Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides: micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells

Lucero, L; Pastor, Susana; Suárez, Sonia; Durban, Raquel; Gómez, C; Parrón, Tesifón; Creus, A.; Marcos, Ricard

doi:10.1016/s1383-5718(99)00200-4

Cited by 111 publications

(76 citation statements)

References 39 publications

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“…Thus, smoking had an additive effect on the frequency of BNMN and MNL, as more BNMN and MNL were found in the lymphocytes of smokers than non-smokers. On the other hand, micronuclei analyses conducted by Lucero et al (2000) and Pasastor et al (2002) had shown that smoking habits caused no significant elevation in chromosomal aberrations.All results of our study indicate that the level of cytogenetic damage was significantly affected by the subjects' exposure to pesticides. They also indicate a need for regular biomonitoring of persons occupationally exposed to various mixtures of pesticides.…”

Section: Discussionmentioning

confidence: 97%

Micronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticides

et al. 2015

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SUMMARYMicronucleus (MN) is a biomarker widely used in biomonitoring studies for determining the genetic risk associated with exposure to pesticides. The purpose of this study was to assess damage to the genetic material of workers occupationally exposed to pesticides as detected in micronucleus tests. The research included 119 subjects divided into three groups: a control group of 39 subjects, a group of 40 subjects exposed to pesticides as producers and a group of 40 pesticide applicators in the field. A Mann-Whitney U-test displayed statistically significant differences between the parameter means of all variables, and the control group. Significant differences were observed between males involved in pesticide production and application for the parameter MN4, then between non-smoking producers and applicators regarding parameters MN2, MN3, MN4 and NB, as well as between the control and applicator groups for parameter MN2, and between producers and applicators for parameter MN3. Spearman's correlation test showed a positive correlation between the frequency of micronuclei and age of respondents, as well as their smoking habits. A statistically significant difference in relation to cytogenetic parameters was detected between the respondents working in pesticide production and those working in the field. The results suggest that applicators in the field do not use adequate personal protective equipment. Regular biological monitoring of workers exposed to pesticides is required.

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Section: Discussionmentioning

confidence: 97%

Micronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticides

et al. 2015

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“…Such an association was not, however, unequivocally demonstrated in most of the few studies where SCEs or MN were used as biomarkers (48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58). Nevertheless, our MN results appear to support the increased sensitivity of the GSTM1 null genotype, because in smokers an occupational exposure effect could be seen only among GSTM1 null subjects.…”

Section: Discussionmentioning

confidence: 99%

Sister chromatid exchanges and micronuclei in peripheral lymphocytes of shoe factory workers exposed to solvents.

Pitarque

Vaglenov

Nosko

et al. 2002

Environ Health Perspect

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We examined sister chromatid exchanges (SCEs) and micronuclei (MN; cytokinesis-block method) in cultured peripheral lymphocytes from 52 female workers of two shoe factories and from 36 unexposed age- and sex-matched referents. The factory workers showed an elevated level of urinary hippuric acid, a biomarker of toluene exposure, and workplace air contained high concentrations of various organic solvents such as toluene, gasoline, acetone, and (in one of the plants only) ethylacetate and methylenediphenyl diisocyanate. The shoe factory workers showed a statistically significant higher frequency of micronucleated binucleate lymphocytes in comparison with the referents. This finding agreed with three preliminary MN determinations (each comprising 27-32 shoe workers and 16-20 controls) performed in one of the plants 2-5 years earlier. The shoe factory workers also had a lower average level of blood hemoglobin than the referents. In contrast, no difference was found between the groups in SCE analysis. Smokers showed significantly higher mean frequencies of SCEs per cell and high frequency cells (HFC) than nonsmokers. Aging was associated with increased MN rates and reduced cell proliferation. Polymorphism of the glutathione S-transferase M1 gene (GSTM1) did not affect the individual level of SCEs; but in smoking shoe workers an effect of the occupational exposure on the frequency of micronucleated cells could be seen only in GSTM1 null subjects. The low prevalence of the glutathione S-transferase T1 (GSTT1) null genotype precluded the evaluation of the influence of GSTT1 polymorphism. Our results show that the shoe factory workers have experienced genotoxic exposure, which is manifest as an increase in the frequency of MN, but not of SCEs, in peripheral lymphocytes. The exposures responsible for the MN induction could not be identified with certainty, but exposure to benzene in gasoline and methylenediphenyl diisocyanate may explain some of the findings.

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“…In not any case, correlation between the micronuclei frequency and the exposure time was observed or determined. Other authors have described negative results for the analysis of MN in both peripheral blood lymphocytes and exfoliated buccal cells realized at the same time (Lucero et al, 2000;Pastor et al, 2001aPastor et al, ,b, 2002bPastor et al, , 2003. Negative results were also found in peripheral blood lymphocytes, in oropharyngeal cells (Calvert et al, 1998) and in umbilical cord blood cells (Levario-Carrillo et al, 2005).…”

Section: Micronucleusmentioning

confidence: 91%

Pesticides: Genotoxic Risk of Occupational Exposure

Gómez‐Arroyo¹,

Martínez-Valenzuela²,

Villalobos‐Pietrini³

et al. 2011

Pesticides - The Impacts of Pesticides Exposure

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Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides: micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells

Cited by 111 publications

References 39 publications

Micronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticides

Micronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticides

Sister chromatid exchanges and micronuclei in peripheral lymphocytes of shoe factory workers exposed to solvents.

Pesticides: Genotoxic Risk of Occupational Exposure

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