Cytogenetic anomalies and causes for their occurrence in somatic cells

Kovaleva, Oxana

doi:10.1007/s11956-008-1008-2

Cited by 7 publications

(2 citation statements)

References 49 publications

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“…There is also an associative relationship between chromosomal instability and the reproductive function of animals (Starodub, 2012). Thus, partial autosome trisomy 1 and 15 leads to the death of a part of spermatozoa and a significant decrease in the fertilizing ability of sperm, chimerism of sex chromosomes in fertile causes a decrease in the quality of sperm production, and trisomy of sex chromosomes in females -a violation of desire and a defect of the skeleton (Kovaleva, 2008). Scientists are now increasingly interested in the study of karyotype variability of crossborder genetic resources of animals.…”

Section: Lyubov Starodub* and Nataliia Mokhnachovamentioning

confidence: 99%

Characteristics of the karyotype of Ukrainian buffalos

Starodub,

Mokhnachova

2023

Buffalo Bull.

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The article presents the results of studying the karyotype of the Ukrainian buffalo population. With the help of routine and GTG methods of analysis of metaphase plates of chromosomes, it was established that in the studied animals the diploid chromosome set was equal to fifty chromosomes (2n=50), which consisted of 5 pairs of meta- and submetacentric and 19 pairs of acrocentric autosomes and one pair (XX) or (XY) sex chromosomes. The total number of chromosome arms (FN) was 60, which correspond to animals of the water buffalo species (Bubalus bubalis), a subspecies of the river buffalo (B. b. Bubalis). Cytogenetic control revealed wide intraspecies limits of spontaneous somatic mutagenesis: absence of constitutive chromosomal disorders; the frequency of metaphases with aneuploidy, the average value (M±m) of which was equal to 10.5±0.13%, polyploidy (M±m=0.7±0.25%), asynchronous separation of the centromeric regions of chromosomes - (M±m=5.3±2.00), chromosomal breaks (M±m=0.7±0.24%), the proportion of cells with micronuclei (M±m=2.5±0.39‰), binucleated cells (M±m=2.6±0.32‰) and mitotic index (M±m=4.8±0.65‰). It has been established that Ukrainian river buffaloes are characterized by high karyotype stability.

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Section: Lyubov Starodub* and Nataliia Mokhnachovamentioning

confidence: 99%

Characteristics of the karyotype of Ukrainian buffalos

Starodub,

Mokhnachova

2023

Buffalo Bull.

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“…Кількість поліплоїдних клітин у коней двох господарств знаходилася у межі 1,1%-2,0%, середнє значення якого становило -1,4% і не перевищувало видові показники цієї мінливості [4]. Встановлено, що асинхронне розходження центромірних районів хромосом виникає у результаті передчасної реплікації прицентромірних гетерохроматинових ділянок, асоційованих з активністю центромери [6]. У гуцульських коней, яких досліджували, розмах цієї мінливості становив 2,0%-6,0%, при середньому значенні -3,9%, що відповідає спонтанному рівню для коней в цілому (2,2%-9,1%) [7].…”

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Cytogenetic Variability of the Hutsul Breed of Horses

Starodub

2023

Anim. Breed. and Genet.

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Goal. Study of features of karyotypic variability of Hutsul horses. Methods. Cytogenetic analysis, micronucleus test. Results. The average rate of genomic disorders (aneuploidy) was 10.8%, with a range of values of this variability of 8.8%–11.1% in animals of the LLC "Krai Neba" and the National Nature Park "Hutsulshchyna" with a statistically insignificant difference between the mean values. The multiple increase in the number of chromosomes, polyploidy, was in the range of 1.1%–2.0%, which does not exceed the species of this variability. The magnitude of the asynchronous divergence of the centromeric regions of the chromosomes was 2.0%–6.0%, which corresponds to the spontaneous level for horses as a whole (2.2%–9.1%). Structural chromosome abnormalities (chromosomal breaks) in the horses of the Krai Neba LLC farm were not detected, and in the animals of the Hutsulshchyna National Nature Park they were 1.1%. The range of variability of lymphocytes with a micronucleus for animals of two farms was 4.0–4.2‰. The number of dinuclear lymphocytes (6.2‰) exceeded the mitotic index (4.3‰) with an insignificant difference in the average values, which indicates the absence of radionuclide contamination of the territory. Conclusions. The average number of aneuploid cells – 10.8%, polyploid – 1.4%, asynchronous divergence of the centromeric regions of chromosomes – 3.9% and the proportion of chromosomal breaks – 1.1%. The proportion of lymphocytes with a micronucleus – 4.0–4.2–4, dinuclear lymphocytes – 6.2‰ and the mitotic index – 4.3‰, which indicates the stability of the karyotype of the studied horses.

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Micronucleus assay in bovine lymphocytes after exposure to bisphenol A in vitro

Šutiaková

Kovalkovičová

Šutiak

2014

In Vitro Cell.Dev.Biol.-Animal

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Bisphenol A (BPA) [2,2-bis-(4-hydroxyphenyl) propane] is an important industrial agent, made by combining acetone and phenol, that is used extensively as a monomer in the production of polycarbonate plastics and as a precursor of epoxy resins. Micronucleus assays have served as an index of cytogenetic damage in in vivo and in vitro studies. We studied the genotoxic and cytotoxic effects of BPA on bovine peripheral lymphocytes in vitro. Lymphocyte cultures from two donors were exposed to four different concentrations of BPA (1 × 10(-4), 1 × 10(-5), 1 × 10(-6), and 1 × 10(-7) mol.L(-1)) for 48 h. The highest concentration of BPA (1 × 10(-4) mol.L(-1)) resulted in a significant increase in the number of micronuclei in comparison with the negative control (67.50 ± 2.121/1,000 binucleated cells versus 36.0 ± 5.657/1,000 binucleated cells in the DMSO control, P = 0.018). BPA did not affect the nuclear division index at any treatment concentrations. The present results thus demonstrate a significant genotoxic effect by BPA on bovine peripheral lymphocytes in vitro, only at the highest concentration.

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Cytogenetic anomalies and causes for their occurrence in somatic cells

Cited by 7 publications

References 49 publications

Characteristics of the karyotype of Ukrainian buffalos

Characteristics of the karyotype of Ukrainian buffalos

Cytogenetic Variability of the Hutsul Breed of Horses

Micronucleus assay in bovine lymphocytes after exposure to bisphenol A in vitro

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