Cytogenetic impact of sodium chloride stress on root cells of Vigna radiata L. seedlings

Farheen, Jabeen; Mansoor, Simeen

doi:10.1515/tjb-2018-0352

Cited by 3 publications

(5 citation statements)

References 19 publications

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“…Twenty healthy seeds were imbibed separately in pretreatment solution of 0.01 mM Cd, Ni, Hg, Pb, Cu and distilled water (control) for 24 h, allowed to germinate in Petri dishes with distilled water for 24 h. Later treated with five respective metals of 0.1 mM and 0.5 mM concentration for 5 h. Root tips were collected for cytological analysis. Root tips were washed with distilled water thrice and fixed in Farmer s fixative solution (3 : 1 absolute alcohol: glacial acetic acid) for 24 h, later stained in 1.8% aceto-orcein according to Fiskesjo (1997) with minor modifications (Farheen and Mansoor 2019). Stained was prepared by mixing 1.8 g of aceto-orcein in 100 mL of 45% glacial acetic acid followed by boiling in a water bath for 7 h on a hot plate (M6).…”

Section: Methodsmentioning

confidence: 99%

Genotoxicity of Heavy Metals on Mung Bean (<i>Vigna radiata</i>) Seedlings and Its Alleviation by Priming with Their Lower Concentrations

et al. 2020

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Due to urbanization and industrialization, heavy metals have become the common environmental pollutant throughout the world. Chromosomal studies are one of the important tools to estimate the genome damaging property of these metals. This research was done to analyze the genotoxic effects of metals such as cadmium (Cd), nickel (Ni), mercury (Hg), lead (Pb) and copper (Cu) in mung bean (Vigna radiata) root tips. The experiment was set in such a way that mild metal treatment (0.01 mM) was given before respective high metal treatments (0.1 and 0.5 mM) separately, with the aim to know whether low/mild metals can alleviate metal tolerance in mung bean seedlings. Results demonstrated that metals treatment resulted in growth and mitotic index reduction. Among all the tested metals, Cd was found to cause more negative effect on growth of mung bean seedlings with length of 6.12 cm and 13.41% mitotic Index (MI) with the highest total aberrations (27.91%) at 0.5 mM Cd. Positive correlation was found between mitotic index and seedling length while a negative correlation was recorded between chromosomal aberrations with mitotic index and seedling length. Among these abnormalities C-mitosis, sticky chromosomes, and disturb metaphase were the most common aberrations irrespective of the metal. Furthermore, it was also observed that seed priming with respective mild metal concentration improves the seedling length, MI, and reduces the effect of metal stress by lowering the chromosomal aberrations in both 0.1 mM and 0.5 mM concentrations as compared to metal stress alone.

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Section: Methodsmentioning

confidence: 99%

Genotoxicity of Heavy Metals on Mung Bean (<i>Vigna radiata</i>) Seedlings and Its Alleviation by Priming with Their Lower Concentrations

et al. 2020

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“…The application of SA had a positive effect on the protein content under salt stress. [109,111,131]. reported an increase in protein content in saltstressed plants when applying SA.…”

Section: Discussionmentioning

confidence: 99%

Salicylic Acid Alleviates the Adverse of Salinity Stress in Fenugreek (Trigonella foenum-graecum)

AL-Maqtary,

AL-Madhagi,

AL-Mureish

2024

AJOB

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Aims: To discover the resistance of the Yemeni genotype of fenugreek to salt stress and the impact of salicylic acid on alleviating salt stress. Study Design: The experimental design adopted a completely randomized block design (RCBD), with pots filled with a 1:2 mixture of sand and soil serving as the experimental units. Place and Duration of Study: in a mini greenhouse covered with white polyethylene plastic sheet at Taiz University during the spring season 2021. The average temperature ranged from 32 ℃ during the day to 29 ℃ at night. chemical analyses were performed at the pharmaceutical laboratory in Al-Saeed University. Methodology: The fenugreek genotype was subjected to varying salinity levels (0, 50, 100, 150, and 200 mM) and the impact of salicylic acid (at concentrations of 0, 0.1, and 0.5 mg/l) in alleviating salt stress effects was examined at two time points: 15 and 30 days after 2 weeks of germination. Results: Increasing salinity significantly reduced shoot and root length and exhibited slight fluctuations influenced by salinity levels above 100 mM. Salicylic acid (SA) significantly reduced shoot length but had no effect on root length. Salinity had no appreciable effect on shoot dry matter (SDM%), while the application of SA significantly decreased it. Root dry matter percentage (RDM%) showed a notable and sharp increase with salinity. However, no appreciable changes in RDM% were observed in response to the SA application. In response to salinity, the ratio of SDM% to RDM% in treated plants decreased sharply, with the lowest ratio achieved when 0.1 SA was used for treatment. There were no significant modifications observed in the amounts of photosynthetic pigments in fenugreek leaves, including chl a, chl b and total chl. Nevertheless, using high concentration of Nacl led to a signifcantly reduced in the carotenoids and the chl. b. The protein content of fenugreek plants treated with salt was much higher than that of control plants. Also, protein levels increased with SA treatment across all NaCl concentration. Proline levels sharply increased with rising salinity, reaching a peak in approximately 22.28 ug/g DW. Application of 0.1 SA led to the highest proline level in comparison to other treatments, which was the highest level of Nacl in the second measurement time. Proline levels in plants treated with 200 Nacl and 0.1 SA were the highest, averaging roughly recorded 49.95 ug/g DW. APX significantly affected on fenugreek plants, the highest APX content was found in plants treated with 50 mM NaCl, the application of 0.1 SA produced the highest level of APX (0.373nm/g protein/minuet) in fenugreek. Catalase (CA) levels significantly decreased, but SA application did not affect CA levels. The data obtained may help in determining fenugreek cultivation areas and in managing breeding programs.

