Cytocompatibility, biofilm assembly and corrosion behavior of Mg-HAP composites processed by extrusion

Abstract

In this work the cytocompatibility of pure magnesium and Mg-xHAP composites (x=5, 10 and 15wt%) fabricated by powder metallurgy routes has been investigated. The materials were produced from raw HAP powders with particle mean sizes of 6μm (S-xHAP) or 25μm (L-xHAP). The biocompatibility study has been performed for MC3T3 cells (osteoblasts/osteoclasts) and L929 fibroblasts. The results indicate that S-Mg (pure magnesium), S-10HAP and L-10HAP composites are the materials with the best biocompatibility. The ability of S. aureus bacteria to assemble biofilms was also evaluated. Biofilm formation assays showed that these materials are not particular prone to colonization and biofilm assembly is strain dependent. The corrosion resistance of S-Mg, S-10HAP and L-10HAP materials immersed in the media used for the cells culture has also been analyzed. Different trends in the corrosion resistance have been found: S-Mg and S-10HAP show a very high resistance to corrosion whereas the corrosion of L-10HAP steadily increases with time.

Bone tissue engineering potentials of 3D printed magnesium‐hydroxyapatite in polylactic acid composite scaffolds

“…Also, the increased activity of Mg‐HAp/PVA composite towards both of the bacterial cells as against the pure HAp can be linked to the presence of Mg and was supported by the literature studies. For example, Del Campo et al 33 indicated in their report of Mg/HAp composite that the varied size and Mg/HAp ratios have led to the formation of two methicillin‐resistant S aureus strains and concluded that the biofilm development relies on the type of strain and Mg concentration. In another study, 34 the antimicrobial efficiency of Mg‐HAp against P aeruginosa , S aureus , and Candida albicans microbial bacterial strains was found to be very efficient.…”

Recent advances in natural polymer-based hydroxyapatite scaffolds: Properties and applications

“…Sintering temperature below 800 • C results in the scaffold with lowered mechanical strength but enhanced in vivo biodegradability, which could be due to the presence of other forms of calcium phosphate in the ceramic graft; HAP possesses the least bio-degradability [16]. Since HAP is stable under an in vivo environment, it is commonly endorsed as a coating material for metallic implants (generally titanium and stainless alloys) to modify the surface morphological characteristics and formulate the implant to further enhance the biological activity [17].…”