Cytocompatibility evaluation in cell-culture systems of chemically crosslinked chitosan/PVA hydrogels

Mansur, Herman S.; Junior, Ezequiel de Souza Costa; Mansur, Alexandra A.P.; Barbosa-Stancioli, Edel Figueiredo

doi:10.1016/j.msec.2008.12.012

Cited by 134 publications

(65 citation statements)

References 28 publications

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“…Good agreement was found with recent research reporting the FT-IR spectra for CS/PVA/GA byMansur et al (2009);Hu et al (2013) and CS/PVA/GEN byBispo et al (2010);Muzzarelli (2009).Although previous research has reported FT-IR analyses for CS/PVA, 260 CS/PVA/GA and CS/PVA/GEN crosslinked blends, their molecular structures have not been completely understood. Furthermore, we proposed the possible structures of the crosslinked hydrogels.Fig.…”

supporting

confidence: 88%

Influence of natural and synthetic crosslinking reagents on the structural and mechanical properties of chitosan-based hybrid hydrogels

Garnica-Palafox

Sánchez-Arévalo

2016

Carbohydrate Polymers

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supporting

confidence: 88%

Influence of natural and synthetic crosslinking reagents on the structural and mechanical properties of chitosan-based hybrid hydrogels

Garnica-Palafox

Sánchez-Arévalo

2016

Carbohydrate Polymers

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“…One method of obtaining the required properties from natural and synthetic polymers is to blend and crosslink them 20 .…”

Section: Introductionmentioning

confidence: 99%

Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

et al. 2016

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ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. AbstractThere is a need to develop pro-angiogenic biomaterials to promote wound healing and to assist in regenerative medicine. To this end, various growth factors have been exploited which have the potential to promote angiogenesis. However these are generally expensive and labile which limits their effectiveness. An alternative approach is to immobilize heparin onto biocompatible degradable hydrogels. The heparin in turn will then bind endogenous proangiogenic growth factors to induce formation of new blood vessels.In this study we continue our development of hydrogels for wound healing purposes by exploring covalently cross-linking chitosan (CS) and polyvinyl alcohol (PVA) hydrogels using triethyl orthoformate (TEOF) .Two concentrations of TEOF ( 4 and 16 %) were compared for their effects on the structure of hydrogels -their swelling, pore size and rate of degradation and 2 for their ability to support the growth of cells and for their heparin binding capacity and their effects on angiogenesis in a chick chorioantoic membrane assay .Hydrogels formed with 4 or 16% both TEOF cross-linker were equally cyto-compatible.Hydrogels formed with 4% TEOF absorbed slightly more water than those made with 16%TEOF and broke down slightly faster than non-cross-linked hydrogels. When soaked in heparin the hydrogel formed with 16% TEOF showed more blood vessel formation in the CAM assay than that formed with 4% TEOF.

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“…Four hydroxyl groups of the PVA are supposed to be connected by one GA molecule. The crosslinking reaction using GA is one of the most popular methods in biological systems due to its biocompatibility and biodegradability (Goissis et al, 1999;Mansur et al, 2009). Considering the amount of hydroxyl groups in the PVA, three different types of particle sensors are fabricated by changing the amount of GA. For the case of particle #1, 0.00625 mol of GA is incorporated, which corresponds to 2.5 times of the possible crosslinker units in PVA.…”

Section: Resultsmentioning

confidence: 99%

Flow tracing microparticle sensors designed for enhanced X-ray contrast

Lee

Jung

Ahn

2010

Biosensors and Bioelectronics

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Cytocompatibility evaluation in cell-culture systems of chemically crosslinked chitosan/PVA hydrogels

Cited by 134 publications

References 28 publications

Influence of natural and synthetic crosslinking reagents on the structural and mechanical properties of chitosan-based hybrid hydrogels

Influence of natural and synthetic crosslinking reagents on the structural and mechanical properties of chitosan-based hybrid hydrogels

Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

Flow tracing microparticle sensors designed for enhanced X-ray contrast

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