harand et al 2009). BAC libraries were also developed for common bean genotypes G19833 (Schlueter et al, 2008), G12949 (Galasso et al, 2009), HR45 , G02333 (Melotto et al, 2003), HR67 and OAC-Rex .…”

Section: Genomic Mapping Of Bean With Mmsmentioning

confidence: 99%

“…BAC libraries were also developed for common bean genotypes G19833 (Schlueter et al, 2008), G12949 (Galasso et al, 2009), HR45 , G02333 (Melotto et al, 2003), HR67 and OAC-Rex . The G19833 BAC library was used for BAC end sequence analysis to develop BAC derived SSR markers and for physical mapping of the common bean genome (Fonseca, et al, 2010;Córdoba et al, 2010). Liu et al (2010) used the HR45 BAC library to physically map the major CBB resistance QTL of common bean to the end of chromosome 6.…”

Section: Genomic Mapping Of Bean With Mmsmentioning

confidence: 99%

“…The standard nomenclature can be found in the BIC website (http://www.css.msu.edu/bic/Genetics.cfm). In two recent studies, a cytogenetic map of common bean was built by FISH of BACs selected with markers mapping to the 11 linkage groups, plus 2 plasmids for 5S and 45S ribosomal DNA and one bacteriophage (Pedrosa et al, 2009;Fonseca et al, 2010). It was found that about 50% of the bean genome is heterochromatic and that genes and repetitive sequences on the bean chromosomes are intermingled in the euchromation and heterochromatin.…”

Section: Genomic Mapping Of Bean With Mmsmentioning

confidence: 99%

See 1 more Smart Citation

Development and Application of Molecular Markers to Breed Common Bean (Phaseolus vulgaris L.) for Resistance to Common Bacterial Blight (CBB) — Current Status and Future Directions

Yu¹,

Shi²,

Zhang³

2012

Applied Photosynthesis

View full text Add to dashboard Cite

“…Karyotype analysis to differentiate species within Phaseolus revealed variations within the genus, and there are reports of species with x = 10 (Mercado-Ruaro and Delgado-Salinas, 1998, 2009. Chromosomes are small (about 2 mm) and have similar morphologies (Fonsêca et al, 2010). Available data show that there is a predominance of metacentric and submetacentric chromosomes, which result in very symmetrical karyotypes.…”

Section: Introductionmentioning

confidence: 99%

Implications of mitotic and meiotic irregularities in common beans (Phaseolus vulgaris L.)

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. The common bean has great social and economic importance in Brazil and is the subject of a high number of publications, especially in the fields of genetics and breeding. Breeding programs aim to increase grain yield; however, mitosis and meiosis represent under explored research areas that have a direct impact on grain yield. Therefore, the study of cell division could be another tool available to bean geneticists and breeders. The aim of this study was to investigate irregularities occurring during the cell cycle and meiosis in common bean. The common bean cultivar used was BRSMG Talismã, which owing to its high yield and grain quality is recommended for cultivation in Brazil. We classified the interphase nuclei, estimated the mitotic and meiotic index, grain pollen viability, and percentage of abnormalities in both processes. The mitotic index was 4.1%, the interphase nucleus was non-reticulated, and 19% of dividing somatic cells showed abnormal behavior. Meiosis also presented irregularities resulting in a meiotic index of 44.6%. Viability of pollen grains was 94.3%. These results indicate that the common bean cultivar BRSMG Talismã possesses repair mechanisms that compensate for changes by producing a large number of pollen grains. Another important strategy adopted by bean plants to ensure stability is the elimination of abnormal cells by apoptosis. As the common bean cultivar BRSMG Talismã is recommended for cultivation because of its good agronomic performance, it can be concluded that mitotic and meiotic irregularities have no negative influence on its grain quality and yield.

show abstract

Cytogenetic map of common bean (Phaseolus vulgaris L.)

Cited by 96 publications

References 56 publications

Chromosome-Specific Painting in Cucumis Species Using Bulked Oligonucleotides

Chromosome-Specific Painting in Cucumis Species Using Bulked Oligonucleotides

Development and Application of Molecular Markers to Breed Common Bean (Phaseolus vulgaris L.) for Resistance to Common Bacterial Blight (CBB) — Current Status and Future Directions

Implications of mitotic and meiotic irregularities in common beans (Phaseolus vulgaris L.)

Contact Info

Product

Resources

About