Cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3

Sun, Connie; Xiong, Likuan; Yao, Chengcai; Mi, Y.; Hu, Yawen; Hu, Likuan

doi:10.1109/iembs.2002.1137026

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…In our past experiments, we found that the selfdeveloped steep pulser made undoubted killing effect on tumor tissue in vitro and in vivo, and we roughly considered and analyzed possible mechanism as irreversible electrical breakdown (IREB) [1][2][3]. Moreover, it had been proved that steep pulse electric field (SPEF) could prolong the survival period of tumor-bearing BALB/c mice, its inhibiting and lethal effect on tumor cell made it possible for SPEF to be used in clinical application of tumor treatment.…”

Section: Introductionmentioning

confidence: 99%

Vascular effect and immunity effect of steep pulse electric field on Walker 256-bearing Wistar mice

Xiong

Sun

Yao

et al.

The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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The vascular effect and immunity effect of SPEF on Wistar mice were studied, and the possible treatment mechanisms and clinical trial methods of SPEF were discussed. We tested lymphocyte proliferation ratio and activity of NK cell, and the contents of IFN-alpha and IL-4 in the serum lymphocyte were inspected with ELISA method. Moreover, macrophage were collected, the content of TNF-alpha excreted by macrophage were inspected with ELISA method, too. Statistical analysis was performed using the SPSS10.0 software package and t-test. VEGF level in test group was significantly decreased, and VEGF expression was weakened evidently. SPEF strengthened activity of NK cell, enlarged quantities of TNF-alpha from test group, accelerated exudation of TH1 cell factor and slowed exudation of TH2 cell factor. SPEF showed effective anti-tumor effect through restraining the formation of fresh vessels and baffling the birth and transfer of tumor cell. It could strengthen organism immunity function, and had visible effect on Walker-256 tumor.

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Section: Introductionmentioning

confidence: 99%

Vascular effect and immunity effect of steep pulse electric field on Walker 256-bearing Wistar mice

Xiong

Sun

Yao

et al.

The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

View full text Add to dashboard Cite

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Lethal and inhibitory effects of steep pulsed electric field on tumor-bearing BALB/c mice

Sun

Yao

et al.

The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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Exposing cancer cell to steep pulsed electric field (SPEF) with appropriate dosage, the cell may die due to irreversible electrical breakdown (IREB). 48 BALB/c mice inoculated with U/sub 14/ cervical cancer cell were treated with SPEF to study the lethal and inhibitory effects of SPEF on them. They were randomly divided into test group (26) and control group (22), and then four experiments were carried out. Histological observation and ultrastructural observation showed that SPEF destroyed the integrality of tumor cell irreversibly with pyknosis, karyoclasis and karyolysis. Tumor volume measurement indicated that SPEF significantly slowed down the growth speed of tumor. In the experiment of survival time investigation, the average survival time of control group and test group was 33.03 days and no less than 52.05 days respectively, which confirmed that SPEF prolonged the survival time of tumor-bearing mice obviously. With further experimental study and mechanism analysis, this technique is quite possible to be an effective physiotherapy for tumor treatment.

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Cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3

Cited by 2 publications

References 3 publications

Vascular effect and immunity effect of steep pulse electric field on Walker 256-bearing Wistar mice

Vascular effect and immunity effect of steep pulse electric field on Walker 256-bearing Wistar mice

Lethal and inhibitory effects of steep pulsed electric field on tumor-bearing BALB/c mice

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