FISH mapping of 18S-28S and 5S ribosomal DNA, (GATA)n and (TTAGGG)n telomeric repeats in the periwinkle Melarhaphe neritoides (Prosobranchia, Gastropoda, Caenogastropoda)

Colomba, Mariastella; Vitturi, R.; Castriota, Luca; Bertoni, R; Libertini, Angelo

doi:10.1038/sj.hdy.6800070

Cited by 28 publications

(17 citation statements)

References 22 publications

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“…In addition, the same technique with biotinlabelled (TTAGGG) n and digoxigenin-labelled 18S-28S rDNA probes revealed that ribosomal and telomeric signals were adjacent. A similar configuration has been observed in a periwinkle (Colomba et al, 2002) and a slug (Vitturi et al, 2004). Among vertebrates, an adjacent disposition between telomere and NORs has been reported to be an unusual finding (Liu & Fredga, 1999).…”

Section: Discussionmentioning

confidence: 70%

“…Among vertebrates, an adjacent disposition between telomere and NORs has been reported to be an unusual finding (Liu & Fredga, 1999). However, this condition occurs more frequently in invertebrates than in vertebrates (Colomba et al, 2002). Nevertheless, the significance of the telomere within NORs is unclear.…”

Section: Discussionmentioning

confidence: 99%

“…The other repetitive sequence used frequently by FISH is (TTAGGG) n , which occurs in the telomere of all vertebrate chromosomes, whereas in invertebrates it is present in some species (Colomba et al, 2002) but not in others (Vitturi et al, 2000). In bivalve molluscs, this vertebrate telomeric sequence has been mapped using FISH in oysters (Guo & Allen, 1997;Wang & Guo, 2001), clams (Wang & Guo, 2001) and mussels (Plohl et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Mapping of ribosomal DNA and (TTAGGG)n telomeric sequence by FISH in the bivalve Patinopecten yessoensis (Jay, 1857)

Huang¹,

Hu²,

Hu³

et al. 2007

Journal of Molluscan Studies

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The chromosome set of Patinopecten yessoensis (Jay, 1857) was characterized using Giemsa staining, DAPI staining and fluorescence in situ hybridization (FISH) with three repetitive DNA probes [18S-28S rDNA, 5S rDNA and telomeric (TTAGGG) n ]. DAPI staining showed that AT-rich regions were located on the centromere of almost all chromosomes and interstitial banding was not observed. FISH showed that 18S-28S rDNA spread over the short arms of two subtelocentric chromosome pairs and 5S rDNA was located on the long arm of one subtelocentric chromosome pair. Sequential FISH demonstrated that 18S-28S and 5S rDNA were located on different chromosomes. FISH also showed that the vertebrate telomeric sequence (TTAGGG) n was located on both ends of each chromosome and no interstitial signals were detected. Sequential 18S-28S rDNA and (TTAGGG) n FISH indicated that repeated units of the two multicopy families were closely associated on the same chromosome pair.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mapping of ribosomal DNA and (TTAGGG)n telomeric sequence by FISH in the bivalve Patinopecten yessoensis (Jay, 1857)

Huang¹,

Hu²,

Hu³

et al. 2007

Journal of Molluscan Studies

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“…Although sinteny or even a close ligation of the18S-28S and 5S rDNA is not a rare phenomenon in animals (e.g. Drouin 1999;Colomba et al, 2002;Vitturi et al, 2002), the most frequent situation is that the two types of rDNA map on different chromosomes (e.g. Wimber & Steffensen, 1970;Matsuda et al, 1994;Kost et al, 1995;Martins & Galetti, 2001).…”

Section: Discussionmentioning

confidence: 98%

Karyotype and Chromosomal Location of 18S–28S and 5S Ribosomal DNA in the Scallops Pecten maximus and Mimachlamys varia (Bivalvia: Pectinidae)

