Cytogenetic and biological characterization of two new human plasma cell lines

Brox, L.; Belch, Andrew R.; Pollock, Edward J.; He, Xu; Braekeleer, Marc De; Lin, Chyi-Chyang

doi:10.1016/0165-4608(87)90268-8

Cited by 8 publications

(3 citation statements)

References 16 publications

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“…, 1985;Takahashi et al, 1985;Gazdar et a l . , 1986;Brox et al, 1987;Donelli et al, 1987;Jernberg et al, 1987). It is probable that in this minority of patients, additional oncogenic events allow the tumor clone to metastasize outside the bone-marrow environment and to generate permanently growing cell lines in vitro.…”

Section: Discussionmentioning

confidence: 99%

Limiting dilution cloning of B cells from patients with multiple myeloma: Emergence of non‐malignant B‐cell lines

Clofent

Klein

Commes

et al. 1989

Intl Journal of Cancer

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Multiple myeloma (MM) is a B-cell malignancy characterized by the accumulation of slowly proliferating malignant plasma cells in the bone marrow (BM). Several reports have shown the existence of an abnormal B-cell compartment including proliferative idiotypic B cells (i.e., B cells bearing the same idiotypic determinants as the myeloma protein) in the BM and peripheral blood (PB) of patients with MM. In order to study whether this abnormal compartment can be grown in vitro, we cultured the PB and BM of 23 patients with MM using limiting dilution methods. Our purpose was to restrict the effect of suppressor cells and the possible overgrowth of the cultures by the more rapidly growing B cells, which occurs in bulk cultures. Spontaneously growing cells were obtained only from patients seropositive for the Epstein-Barr virus (EBV) and all the cultures were composed of B cells carrying the EBV genome. Thus, positive cultures were generated only in the presence of B cells latently infected with EBV in vivo. The mean frequency of these B cells (1 in 25,000 B cells) was as low in MM patients as in healthy donors. This low frequency indicated that malignant cells do not bear the EBV genome in vivo and that the in vivo regulation of the EBV infection is unaffected in patients with MM. No Ig-gene rearrangements, specific of the autologous myeloma cells, were found in the cell lines obtained from BM or PB. Thus, the putative malignant B cells or myeloma cells were not able to generate cell lines in vitro, either spontaneously or after endogenous infection with EBV.

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Section: Discussionmentioning

confidence: 99%

Limiting dilution cloning of B cells from patients with multiple myeloma: Emergence of non‐malignant B‐cell lines

Clofent

Klein

Commes

et al. 1989

Intl Journal of Cancer

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“…-31 -Οι κυτταρογενετικές μελέτες έχουν δείξει μια μεγάλη διακύμανση των ανώμαλων καρυοτύπων σε ποσοστό που κειμαίνεται από 24%-76% των περιπτώσεων. 1973, Wetter 1980, Ueschima 1983, Sumegi 1985, Brox 1987, Wiernik 1987, Vip 1990, Smadja 1991, Bastard 1991, Mitelman F.1991.…”

Section: ζ ορολογιαunclassified

“…Ανακατατάξεις στο χρωμόσωμα 10 σε περιπτώσεις μυελώματος και πλασματοκυτταρικής λευχαιμίας έχουν ανακοινωθεί από τους Liang et al 1979, Ueshima et al, 1983, Petkovic et al, 1989, Brox et aL 1987, Bastard et al, 1989 (Simpson et al, 1987, Mathew et al, 1987.…”

Section: Elk-2unclassified