Cytogenetic analysis of Epinephelus marginatus (Pisces: Serranidae), with the chromosome localization of the 18S and 5S rRNA genes and of the (TTAGGG) n telomeric sequence

Sola, Luciana; Innocentiis, Sabina De; Gornung, Ekaterina; Papalia, Silvia; Rossi, Anna Rita; Marino, G.; Marco, Patrizia De; Cataudella, Stefano

doi:10.1007/s002270000334

Cited by 39 publications

(56 citation statements)

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“…A similar lack of response of the pericentromeric heterochromatic domain after base-specific fluorochrome staining as that observed in the two populations studied by us has also been found in the chromosomes of other fish species (Sola et al, 1992;Rossi et al, 1996;Sola et al, 2000) and of other A. scabripinnis populations (Souza and Moreira-Filho, 1995;Souza et al, 1996). Considering the common pattern displayed by the A. scabripinnis populations studied to date, it may be that pericentromeric heterochromatins contain sequences with preserved compositions (seen in several species of the family Sparidae, see Garrido-Ramos et al, 1995) and preserved structures, since pericentromeric heterochromatin plays a fundamental role in the centromeric structure (Haaf et al, 1992).…”

Section: Discussionsupporting

confidence: 86%

Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)

et al. 2004

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The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC-and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive heterochromatins. Such differences in chromosome banding patterns together with other karyotypic differences suggest differentiation of these populations at taxonomic level.

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Section: Discussionsupporting

confidence: 86%

Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)

et al. 2004

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“…Data from the literature indicate that this assumption may be true only for some species. In fact, among vertebrates, an adjacent disposition between NORs and telomeres is reported to be an unusual finding (see Liu and Fredga, 1999) and a co-localization of 18S-28S and 5S rDNAs is not considered the rule, but rather the exception (Sola et al, 2000;Mandrioli et al, 2000). For example, in fish, a co-localization of repeated units of ribosomal DNAs has been described only in four out of 20 species so far analyzed (Pendás et al, 1994;Morán et al, 1996;Mandrioli et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

FISH mapping of 18S-28S and 5S ribosomal DNA, (GATA)n and (TTAGGG)n telomeric repeats in the periwinkle Melarhaphe neritoides (Prosobranchia, Gastropoda, Caenogastropoda)

et al. 2002

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“…Our results showed a single 5S rDNA site located in a different chromosome than the 18S rDNA sites. The presence of only one 5S rDNA site is common among eukaryotes, and the distinct chromosomal locations of 5S and 18S rDNA sites has been frequently reported for vertebrates (Mandrioli et al, 2000;Sola et al, 2000;Martins and Galetti, 2001). In protostome invertebrates, this kind of arrangement was described in some mollusks (López-Piñ ón et al, 2005;Insua et al, 2006;Huang et al, 2007).…”

Section: Mapping Of Repetitive Dnas In D Geminatus Dc Cabral-de-mellmentioning

confidence: 94%

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

2009

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Chromosomal banding techniques and repetitive DNA mapping are useful tools in comparative analysis and in the elucidation of genome organization of several groups of eukaryotes. In this study, we contributed to the knowledge of Coleoptera genomes by reporting the chromosomal organization of repetitive DNA sequences, as well as the presence and characteristics of a B chromosome in two natural populations of Dichotomius geminatus (Coleoptera; Scarabaeidae) using classical, chromosomal banding and molecular cytogenetic techniques. As in other coleopteran species, the heterochromatin was mainly concentrated in pericentromeric regions and the B chromosome was composed almost entirely of heterochromatin. Physical mapping using double fluorescent in situ hybridization was performed for the first time in Coleoptera; using DNA probes for 5S and 18S ribosomal RNA (rRNA) and histone H3 genes, we showed that ribosomal 18S rDNAs are located in chromosomes 3 and 4, whereas 5S rRNA and histone H3 genes are colocalized in chromosomal pair 2 and show an apparently interspersed organization. Moreover, these genes are not present in the B chromosome, suggesting that the B chromosome did not originate from chromosomal pairs 2, 3 or 4. On the other hand, mapping of the C 0 t-1 DNA fraction showed that the B chromosome is enriched in repetitive DNA elements, also present in the standard complement, indicating an intraspecific origin of this element in D. geminatus. These results will contribute to our understanding of genome organization and evolution of repetitive elements in Coleoptera and other insects regarding both A and B chromosomes.

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Cytogenetic analysis of Epinephelus marginatus (Pisces: Serranidae), with the chromosome localization of the 18S and 5S rRNA genes and of the (TTAGGG) n telomeric sequence

Cited by 39 publications

References 0 publications

Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)

Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)

FISH mapping of 18S-28S and 5S ribosomal DNA, (GATA)n and (TTAGGG)n telomeric repeats in the periwinkle Melarhaphe neritoides (Prosobranchia, Gastropoda, Caenogastropoda)

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

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