et al. 2006

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This work describes the karyotype and chromosomal location of the ribosomal DNA (rDNA) of Pecten maximus and Mimachlamys varia, two commercial scallop species from Europe. According to the chromosome centromeric index values found, the karyotype of P. maximus is composed of 1 metacentric, 2 metacentric-submetacentric, 1 telocentric-subtelocentric and 15 telocentric pairs, and that of M. varia of 4 metacentric, 2 subtelocentric-submetacentric, 9 subtelocentric, 3 subtelocentric-telocentric and 1 telocentric-subtelocentric pairs. In P. maximus, 18S-28S rDNA was located by FISH on a metacentric-submetacentric pair, and in M. varia on a subtelocentric-submetacentric pair using both silver staining and FISH. PCR amplification of the 5S rDNA unit yielded a single product of about 460 bp (P. maximus) and 450 bp (M. varia), that used as probe revealed a 5S rDNA site on a telocentric pair in P. maximus and a subtelocentric pair in M. varia. Two-color FISH or sequential silver staining of 5S rDNA-FISH-metaphases corroborated that the two gene families are located on different chromosomes in both species. A comparative analysis of the data allowed the inference of karyotypic relationships within scallops.

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“…The 5S rRNA genes have been primarily mapped in fish [Martins and Galetti, 1999;Martins and Wasko, 2004;Pisano and Ghigliotti, 2009], and the histone genes have been mapped in a few species, for example in mammals, amphibians, fish, mollusks, and some insects [Graves et al, 1985;Tripputi et al, 1986;Turner et al, 1988;Hankeln el al., 1993;Pendás et al, 1994;Ranz et al, 2003;Zhang et al, 2007;Cabrero et al, 2009;Cabral-de-Mello et al, 2010a, 2011a. These multigene families can be organized in separated clusters or can be associated, as has been described for example in arthropods, annelids, mollusks, and fish [Andrews et al, 1987;Drouin et al, 1987Drouin et al, , 1992Barzotti et al, 2000;Vitturi et al, 2002;Colomba et al, 2002;Eirín-López et al, 2004;Vitturi et al, 2004;Pisano and Ghigliotti, 2009;Cabral-de-Mello et al, 2010a, 2011a.…”

mentioning

confidence: 99%

Cytogenetic Mapping of rRNAs and Histone H3 Genes in 14 Species of Dichotomius (Coleoptera, Scarabaeidae, Scarabaeinae) Beetles

Cabral-de-Mello

Moura

Martins

2011

Cytogenet Genome Res

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Standard cytogenetic analyses and chromosomal mapping of the genes for 18S and 5S rRNAs and histone H3 were performed in 14 species of beetles of the genus Dichotomius (Coleoptera, Scarabaeidae, Scarabaeinae). Conserved karyotypes with 2n = 18 and biarmed chromosomes were observed in all species. Moreover, the presence of a large metacentric pair (pair 1) was characteristic in the studied species, evidencing a remarkable synapomorphy for this genus, which probably originated by an ancient fusion of 2 autosomes while the ancestral sex-chromosome pair remained conserved. FISH showed that the 5S rRNA and histone H3 genes are located in the proximal region of pair 2, with the 2 genes co-located. However, the major rDNA cluster probed by the 18S rRNA gene mapped to 1–3 bivalents, being exclusively autosomal, associated with sex elements, or both. In most species, the major rDNA cluster was observed in pair 3, and it was frequently (64.3%) located in the distal region regardless of the chromosome. The conserved number and position of the 5S rDNA/H3 histone cluster seems to be an ancient pattern shared by all of the studied species. In contrast, the major rDNA clusters apparently tolerate distinct patterns of diversification in the karyotypes of the species that could be associated with small inversions, ectopic recombination, and transposition. Moreover, we reinforced the association/co-localization between the 5S rRNA and histone H3 genes in this group contributing thus to the knowledge about the chromosomal organization and diversification patterns of multigene families in beetles and insects.

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FISH mapping of 18S-28S and 5S ribosomal DNA, (GATA)n and (TTAGGG)n telomeric repeats in the periwinkle Melarhaphe neritoides (Prosobranchia, Gastropoda, Caenogastropoda)

Cited by 28 publications

References 22 publications

Mapping of ribosomal DNA and (TTAGGG)n telomeric sequence by FISH in the bivalve Patinopecten yessoensis (Jay, 1857)

Mapping of ribosomal DNA and (TTAGGG)n telomeric sequence by FISH in the bivalve Patinopecten yessoensis (Jay, 1857)

Karyotype and Chromosomal Location of 18S–28S and 5S Ribosomal DNA in the Scallops Pecten maximus and Mimachlamys varia (Bivalvia: Pectinidae)

Cytogenetic Mapping of rRNAs and Histone H3 Genes in 14 Species of Dichotomius (Coleoptera, Scarabaeidae, Scarabaeinae) Beetles

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