Cytogenetic findings on six posterior uveal melanomas. Involvement of chromosomes. 3, 6 and 8.

Sisley, Karen; Rennie, IG; Cottom, Dennis; Potter, A. M.; Potter, C. W.; Rees, RC

doi:10.1016/0165-4608(91)90469-b

Cited by 11 publications

(15 citation statements)

References 3 publications

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“…Recurrent chromosomal abnormalities affecting chromosome 1, 3, 6 and 8 have been described and correlated with prognosis [7,19]. Loss of chromosome 3, loss of short arm of chromosome 1 and gain of 8q have been associated with decreased survival [10,11,14,15]. Because histologic material is usually not available when conservative treatment is used in the management of posterior uveal melanoma, conservatively treated patients have no complete information about life prognosis [6,9,11].…”

Section: Discussionmentioning

confidence: 99%

“…Some retrospective analyses of cytogenetic data have hypothesized that two cytogenetic pathways of clonal evolution exist in uveal melanoma: one starts with the loss of an entire chromosome 3 and continues with gains of 8q; the second pathway starts with gain of 6p [9][10][11]. Longterm studies have shown that about 70% of patients with monosomy 3 in the primary tumor died from metastases within 4 years after the initial diagnosis, whereas tumors with normal chromosome 3 status (disomy 3) rarely gave rise to metastatic disease [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vivo monosomy 3 detection of posterior uveal melanoma: 3-year follow-up

Midena

Bonaldi

Parrozzani

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vivo monosomy 3 detection of posterior uveal melanoma: 3-year follow-up

Midena

Bonaldi

Parrozzani

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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“…Previous cytogenetic studies have revealed non-random abnormalities of chromosomes 1, 3, 6 and 8. [3][4][5] Cytogenetic abnormalities of primary uveal melanomas are relatively well characterised and for some changes, clear relationships to tumour phenotype have been made. Loss of chromosome 3 is associated with a poor prognosis [6], whilst gain of chromosome 8q, frequently occurring in combination with loss of chromosome 3, also confers a worse prognosis [5,7,8].…”

Section: Introductionmentioning

confidence: 99%

The identification of chromosome abnormalities associated with the invasive phenotype of uveal melanoma in vitro

Cross¹,

Rennie²,

Murray³

et al. 2005

Clin Exp Metastasis

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Tumour cell cultures are often highly heterogeneous, containing sub-populations of cells with differing characteristics. To identify chromosome abnormalities that are associated with the invasive phenotype, we isolated highly invasive uveal melanoma cell populations using the Transwell assay. Using this invasion assay, invasive sub-populations of primary uveal melanoma short-term cultures, and an established cell line, were specifically isolated. A series of sequential assays were undertaken to enrich the invasive population, and the enhanced invasive ability was confirmed by Transwell invasion assay. Chromosome abnormalities in invasive and parental cells were identified by karyotyping and confirmed by comparative genome hybridisation. Invasive sub-populations of uveal melanoma cells were isolated from 3 uveal melanoma short term cultures and a uveal melanoma cell line. In all cases, invasive sub-populations had either acquired additional chromosome abnormalities to those present in the parental cell line, or other abnormalities present in the parental lines were lost. In the established cell line (SOM 157), invasive cells were characterised by widespread chromosomal instability, frequent telomere associations and additional copies of chromosome 20. The invasive phenotype of SOM 196 associated with the presence of a derivative chromosome 5, der(5)t(5;11)(q35;q12) whilst a translocation t(17;20)(q12;q13) was predominant amongst non-invasive cells. In two additional cultures, deletions on chromosome 6q were associated with reduced invasive ability. In conclusion, highly invasive populations of uveal melanoma cells demonstrate chromosomal abnormalities that differ from non-invasive cells. These include chromosome instability and abnormalities of chromosome 20, observations echoing those seen in metastatic uveal melanoma.

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“…Cytogenetic studies have identified a number of chromosomal abnormalities in uveal melanoma, of which loss of chromosome 1p, gain of 6p and i8q, and loss of one copy of chromosome 3 (i.e., monosomy 3) commonly occur. [3][4][5][6][7] Monosomy 3 is highly correlated with decreased survival and metastatic disease, 8,9 suggesting the presence of a tumor suppressor gene (TSG) at this location. Despite this information, genetic analysis of this chromosome has not revealed specific TSGs as players in uveal melanoma pathogenesis.…”

mentioning

confidence: 99%