Development and Application of Molecular Markers to Breed Common Bean (Phaseolus vulgaris L.) for Resistance to Common Bacterial Blight (CBB) — Current Status and Future Directions

Yu, Kangfu; Shi, Chun; Zhang, Bailing

doi:10.5772/26707

Cited by 6 publications

(12 citation statements)

References 92 publications

(112 reference statements)

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“…Common bacterial blight is the most destructive bacterial disease of common bean in Uganda, and can cause up to 62% yield losses (Opio and Namayanja, 2002). Being seed-borne, it reduces seed quality through staining and browning (Yu et al, 2012), and constitutes a real threat to seed production. It is generally endemic in bean growing areas under high temperature, rainfall, and relative humidity (Saettler, 1991).…”

Section: Introductionmentioning

confidence: 99%

Identification of common bean genotypes with dual leaf and pod resistance to common bacterial blight disease in Uganda

Alladassi¹,

Nkalubo²,

Mukankusi³

et al. 2018

Afr. Crop Sci. J.

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Common Bacterial Blight (CBB), caused by Xanthomonas axonopodis pv. phaseoli (Xap) and Xanthomonas axonopodis pv. phaseoli var. fuscans (Xapf), is a serious disease that affects common bean production worldwide. In Uganda, it is the most severe and widely occurring bacterial disease, causing significant yield losses in common bean. Although various sources of resistance have been developed around the world, none of the varieties grown in Uganda is known to be resistant. The objective of this study was to identify lines with combined leaf and pod resistance for introgression into locally adapted but susceptible Ugandan genotypes. A total of 132 common bean accessions was evaluated in a screenhouse and under field conditions, in an 11x12 alpha lattice design. Genotypes were inoculated with a local isolate in a screenhouse; while the plants were left to undergo natural infestation in field. Results indicated significant differences (P<0.001) in genotypic reactions against the CBB disease, with a range of disease scores of 2.2-7.8 on leaves and 2.6-7.1 on pods (1-9 CIAT disease scale), suggesting high genetic variability among the tested germplasm. Relatively low correlation (r = 0.39) was observed between leaf and pod reactions, suggesting differential expression of CBB resistance in these two plant organs. Overall, four genotypes, NE2-14-8, NE17-14-29, NE14-09-78 and VAX3, consistently showed resistance in both screenhouse and field evaluations, leaf and pod inoculations and at all sampling stages. These genotypes were, therefore, identified for transferring CBB resistance into Ugandan susceptible market class bean varieties.

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Section: Introductionmentioning

confidence: 99%

Identification of common bean genotypes with dual leaf and pod resistance to common bacterial blight disease in Uganda

Alladassi¹,

Nkalubo²,

Mukankusi³

et al. 2018

Afr. Crop Sci. J.

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“…For example, the great northern common bean landrace Montana No. 5 [12,18,[59][60][61] and inter-specific breeding lines XAN and VAX were used to develop CBB resistance [24,32,34]. Notably, tepary beans (Phaseolus acutifolius, Gray) lines PI319443 and PI440795 have been used to develop CBB-resistant genotypes by most of the breeding programs in North America [18,23].…”

Section: Discussionmentioning

confidence: 99%

Sources of Resistance to Common Bacterial Blight and Charcoal Rot Disease for the Production of Mesoamerican Common Beans in the Southern United States

