Cytogenetic analysis of the Brazilian blind catfish<i>Pimelodella kronei</i>and its presumed ancestor,<i>P. transitoria</i>

Almeida-Toledo, Lurdes Foresti de; Foresti, Fausto; Trajano, Eleonora; Filho, Sílvio de Almeida Toledo

doi:10.1080/00087114.1992.10797229

Cited by 30 publications

(23 citation statements)

References 11 publications

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“…Among Heptapteridae fish, the diploid number ranges from 2n = 46 chromosomes in Pimelodella avanhandavae (Vissoto et al, 1999) and Pimelodella aff. meeki (Dias and Giuliano-Caetano, 2002), to 58 chromosomes in Cetopsorhamdia iheringi (Vissoto et al, 1999), Pimelodella kronei (Almeida-Toledo et al, 1992), and Rhamdia species (R. hillari, Fenocchio and Bertollo, 1990;R. branneri and R. voulezi, Abucarma and Martins-Santos, 2001; R. quelen, Stivari and Martins-Santos, 2004).…”

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confidence: 99%

Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)

Margarido

Moreira‐Filho

2008

Genet. Mol. Biol.

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The Neotropical Heptapteridae fish Imparfinis hollandi, endemic to the Iguaçu River Basin (Brazil), was cytogenetically analyzed and the diploid chromosome number of 2n = 42 chromosomes was determined (22m + 10sm + 10st), the lowest diploid number in this genus and family. Like other Heptapteridae species, only one NOR-bearing chromosome pair was detected by silver nitrate staining. Dark heterochromatic blocks were visualized in only three chromosome pairs, and chromomycin A 3 + bands were coincident with Ag-NORs. Although no intercalary (TTAGGG) n sequence was observed through FISH with a telomere probe, an asymmetric karyotype showing four large chromosome pairs with diploid chromosome number reduction suggests that tandem chromosome fusions probably occurred during the karyotypic differentiation of Imparfinis hollandi.

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confidence: 99%

Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)

Margarido

Moreira‐Filho

2008

Genet. Mol. Biol.

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“…Similarities between cytogenetic data of epigean and troglobitic fish forms have already been described by Almeida-Toledo et al (1992) that carried out cytogenetic studies in specimens of Pimelodella kronei and its putative sister species Pimelodella transitoria collected in three caves in the State of São Paulo. Both species presented the same karyotype formulae and shared identical patterns of C bands and number and location of NORs, however, a supernumerary microchromosome in one individuals of Pimelodella kronei was identified.…”

Section: Discussionmentioning

confidence: 94%

Cytogenetic and molecular analyses in troglobitic and epigean species of Pimelodella (Siluriformes: Heptapteridae) from Brazil

et al. 2012

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Samples from seven different locations of the genus Pimelodella were genetically examined, two caves (exclusively subterranean, upper Tocantins River and São Francisco River) and five epigean (from upper Paraná River basin). Cytogenetic analyses revealed the same diploid number (2n=46) for all species besides similarities in both number and location of nucleolar organizer regions and C bands. FISH with 5S rDNA probes and CMA 3 staining indicated significant differences among the studied species. Application of PCR-RFLP in ATPase 6 and 8 mitochondrial genes allowed building a minimum evolution phenogram identifying the close evolutionary relationship among groups. Both chromosomal and molecular data were useful to infer the relationships among studied Pimelodella species.Amostras de sete diferentes localidades do gênero Pimelodella foram geneticamente analisadas, duas cavernícolas (exclusivamente subterrâneas, alto rio Tocantins e rio São Francisco) e cinco epígeas (provenientes da bacia do alto Paraná). Análises citogenéticas revelaram o mesmo número diploide (2n=46) para todas as espécies, além de similaridades no número e localização das regiões organizadoras de nucléolo e bandas C. FISH com sondas de rDNA 5S e marcação com CMA 3 indicaram diferenças significativas entre as espécies estudadas. A aplicação da técnica de PCR-RFLP nos genes mitocondriais ATPase 6 e 8 permitiu a construção de um fenograma de evolução mínima identificando uma estreita relação evolutiva entre as espécies estudadas.