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“…While food colour additives were group-2 that was first placed in the distilled water filled beaker until the adventitious root of onion bulbs became 1-2 cm long. Then onion bulbs were transferred into the separate beakers having different colour additives for 36 h. After colour additives treatment, onion root tips were cut and preserved in "Farmer Fixative" for 24 h (Farheen and Mansoor, 2019). The onion root cells were hydrolyzed in the 1N hydrochloric acid solution for 5 minutes at 60 °C then stained in 1.8% aceto-orcein for 24 h (Farheen and Mansoor, 2019).…”

Section: Microscopic Methodsmentioning

confidence: 99%

“…Then onion bulbs were transferred into the separate beakers having different colour additives for 36 h. After colour additives treatment, onion root tips were cut and preserved in "Farmer Fixative" for 24 h (Farheen and Mansoor, 2019). The onion root cells were hydrolyzed in the 1N hydrochloric acid solution for 5 minutes at 60 °C then stained in 1.8% aceto-orcein for 24 h (Farheen and Mansoor, 2019). The mitotic index and chromosomal aberrations were captured by Nikon DS-F1 Japan at 100x objective lens and computed via formulae (Farheen and Mansoor, 2019).…”

Section: Microscopic Methodsmentioning

confidence: 99%

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Geno-Toxic Appraisal of Widely Used Food Color Additives on Model Plant Allium cepa Root Tip Cells

Farheen¹,

Mansoor²,

Abid³

2021

JIS

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Azo dyes are consisted of nitrogen and carbon atom having R-N= N-R' functional group in which Rs is usually aryl. The delocalization of aryl gives vivid colors such as red, orange, and yellow. It is usually produced by an electrophilic substitution reaction of an aryl diazotization with acetoacetic amide or aromatic amines (Clarke and Kirner, 1941), which are well documented carcinogenic and mutagenic agents (Khan et al., 2020).Several studies have been conducted on the nature and characterization of azo dyes which presented a higher level of cytotoxicity on plants cells, severe allergic reactions in humans, and even cancer in lab-tested animals than any other synthetic FCAs (Rangan and Barcelous, 2009;Micic et al., 2014;Kaur et al., Abstract | The frequent use of meal and beverages having food color additives (FCAs) may cause mutation in humans' proto-oncogenes which leads to carcinogenicity. The current findings aimed to evaluate the genotoxic impact of widely used azo FCAs on the cell cycle by using onion as a model plant. The study was designed in a complete randomized design where the grown onion roots were exposed to 0%, 0.001%, 0.01%, 0.1%, and 1% concentration of FCA for 120 hours for macroscopic and 36 h for microscopic evaluation. The onion root tips morpho-physiology was severely affected as the concentration of FCAs increased. The macroscopic analysis manifested that allura red had 95% broken-ended extremely thin and transparent root tips at 1% concentration. In the microscopic analysis, FCAs induced eight different kinds of chromosomal aberrations in which allura red significantly induced the highest frequencies of abnormal prophase, sticky metaphase, c-mitosis, sticky anaphase, and laggard anaphase at each concentration. While, dislocated metaphase, forwarded anaphase, and bridge anaphase were only found in sunset yellow, tartrazine, and fast green, respectively. The use of evaluated FCAs especially allura red in foodstuff, cosmetics, and skincare products can cause cancer in humans.

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Cytogenetic impact of sodium chloride stress on root cells of Vigna radiata L. seedlings

Cited by 3 publications

References 19 publications

Genotoxicity of Heavy Metals on Mung Bean (<i>Vigna radiata</i>) Seedlings and Its Alleviation by Priming with Their Lower Concentrations

Genotoxicity of Heavy Metals on Mung Bean (<i>Vigna radiata</i>) Seedlings and Its Alleviation by Priming with Their Lower Concentrations

Salicylic Acid Alleviates the Adverse of Salinity Stress in Fenugreek (Trigonella foenum-graecum)

Geno-Toxic Appraisal of Widely Used Food Color Additives on Model Plant Allium cepa Root Tip Cells

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