Ambachew

Joshua

Mmbaga

et al. 2021

Plants

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The gene pool of Mesoamerican common beans (Phaseolus vulgaris L.) includes genotypes in the small-to-medium-size seeded dry beans, as well as some snap beans from hotter environments adapted to the Southeastern United States. However, the warm and humid climate of the Southeastern United States is conducive to diseases such as Common Bacterial Blight (CBB) and Charcoal Rot (CR). The pathogens for these two diseases can survive long periods in infested soil or on seeds and are difficult to control through pesticides. Hence, field-level resistance would be the best management strategy for these diseases. The goals of this study were (1) to evaluate field-level resistance from the various commercial classes and subgroups represented in the Mesoamerican gene pool as sources for breeding beans for the region and (2) to evaluate genome-wide marker × trait associations (GWAS) using genetic markers for the genotypes. A total of 300 genotypes from the Mesoamerican Diversity Panel (MDP) were evaluated for CBB and CR in field experiments for three years. CBB resistance was also tested with a field isolate in controlled greenhouse conditions. The analysis of variance revealed the presence of variability in the MDP for the evaluated traits. We also identified adapted common bean genotypes that could be used directly in Southeastern production or that could be good parents in breeding programs for CBB and CR resistance. The GWAS detected 14 significant Single-Nucleotide Polymorphism (SNP) markers associated with CBB resistance distributed on five chromosomes, namely Pv02, Pv04, Pv08, Pv10, and Pv11, but no loci for resistance to CR. A total of 89 candidate genes were identified in close vicinity (±100 kb) to the significant CBB markers, some of which could be directly or indirectly involved in plant defense to diseases. These results provide a basis to further understand the complex inheritance of CBB resistance in Mesoamerican common beans and show that this biotic stress is unrelated to CR resistance, which was evident during a drought period. Genotypes with good yield potential for the Southeastern U.S. growing conditions were found with resistant to infection by the two diseases, as well as adaptation to the hot and humid conditions punctuated by droughts found in this region.

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“…The symptoms are found on leaves, having leaf spots which further coalesce causing leaf blight and also invade vascular tissues and infects pods, seeds and stems. The breeding for resistance is difficult due to identification of 24 QTLs that are distributed among all 11 chromosomes or linkage groups, and the influence of environment expresses these QTLs, plant maturity, disease pressure and infected organs of the plant, making this process more complex (Yu et al 2012). The source of significant QTLs or resistance gene is tepary bean cultivars viz., PI319443, G40001 and PI 44079.…”

Section: Fungal Diseasesmentioning

confidence: 99%

Revisiting and Enlisting Important QTLs Identified in Frech Bean (<em>Phaseolus vulgaris</em> L.): A Review

Dhaliwal¹,

Salaria²,

Kaushik³

2020

Preprint

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French bean (Phaseolus vulgaris L.) a member of family Leguminosae is a useful source of protein (&sim;22%), minerals (folate), vitamins and fibre. Abiotic and biotic stresses are the constraints to high yield and production of French bean. Varieties reluctant to diseases as well as abiotic stresses is among the top breeding objectives for the French bean. Mendelian ratios could know the genetically reliable forms of resistance, whereas it's more robust to understand the intricate kinds, often referred to as quantitative trait loci (QTL). Here, we review and compile the information from the studies related to the identification of QTLs for critical biofortification traits, biotic and abiotic stresses in French bean. Successful map-based cloning requires QTLs represent single genes which could be isolated in near-isogenic lines, and also the genotypes could be unambiguously inferred by progeny testing. Overall, this information will be useful for directing the French bean breeders to select a suitable method for the inheritance evaluation of quantitative traits and determining the novel genes in germplasm resources to ensure that much more potential of genetic information may be uncovered.

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Development and Application of Molecular Markers to Breed Common Bean (Phaseolus vulgaris L.) for Resistance to Common Bacterial Blight (CBB) — Current Status and Future Directions

Cited by 6 publications

References 92 publications

Identification of common bean genotypes with dual leaf and pod resistance to common bacterial blight disease in Uganda

Identification of common bean genotypes with dual leaf and pod resistance to common bacterial blight disease in Uganda

Sources of Resistance to Common Bacterial Blight and Charcoal Rot Disease for the Production of Mesoamerican Common Beans in the Southern United States

Revisiting and Enlisting Important QTLs Identified in Frech Bean (<em>Phaseolus vulgaris</em> L.): A Review

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