Cytogenetic Analysis of Esophageal Squamous Cell Carcinoma Cell Lines by Comparative Genomic Hybridization

Tada, Kohsuke; Oka, M.; Hayashi, Hiroto; Tangoku, Akira; Oga, Atsunori; Sasaki, Kohsuke

doi:10.1016/s0165-4608(99)00160-0

Cited by 28 publications

(20 citation statements)

References 10 publications

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“…Earlier reports have identified multiple abnormal regions in ESCC, including amplifications at 1p34, 3q, 5p, 7p12, 8q, 11q13, 12p, 17q12, and 22q as well as deletions at 2q, 3p, 4q, 5q13-q21, 9p21.3, and 13q (5,7,8,(14)(15)(16)(17)(18)(19)(20)(21). Our study further narrowed these altered chromosome regions and identified candidate amplification-or homozygous deletion-associated genes.…”

Section: Discussionsupporting

confidence: 67%

“…However, there were some differences. For example, gains of chromosomes 16,17,19, and 22 were more common than their losses in our study, whereas the frequencies of gain and loss in chromosomes 16 and 17 were similar, and the loss of chromosomes 19 and 22 was more dominant in the Progenetix database. In our study, gain of 9q34.11-34.3 was detected in 53% of ESCCs, whereas the frequency of 9q gain is reported as less than 20% in the Progenetix database.…”

Section: Discussioncontrasting

confidence: 49%

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Consistent and Differential Genetic Aberrations between Esophageal Dysplasia and Squamous Cell Carcinoma Detected By Array Comparative Genomic Hybridization

Shi

Jiang

et al. 2013

Clinical Cancer Research

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Purpose: Our aim was to identify frequent genomic aberrations in both esophageal squamous cell carcinoma (ESCC) and esophageal dysplasia and to discover important copy number-driving genes and microRNAs (miRNA) in ESCC.Experimental Design: We conducted array-based comparative genomic hybridization (array CGH) on 59 ESCC resection samples and 16 dysplasia biopsy samples. Expression of genes at 11q13.3 was analyzed by real-time PCR (RT-PCR) and immunohistochemistry (IHC). Integrated analysis was conducted to identify genes or miRNAs with copy number-expression correlations.Results: Array CGH identified 11 amplifications and eight homozygous deletions in ESCC. Integrated analysis of array CGH data with matched gene expression microarray data showed that 90 overexpressed genes and 24 underexpressed genes were consistent with DNA copy number changes, including 12 copy number-driving miRNAs. In esophageal dysplasia, six gains, four losses, 12 amplifications, and four homozygous deletions were detected. Amplifications of 7p11.2 and 11q13.2-11q13.3 (CCND1) and homozygous deletion at 9p21.3 (CDKN2A) were consistent genomic changes in both dysplasia and carcinoma. ANO1 at 11q13.3 was overexpressed at the mRNA and protein levels in tumors, and higher mRNA expression was correlated with the copy number increase. In particular, ANO1 expression was elevated in moderate dysplasia compared with normal esophageal epithelium. IHC revealed that ANO1 overexpression was positively correlated with lymph node metastasis and advanced clinical stage. Knockdown of ANO1 significantly inhibited the proliferation of KYSE30 and KYSE510 cells.Conclusion: Copy number aberrations in both esophageal dysplasia and ESCC may be useful as potential biomarkers for early detection. In addition, ANO1 may be a candidate target gene in esophageal tumorigenesis.

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Section: Discussionsupporting

confidence: 67%

Section: Discussioncontrasting

confidence: 49%

Consistent and Differential Genetic Aberrations between Esophageal Dysplasia and Squamous Cell Carcinoma Detected By Array Comparative Genomic Hybridization

Shi

Jiang

et al. 2013

Clinical Cancer Research

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“…Copy number increases of the 3q26-qter region have been identified in different SCCs, including lung, oesophagus and cervix. [27][28][29][30] The presence of frequent copy number gains/amplifications suggested the presence of a relevant proto-oncogene in this region.…”

Section: Discussionmentioning

confidence: 99%

High sex determining region Y-box 2 expression is a negative predictor of occult lymph node metastasis in early squamous cell carcinomas of the oral cavity

Züllig

Roessle

Weber

et al. 2013

European Journal of Cancer

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“…Previous studies show frequent allele losses on chromosomes 9 and 18 by both loss of heterozygosity (LOH) (Aoki et al, 1994;Mori et al, 1994;Shibagaki et al, 1994;Wang et al, 1996;Muzeau et al, 1997;Naidoo et al, 1999;Karkera et al, 2000;Yang et al, 2004) and comparative genomic hybridization (CGH) studies (Pack et al, 1999;Tada et al, 2000;Yen et al, 2001Yen et al, , 2003. These results suggest that tumor suppressor genes (TSGs) associated with esophageal tumorigenesis may be located on these chromosomes.…”

Section: Introductionmentioning

confidence: 94%

Tumor suppressive role of a 2.4 Mb 9q33–q34 critical region and DEC1 in esophageal squamous cell carcinoma

Yang

Leung

et al. 2004

Oncogene

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The key genes involved in the development of esophageal squamous cell carcinoma (ESCC) remain to be elucidated. Previous studies indicate extensive genomic alterations occur on chromosome 9 in ESCC. Using a monochromosome transfer approach, this study provides functional evidence and narrows down the critical region (CR) responsible for chromosome 9 tumor suppressing activity to a 2.4 Mb region mapping to 9q33-q34 between markers D9S1798 and D9S61. Interestingly, a high prevalence of allelic loss in this CR is also observed in primary ESCC tumors by microsatellite typing. Allelic loss is found in 30/ 34 (88%) tumors and the loss of heterozygosity (LOH) frequency ranges from 67 to 86%. Absent to low expression of a 9q32 candidate tumor suppressor gene (TSG), DEC1 (deleted in esophageal cancer 1), is detected in four Asian ESCC cell lines. Stably expressing DEC1 transfectants provide functional evidence for inhibition of tumor growth in nude mice and DEC1 expression is decreased in tumor segregants arising after long-term selection in vivo. There is 74% LOH in the DEC1 region of ESCC primary tumors. This study provides the first functional evidence for the presence of critical tumor suppressive regions on 9q33-q34. DEC1 is a candidate TSG that may be involved in ESCC development.

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Cytogenetic Analysis of Esophageal Squamous Cell Carcinoma Cell Lines by Comparative Genomic Hybridization

Cited by 28 publications

References 10 publications

Consistent and Differential Genetic Aberrations between Esophageal Dysplasia and Squamous Cell Carcinoma Detected By Array Comparative Genomic Hybridization

Consistent and Differential Genetic Aberrations between Esophageal Dysplasia and Squamous Cell Carcinoma Detected By Array Comparative Genomic Hybridization

High sex determining region Y-box 2 expression is a negative predictor of occult lymph node metastasis in early squamous cell carcinomas of the oral cavity

Tumor suppressive role of a 2.4 Mb 9q33–q34 critical region and DEC1 in esophageal squamous cell carcinoma

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