Cytogenetic Collection of Uzbekistan

Sanamyan, M. F.; Petlyakova, J.E.; Rakhmatullina, E. M.; Sharipova, Elnora A.

doi:10.5772/58589

Cited by 8 publications

(8 citation statements)

References 42 publications

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“…The research material was hybrid monosomic cotton plants obtained by crossing monosomic lines of different origin from the cytogenetic cotton collection G. hirsutum of the National University of Uzbekistan (NUUz) (Sanamyan et al, 2014), with translocation lines with identified chromosomes of the American cytogenetic collection (Stelly, 1993). Monosomic cotton lines grow year-round in the cellophane envelopy greenhouse of NUUz, which are monitored and all agricultural activities are carried out.…”

Section: Methodsmentioning

confidence: 99%

“…For many years, studies on the induction of cotton plants by chromosomal aberrations using various methods of induced mutagenesis have been conducted at the National University of Uzbekistan (Sanamyan, 2003, Samanyan, Rakhmatullina, 2003. As a result, a unique cytogenetic collection of cotton was created, including monosomic, monotelodisomic, and translocation lines, which place second in the world in terms of the number of lines after a similar collection created in the USA (Sanamyan et al, 2010(Sanamyan et al, , 2014.…”

Section: Introductionmentioning

confidence: 99%

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Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Sanamyan

Bobokhujayev

2019

Vestn. VOGiS

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The lack of clear morphological markers of cotton chromosomes contributed to the development of an unconventional method for marking chromosomes using translocations. Today, tester translocation cotton lines represent the most complete set of cytological markers. The results of cytogenetic analysis of F1 hybrids obtained from crosses of monosomic cotton lines with translocation lines with identified chromosomes are presented. Cytogenetic identification and numbering of univalent chromosomes in 25 monosomic lines of the cytogenetic collection of the National University of Uzbekistan allowed us to establish the following univalent occurrences: chromosome 2 in four monosomic lines, chromosome 4 in 15 lines, chromosome 6 in four lines, chromosome 7 of the At-subgenome in one line and chromosome 18 of the Dt-subgenome in one line. The remaining 21 lines were duplicates of three non-homologous chromosomes. All monosomic lines identified were characterized by differences in univalent sizes, meiotic index, number of tetrads with micronuclei, pollen fertility, frequency of monosomy in the progeny, and a complex of morphological characters associated with the monosomy of the chromosome identified. Despite differences in the genotypic environment and methods for producing monosomics in the two cotton collections, there is a surprising coincidence of data suggesting a higher frequency of chromosomes 2, 4 and 6 occurring as monosomics, while the other chromosomes of the set occur as monosomics at a much lower frequency, and eight nonhomologous chromosomes (5, 8, 13 of the At-subgenome and 14, 15, 19, 22 and 24 of the Dt-subgenome of cotton) never do.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Sanamyan

Bobokhujayev

2019

Vestn. VOGiS

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Section: Introductionmentioning

confidence: 99%

“…Previously, we obtained new monosomic cotton lines in a single genotypic background of the highly inbred G. hirsutum L. line L-458 (A t D t genome) [27][28][29][30]; these lines resulted in long-term inbreeding and selection with the cotton cultivar 108-F. Univalent chromosomes of these lines were identified using a well-defined tester set of cotton translocation lines (USA) [31,32]. The identification of most monosomic lines was confirmed using SSR markers at the Center for Genomics and Bioinformatics of the Academy of Sciences of the Republic of Uzbekistan [30,31]. As a result, four monosomic lines from the Cytogenetic Collection of Uzbekistan (Mo11, Mo16, Mo19, and Mo93) were found to have chromosome 2 of the A t subgenome; 16 monosomic lines (Mo7, Mo31, Mo38, Mo58, Mo59, Mo60, Mo69, Mo70, Mo71, Mo72, Mo73, Mo75, Mo76, Mo79, Mo81, and Mo89) had chromosome 4 of the A t subgenome; five monosomic lines (Mo13, Mo34, Mo67, Mo92, and Mo95) had chromosome 6 of the A t subgenome; Mo27 had chromosome 7 of the A t subgenome; Mo48 line had chromosome 18 of the D t subgenome; and monotelodisomic line Mo21 lacks an arm of chromosome 11 of the A t subgenome [31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Features of Chromosome Introgression from Gossypium barbadense L. into G. hirsutum L. during the Development of Alien Substitution Lines

Sanamyan

Bobohujayev

Abdukarimov³

et al. 2022

Plants

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The creation of G. barbadense L./G. hirsutum L. chromosome-substitution linesis an important method to transfer agronomically valuable traits from G. barbadense into G. hirsutum. In this study, 30 monosomic lines of G. hirsutum from the Cytogenetic Collection of Uzbekistan, created in the genotypic background of line L-458, were used in crosses with the G. barbadense line Pima 3-79 to create substitution lines. In the course of this work, new monosomic lines were identified for chromosome 12 and monotelodisome 6 of the Atsubgenome and for chromosomes 17, 21, and 22 of the Dtsubgenome using chromosome-specific SSR markers and a well-defined tester set of cotton translocation lines (USA). Compared to those in the F1 hybrids, a strong decrease in the crossing and setting rates was found in the BC1F1 backcross lines, with the substitution of chromosomes 2, 4, 6, 7, and 12 of the Atsubgenome and 17, 18, 21, and 22 of the Dtsubgenome. The F1 and BC1F1 offspring from interspecific crosses differed in their transmission of univalents. Despite the regular pairing of chromosomes and the high meiotic index, interspecific aneuploid hybrids were characterized by a decrease in pollen fertility, which may indicate hidden structural variability in these genomes that did not affect meiotic division. The identification of chromosomes using chromosome-specific SSR markers in the early stages of plant development has greatly accelerated the detection of monosomic plants. The analysis of morphobiological traits revealed that monosomic F1 hybrids were more similar to the donor line, while BC1F1 hybrids were more similar to the recurrent parent but also showed previously undetected traits.

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“…For many years, the National University of Uzbekistan has been conducting studies on the induction of cotton plants with various chromosomal aberrations using various methods of induced mutagenesis. As a result, a unique cotton cytogenetic collection was created, including monosomic, monotelodisomic and translocation lines (Sanamyan et al, 2014). This article discusses the unified identification of univalent chromosomes in monosomic cotton lines using a well-defined tester set of cotton translocation lines with identified chromosomes.…”

Section: Introductionmentioning

confidence: 99%

Identification of univalent chromosomes in monosomic lines of the Gossypium hirsutum L. cotton type with the help of cytogenetic markers

2019

Plant Genetic Resources for Breeding and Producing Functional Nutraceutical Food

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Cytogenetic Collection of Uzbekistan

Cited by 8 publications

References 42 publications

Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Features of Chromosome Introgression from Gossypium barbadense L. into G. hirsutum L. during the Development of Alien Substitution Lines

Identification of univalent chromosomes in monosomic lines of the Gossypium hirsutum L. cotton type with the help of cytogenetic markers

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