In vivo monosomy 3 detection of posterior uveal melanoma: 3-year follow-up

Midena, Edoardo; Bonaldi, Laura; Parrozzani, Raffaele; Radin, P.; Boccassini, Barbara; Vujosevic, Stela

doi:10.1007/s00417-007-0692-4

Cited by 34 publications

(46 citation statements)

References 32 publications

(63 reference statements)

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“…Unfortunately, none of the cases that yielded an insufficient aspirate came to enucleation so we cannot confirm this theory. As mentioned in our Introduction, failure of FNAB to yield a sufficiently cellular specimen of a presumed choroidal or ciliary body melanoma for cytopathologic classification is a problem encountered in many reported series (8,10,(21)(22)(23)(24)(25)(26) . In the various reported series, the principal factors associated with such insufficiency have been limited tumor thickness (22) , the differential diagnostic subcategory of the tumor (i.e., "unequivocal melanoma" versus "atypical but probable melanoma" versus "nevus versus melanoma") (45) , and the intention category of the biopsy (i.e., diagnostic, investigational, prognostic).…”

Section: Discussionmentioning

confidence: 99%

“…Over the years, various authors have described and illustrated the cytomorphological and immunocytochemical features of melanocytic uveal tumor cells obtained by FNAB (1,13,(15)(16)(17)(18)(19)(20)(21) and the relative frequencies of spindle, epithelioid, mixed and necrotic tumor cells in the obtained specimens (13,15,17) . A few groups of investigators have described the correlation between cytopathological and histopathological classification of the uveal me lanoma cells in cases treated by enucleation or transcleral tumor resection, (4,10,15,17,22) and some investigators have also reported the frequency of insufficient aspirates for cytopathological diagnosis in their series and factors associated with this result (3,8,10,(21)(22)(23)(24)(25)(26)(27) . .…”

Section: Introductionmentioning

confidence: 99%

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Prognostic implications of cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy

Augsburger¹,

Corrêa²,

Trichopoulos³

2013

Arq. Bras. Oftalmol.

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Purpose: Determine whether cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy (FNAB) is a significant prognostic factor for death from metastasis. Methods: Retrospective analysis of cases of clinically diagnosed uveal melanoma evaluated by fine-needle aspiration biopsy from 1980 to 2006. Main outcome evaluated was death from metastasis. Associations between baseline clinical variables and cytopathologic classification were evaluated using cross-tabulation. Prognostic significance of cytopathologic classification was evaluated by Ka planMeier and Cox proportional hazards analysis. Results: Of 302 studied biopsies, 260 (86.1%) yielded sufficient cells for cytopathologic classification. Eighty of the 260 patients who had a sufficient specimen have already died (P=0.021), 69 from metastatic uveal melanoma. Cell type assigned by cytopathology was strongly associated with metastasis/metastatic death in this series (P=0.0048). Multivariate analysis showed cytopathologic classification to be an independently significant prognostic factor for metastatic death (P=0.0006). None of the 42 patients whose tumor yielded insufficient aspirates (sampled in at least two sites) have developed metastasis or died of metastasis thus far. Conclusion: In this series, cytopathology of fine-needle aspiration biopsy samples obtained from uveal melanomas was strongly prognostic of death from metastasis. Insufficiently aspirates (2 or more sites sampled) proved to be prognostic of a favorable outcome (i.e., not developing metastasis). Keywords

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prognostic implications of cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy

Augsburger¹,

Corrêa²,

Trichopoulos³

2013

Arq. Bras. Oftalmol.

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“…An important finding is that karyotyping and FISH especially fail quite often in previously irradiated tumors, supporting the approach in which biopsies are taken prior to irradiation. [37][38][39] However, as more chromosome aberrations are observed after prior irradiation, one may still wish to determine the chromosome status in postirradiation enucleated eyes. It will be interesting to see how irradiation affects the outcomes of other DNA-based tests such as MLPA, SNP, or droplet digital PCR or any RNA-based techniques such as class I/class II testing in enucleated eyes.…”

Section: Discussionmentioning

confidence: 99%

Radiation Treatment Affects Chromosome Testing in Uveal Melanoma

Doğrusöz

Kroes

Duinen

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. The purpose of this study was to determine whether radiation treatment induces chromosomal aberrations in uveal melanoma (UM) and to evaluate which tumor features determine success of karyotyping and FISH. METHODS.Material from 327 UM-containing enucleated eyes was submitted for karyotyping, while FISH for chromosome 3 was performed in 248 samples. Thirty-six UMs had previously undergone irradiation. Karyotypes were analyzed, and the success rate of karyotyping/FISH was evaluated and compared with clinicopathologic tumor characteristics and prior irradiation.RESULTS. Aberrations were observed in all chromosomes, with chromosomes 1, 3,6,8,13,15,16, and Y being altered in at least 15% of the tumors. Aberrations were more common and more complex in previously irradiated tumors (significant for chromosomes 5 [P ¼ 0.004] and 13 [P ¼ 0.04]). Karyotyping and FISH failed significantly more often in irradiated tumors (both P < 0.001). In nonirradiated cases, successful karyotyping was related to a large tumor prominence (P ¼ 0.004) and a high mitotic count (P ¼ 0.007). The success of FISH in these tumors was not associated with any of the studied parameters. In irradiated tumors, karyotyping succeeded more frequently in cases with a high mitotic count (P ¼ 0.03), whereas FISH was more often successful in tumors with a high mitotic count (P ¼ 0.001), a large diameter (P ¼ 0.009) and large prominence (P ¼ 0.008).CONCLUSIONS. Karyotyping and FISH are more often successful in UMs with features characteristic of high tumor aggressiveness, whereas prior irradiation leads to multiple chromosome aberrations and to unsuccessful tests. It will be interesting to determine whether other techniques can provide reliable information on the chromosome status of previously irradiated UMs.

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“…However, because of sampling difficulties, the accuracy of diagnosis in presumed uveal melanoma of transscleral FNAB ranges from only 71% (Young et al 2008) to 77% (Midena et al 2007), whereas that of transvitreal FNAB is 97% (Shields et al 2007). It appears that accuracy improves to 91% when transscleral FNAB is restricted to anteriorly located lesions of the ciliary body (Midena et al 2007). A transvitreal route using a 25-gauge vitreous cutter allows a larger tissue sample to be extracted without causing significantly more complications.…”

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confidence: 99%