Cytochrome P450 Monooxygenases CYP6AY3 and CYP6CW1 Regulate Rice Black-Streaked Dwarf Virus Replication in Laodelphax striatellus (Fallén)

Zhang, Jianhua; Zhao, Ming; Zhou, Yijun; Xu, Qiufang; Yang, Yuanxue

doi:10.3390/v13081576

Cited by 8 publications

(9 citation statements)

References 35 publications

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“…( Liu et al, 2020 ; Pandian et al, 2020 ). Previous studies indicated that several P450 genes were involved in disease resistance in rice and barley ( Ameen et al, 2021 ; Zhang et al, 2021 ; Wang A. et al, 2022 ), implying their potential roles in maize disease resistance. Interestingly, we also found that 8 of 267 (4 of 115) co-DEGs belong to the ABC transporter superfamily.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome meta-analysis reveals a set of genes involved in the responses to multiple pathogens in maize

Wang

Sun

et al. 2022

Front. Plant Sci.

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Maize production is constantly threatened by the presence of different fungal pathogens worldwide. Genetic resistance is the most favorable approach to reducing yield losses resulted from fungal diseases. The molecular mechanism underlying disease resistance in maize remains largely unknown. The objective of this study was to identify key genes/pathways that are consistently associated with multiple fungal pathogen infections in maize. Here, we conducted a meta-analysis of gene expression profiles from seven publicly available RNA-seq datasets of different fungal pathogen infections in maize. We identified 267 common differentially expressed genes (co-DEGs) in the four maize leaf infection experiments and 115 co-DEGs in all the seven experiments. Functional enrichment analysis showed that the co-DEGs were mainly involved in the biosynthesis of diterpenoid and phenylpropanoid. Further investigation revealed a set of genes associated with terpenoid phytoalexin and lignin biosynthesis, as well as potential pattern recognition receptors and nutrient transporter genes, which were consistently up-regulated after inoculation with different pathogens. In addition, we constructed a weighted gene co-expression network and identified several hub genes encoding transcription factors and protein kinases. Our results provide valuable insights into the pathways and genes influenced by different fungal pathogens, which might facilitate mining multiple disease resistance genes in maize.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome meta-analysis reveals a set of genes involved in the responses to multiple pathogens in maize

Wang

Sun

et al. 2022

Front. Plant Sci.

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“…Moreover, the degradation products of chlorophyll metabolism would serve antiviral function when binding to their receptor proteins ( 56 ). A recent study shows that the p450 related drug metabolism pathways can regulate the replication of plant virus in insect ( 57 ). In vertebrates, retinoids play key roles in the process of embryonic development and tissue regeneration ( 58 , 59 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Densovirus in Aphid, and Uncovering the Possible Antiviral Process During Its Infection

et al. 2022

Front. Immunol.

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Densoviruses (DVs) are single-stranded DNA viruses and exclusively happen in invertebrates. Most of DVs reported in insects are pathogenic to their native hosts, however, no pathogenic effect of them has been examined in vertebrates. Hence, DVs are the potential agents used in pest managements. Aphids are the primary vectors of plant viruses. In this study, we identified a novel DV in Chinese Sitobion miscanthi population, provisionally named “Sitobion miscanthi densovirus” (SmDV). Taxonomically, SmDV belongs to genus Hemiambidensovirus. In S. miscanthi, SmDV is hosted in diverse cells and can be horizontally transmitted via wheat feeding. Subject to SmDV, aphids activate their intrinsic antiviral autophagy pathway. Grouped with ascorbate and aldarate metabolism, chlorophyll metabolism, p450 related drug metabolism, and retinoid metabolism, aphids form a complex immune network response to the infection of SmDV. Obviously, it works as elder aphids still alive even they contain the highest examined concentration of SmDV. This study provides a foundation for the identifications of novel DVs, and further improves the understanding of the molecular interactions between insects and DVs.

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“…RNA interference (RNAi) pathway, including the small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA pathway, play important role in antiviral infection in animal and plant hosts. 10-14 14 The miRNAs are non-coding RNAs with 19-25 nucleotides that negatively regulate gene expression by inhibition the expression of messenger RNA (mRNA). 10 However, the mechanism of insect vector miRNAs modulate plant virus infection remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

“…16,17 RBSDV infection in L. striatellus could modulate the changes of miRNAs expression. 14,[18][19][20] However, the specific interaction mechanism of RBSDV with miRNAs is largely unknown. The miR184-3p was one of the most abundantly expressed miRNAs, and was up-regulated in RBSDV infected L. striatellus.…”

Section: Introductionmentioning

confidence: 99%

The miR184‐3p targets neuron‐specific ecdysone inducible protein 78 to promote rice black streaked dwarf virus propagation in its planthopper vector

Wang,

Dong,

Wang

et al. 2024

Pest Management Science

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BackgroundMicroRNAs (miRNAs) are non‐coding RNAs that play a pivotal role in antiviral infection. The miR184‐3p has been identified to promote rice black streaked dwarf virus (RBSDV) infection in vector Laodelphax striatellus, whether it targets other genes of L. striatellus to modulate RBSDV propagation remains unknown.ResultsWe first analyzed the expression profiles of miR184‐3p and its role in regulating RBSDV infection in L. striatellus. Then the candidate genes expression of miR184‐3p were systemically analyzed with gain and loss function of miR184‐3p, and the interaction of candidate gene, ecdysone inducible protein 78 (Eip78) with miR184‐3p was verified by dual luciferase reporter assay. We found Eip78 is evolutionary conserved among agricultural pests and predominantly expressed in the central nervous system (CNS) of L. striatellus. Knockdown of Eip78 effectively increased RBSDV propagation and transmission. Blockade with Eip78 antibody or injection with Eip78 protein could significantly regulate RBSDV infection. Further analysis revealed that knockdown of Eip78 specifically suppresses RBSDV infection in the head part but not in the body part of L. striatellus. Besides, knockdown of ecdysone receptor (EcR) notably restricted Eip78 expression and increased RBSDV accumulation in L. striatellus.ConclusionsTaken together, we identified a novel target gene of miR184‐3p, Eip78, a member of the ecdysone signaling pathway, and revealed the anti‐RBSDV role of Eip78 in the CNS of L. striatellus. These results shed light on the interaction mechanisms of miRNAs, virus and ecdysone signaling pathway in insect vector.This article is protected by copyright. All rights reserved.

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Cytochrome P450 Monooxygenases CYP6AY3 and CYP6CW1 Regulate Rice Black-Streaked Dwarf Virus Replication in Laodelphax striatellus (Fallén)

Cited by 8 publications

References 35 publications

Comparative transcriptome meta-analysis reveals a set of genes involved in the responses to multiple pathogens in maize

Comparative transcriptome meta-analysis reveals a set of genes involved in the responses to multiple pathogens in maize

Identification of a Novel Densovirus in Aphid, and Uncovering the Possible Antiviral Process During Its Infection

The miR184‐3p targets neuron‐specific ecdysone inducible protein 78 to promote rice black streaked dwarf virus propagation in its planthopper vector

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