Multiple Myeloma Cell Lines

Jernberg-Wiklund, Helena; Nilsson, Kenneth

doi:10.1007/0-306-46877-8_4

Cited by 16 publications

(17 citation statements)

References 147 publications

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“…44 However, two of the cell lines, ARH-77 and IM-9, tested in that study are not true MM cell lines, but are EBV-positive lymphoblastoid cell lines. 31 Furthermore, another cell line tested by Fedyk et al, 44 U-266BL, is probably identical to U-266 1984 used in this study which was found to be unresponsive. Of the six MM cell lines tested in our study, U-266 1984 was the only cell line found to be unresponsive to SDF-1␣, or to any other chemokine tested.…”

Section: Figurementioning

confidence: 50%

“…30 All the cell lines are negative for EBV, express CD138, and are classified as true MM cell lines. 31 The cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/ml penicillin and 50 g/ml streptomycin (Life Technologies, Renfrewshire, UK). U-266 1970, U-1958, and HL407E are IL-6-dependent cell lines and were grown on a layer of IL-6-producing human fibroblast lines AG1523 (The Human Mutant Genetic Cell Repository, Camden, NJ, USA).…”

Section: Cellsmentioning

confidence: 99%

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Expression and function of chemokine receptors in human multiple myeloma

et al. 2003

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Section: Figurementioning

confidence: 50%

Section: Cellsmentioning

confidence: 99%

Expression and function of chemokine receptors in human multiple myeloma

et al. 2003

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“…The expression of the bcl‐2 family of genes has in some cases been demonstrated to be regulated by IL‐6 in MM cell lines (15, 30, 35, 36, 45). However, it is difficult to provide a coherent picture of the capacity of IL‐6 to regulate the expression of bcl‐2‐related genes, since there are few reports using MM cell lines exclusively dependent on exogenous IL‐6 for growth and/or survival (36, 42, 45, 47). To study the underlying mechanisms of the survival and/or growth effects mediated by IL‐6 deprivation in MM cells, we also took advantage of the possibility of dissociating the effects of IL‐6 in two IL‐6‐dependent cell lines.…”

Section: Discussionmentioning

confidence: 99%

Expression of the bcl‐2 family of pro‐ and anti‐apoptotic genes in multiple myeloma and normal plasma cells

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Strömberg

Georgii-Hemming

et al. 2002

European J of Haematology

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Aberrant expression of genes regulating apoptosis/survival seems to be essential in the stepwise development of human multiple myeloma (MM). In this paper we have compared the expression of bcl-2 family pro- and anti-apoptotic genes in MM cell lines, primary MM cells and normal plasma cells. The Bcl-2, Mcl-1, Bcl-xL/S, Bcl-w, Bax, Bak, and Bad were shown to be expressed in both malignant and non-neoplastic, normal plasma cells. Quantitative analysis revealed that the malignant phenotype seemed to correlate with an elevated expression of Mcl-1, a decreased expression of Bax and, to a lesser extent, an increased Bcl-2/Bax expression ratio. The possible influence of interleukin-6 (IL-6) in regulating the expression of the bcl-2-related genes was also examined. Using the IL-6-dependent MM cell lines U-1958 and U-266-1970 it was clearly shown that IL-6 deprivation induced cell cycle arrest in both cell lines, whereas apoptosis was only detected in the U-1958 cells. Furthermore, the anti-apoptotic proteins Bcl-2, Mcl-1 and Bcl-xL were down-regulated, while the expression of the pro-apoptotic Bax protein was increased. To conclude, we suggest that the expression pattern of the Bcl-2 family of proteins separates the malignant phenotype of MM from normal plasma cells, and that the protecting effect of IL-6 may be conducted via an altered balance between these proteins.

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“…Human multiple myeloma cell lines U266-1984(Nilsson et al, 1970), U-1958(Jernberg-Wiklund and Nilsson, 2000, Karpas 707 (Karpas et al, 1982); the human monoblastic cell line U-937 (Sundstrom and Nilsson, 1976); the T-cell line Jurkat (Santos-Rosa et al, 2002); and the Burkitt's lymphoma BJAB (Menezes et al, 1975) were maintained in RPMI 1640 (SigmaAldrich, St. Louis, USA) medium plus 10% FBS and antibiotics in a humidified atmosphere containing 5% CO 2 at 37°C. PBMC (peripheral blood mononuclear cells) were prepared by Ficoll-Hypaque separation of buffy coats from healthy blood donors.…”

Section: Cell Linesmentioning

confidence: 99%