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“…avanhandavae (Swarça et al 2003a), 2nϭ56 in Imparfinis cf. piperatus (Vissotto et al 2001), Rhamdella microcephala (Fonseca et al 2003), and 2nϭ58 in Rhamdia quelen (Hochberg andErdtmann 1988, Fenocchio andBertollo 1990), Pimelodella kronei and Pimelodella transitoria (Almeida-Toledo et al 1992), Imparfinis mirini (Vissotto et al 1997), Cetopsorhamdia iheringi (Vissotto et al 1999a) and Imparfinis piperatus (Vissotto et al 2001). Among Pimelodidae, 2nϭ50 chromosomes were found in Calophysus macropterus (Ramirez-Gil et al 1998), Pirinampus pirinampu (Swarça et al 1999), and 2nϭ56 in Pseudoplatystoma fasciatum and P. tigrinum (Fenocchio and Bertollo 1992), Sorubim lima (Fenocchio and Bertollo 1992), in Bergiaria westermanni (Dias and Foresti, 1993), Pimelodus maculatus (Dias and Foresti 1993), P. argenteus and P. mysteriosus (Souza et al 2003a Pseudoplatystoma corruscans (Martins-Santos et al 1996), Hemisorubim platyrhynchos (MartinsSantos et al 1996), Zungaro zungaro (Martins-Santos et al 1996), Iheringichthys labrosus (Vissotto et al 1999b), and Steindachneridion sp.…”

Section: Resultsmentioning

confidence: 99%

Cytogenetic Analyses of Pseudopimelodus mangurus (Teleostei: Siluriformes: Pseudopimelodidae)

2004

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SummaryThe cytogenetic analyses showed that Pseudopimelodus mangurus has a diploid number of 2nϭ54 chromosomes (6M, 26SM, 12ST and 10A), single Ag-NORs on the short arm of a middle-sized ST pair, identified as pair 19, and a very small amount of C-band positive segments in two chromosome pairs. The Ag-NORs are C-band positive. The staining of the chromosomes of P. mangurus with CMA 3 reveled the occurrence of bright signals corresponding to the Ag-NORs segments, to the C-band positive segments and also to some C-band negative segments. The occurrence of a diploid number of 2nϭ54 in all species of the family Pseudopimelodidae and its absence among representatives of Pimelodidae and Heptapteridae, two related families previously considered, reinforces the hypothesis that Pseudopimelodidae is a monophyletic group. Key words Karyotype, chromosomes, Ag-NORs, C-band, Chromomycin A 3The order Siluriformes (catfishes) has 2,405 species, divided into 34 families and 412 genera, worldwide distributed, except for the coldest areas in the Southern and Northern Hemispheres (Nelson 1994). Recent phylogenetic studies showed that the old family Pimelodidae comprised three monophyletic units: Pimelodidae, Heptapteridae and Pseudopimelodidae (Lundberg et al. 1991, de Pinna 1998, Britto 2003. Thus, Pseudopimelodidae with only 26 described species widely distributed in South America, can be considered the least known family among the naked Neotropical freshwater catfishes (Shibatta 2003). According to Shibatta (2003), Pseudopimelodidae is composed by the genera Batrochoglanis (4 species), Cephalosilurus (4 species), Lophiosilurus (1 species), Microglanis (12 species) and Pseudopimelodus (5 species).Only the karyotypes of Microglanis cottoides (Vissotto et al. 1999a), Lophiosilurus alexandri (Marques et al. 2002) and Pseudopimelodus bufonius (Souza et al. 2003b) were so far described. The present study had as main objective characterizing the karyotype of P. mangurus and the data obtained were compared with those available from other species of Pseudopimelodidae, Pimelodidae and Heptapteridae. Material and methodsSeven specimens of Pseudopimelodus mangurus (6 males, 1 female) from Mogi-Guaçu river (Pirassununga, São Paulo, Brazil) were karyotyped. Fishes were identified and deposited in the fish collection of the Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, São Paulo, Brazil.Mitotic chromosome preparations were performed according to the technique described by Foresti et al. (1993). The silver-staining of nucleolar organizer regions (Ag-NORs) followed the technique proposed by Howell and Black (1980), C-banding was performed by the method of Sum-

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Cytogenetic analysis of the Brazilian blind catfishPimelodella kroneiand its presumed ancestor,P. transitoria

Cited by 30 publications

References 11 publications

Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)

Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)

Cytogenetic and molecular analyses in troglobitic and epigean species of Pimelodella (Siluriformes: Heptapteridae) from Brazil

Cytogenetic Analyses of Pseudopimelodus mangurus (Teleostei: Siluriformes: Pseudopimelodidae)

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