Cytochrome P450 Monooxygenases as a Tool for Metabolizing of Herbicides in Plants

Gorinova, N.; Nedkovska, M.; Atanassov, A.

doi:10.1080/13102818.2005.10817290

Cited by 12 publications

(9 citation statements)

References 34 publications

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“…Perera et al [ 71 ] showed that transgenic Arabidopsis plants expressing the type 1 inositol-1,4,5-trisphosphate 5-phosphatase had enhanced drought tolerance and altered ABA signaling. Cytochrome P450s (CytoP450s), a superfamily of ubiquitous heme-containing mixed-function monooxygenases proteins, play a key role in biotic and abiotic stresses [ 72 ]. In Arabidopsis , at least five of the 29 CytoP450s are induced by abiotic and biotic stresses including Alternaria brassicicola or Alternaria alternata , paraquat, rose bengal, UV-C stress, heavy metal stress (CuSO 4 ), mechanical wounding, drought, high salinity, low temperature or hormones [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis , at least five of the 29 CytoP450s are induced by abiotic and biotic stresses including Alternaria brassicicola or Alternaria alternata , paraquat, rose bengal, UV-C stress, heavy metal stress (CuSO 4 ), mechanical wounding, drought, high salinity, low temperature or hormones [ 73 ]. Transgenic tobacco and potato plants expressing CytP450 with increased monooxygenase activity tolerated better oxidative stress after herbicide treatment [ 72 ]. Therefore, the observed higher level of putative type 1 inositol-1,4,5-trisphosphate 5-phosphatase CVP2 (L43, Table 1 ) and putative CytoP450 (L19, Table 1 ) might contribute to Mg-deficiency tolerance of plants.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng

Lee

et al. 2015

BMC Genomics

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BackgroundMagnesium (Mg)-deficiency is frequently observed in Citrus plantations and is responsible for the loss of productivity and poor fruit quality. Knowledge on the effects of Mg-deficiency on upstream targets is scarce. Seedlings of ‘Xuegan’ [Citrus sinensis (L.) Osbeck] were irrigated with Mg-deficient (0 mM MgSO4) or Mg-sufficient (1 mM MgSO4) nutrient solution for 16 weeks. Thereafter, we first investigated the proteomic responses of C. sinensis roots and leaves to Mg-deficiency using two-dimensional electrophoresis (2-DE) in order to (a) enrich our understanding of the molecular mechanisms of plants to deal with Mg-deficiency and (b) understand the molecular mechanisms by which Mg-deficiency lead to a decrease in photosynthesis.ResultsFifty-nine upregulated and 31 downregulated protein spots were isolated in Mg-deficient leaves, while only 19 upregulated and 12 downregulated protein spots in Mg-deficient roots. Many Mg-deficiency-responsive proteins were involved in carbohydrate and energy metabolism, followed by protein metabolism, stress responses, nucleic acid metabolism, cell wall and cytoskeleton metabolism, lipid metabolism and cell transport. The larger changes in leaf proteome versus root one in response to Mg-deficiency was further supported by our observation that total soluble protein concentration was decreased by Mg-deficiency in leaves, but unaffected in roots. Mg-deficiency had decreased levels of proteins [i.e. ribulose-1,5-bisphosphate carboxylase (Rubisco), rubisco activase, oxygen evolving enhancer protein 1, photosynthetic electron transfer-like protein, ferredoxin-NADP reductase (FNR), aldolase] involved in photosynthesis, thus decreasing leaf photosynthesis. To cope with Mg-deficiency, C. sinensis leaves and roots might respond adaptively to Mg-deficiency through: improving leaf respiration and lowering root respiration, but increasing (decreasing) the levels of proteins related to ATP synthase in roots (leaves); enhancing the levels of proteins involved in reactive oxygen species (ROS) scavenging and other stress-responsive proteins; accelerating proteolytic cleavage of proteins by proteases, protein transport and amino acid metabolism; and upregulating the levels of proteins involved in cell wall and cytoskeleton metabolism.ConclusionsOur results demonstrated that proteomics were more affected by long-term Mg-deficiency in leaves than in roots, and that the adaptive responses differed between roots and leaves when exposed to long-term Mg-deficiency. Mg-deficiency decreased the levels of many proteins involved in photosynthesis, thus decreasing leaf photosynthesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1462-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng

Lee

et al. 2015

BMC Genomics

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“…We found that CytoP45 0 (TDF #137_2) was repressed in excess B-treated C. grandis roots, as obtained on B-toxic Arabidopsis roots (Aquea et al, 2012 ). Transgenic potato and tobacco plants expressing CytoP450 displayed increased monooxygenase activity and enhanced tolerance to oxidative stress after herbicide treatment (Gorinova et al, 2005 ). Therefore, B-excess-induced down-regulation of AOX and CytoP450 in C. grandis roots might contribute to the less B-tolerance of C. grandis .…”

Section: Discussionmentioning

confidence: 99%

Long-Term Boron-Excess-Induced Alterations of Gene Profiles in Roots of Two Citrus Species Differing in Boron-Tolerance Revealed by cDNA-AFLP

Guo

Yang

et al. 2016

Front. Plant Sci.

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Boron (B) toxicity is observed in some citrus orchards in China. However, limited data are available on the molecular mechanisms of citrus B-toxicity and B-tolerance. Using cDNA-AFLP, we identified 20 up- and 52 down-regulated genes, and 44 up- and 66 down-regulated genes from excess B-treated Citrus sinensis and Citrus grandis roots, respectively, thereby demonstrating that gene expression profiles were more affected in the latter. In addition, phosphorus and total soluble protein concentrations were lowered only in excess B-treated C. grandis roots. Apparently, C. sinensis had higher B-tolerance than C. grandis. Our results suggested that the following several aspects were responsible for the difference in the B-tolerance between the two citrus species including: (a) B-excess induced Root Hair Defective 3 expression in C. sinensis roots, and repressed villin4 expression in C. grandis roots; accordingly, root growth was less inhibited by B-excess in the former; (b) antioxidant systems were impaired in excess B-treated C. grandis roots, hence accelerating root senescence; (c) genes related to Ca2+ signals were inhibited (induced) by B-excess in C. grandis (C. sinensis) roots. B-excess-responsive genes related to energy (i.e., alternative oxidase and cytochrome P450), lipid (i.e., Glycerol-3-phosphate acyltransferase 9 and citrus dioxygenase), and nucleic acid (i.e., HDA19, histone 4, and ribonucleotide reductase RNR1 like protein) metabolisms also possibly accounted for the difference in the B-tolerance between the two citrus species. These data increased our understanding of the mechanisms on citrus B-toxicity and B-tolerance at transcriptional level.

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“…The expression of cytochrome P450s ( CytoP450s ) was increased in Vicia sativa seedlings when exposed to plant hormone methyl jasmonate (Pinot et al, 1998 ) and in B-toxic C. grandis leaves (Guo et al, 2014 ). Transgenic tobacco and potato plants over-expressing CytoP450 displayed increased monooxygenase activity and enhanced tolerance of oxidative stress after herbicide treatment (Gorinova et al, 2005 ). Therefore, the up-regulation of CytoP450 (TDF #R132-6a) in B-deficient roots (Table 1 ) might contribute to the tolerance of plants to B-deficiency.…”

Section: Discussionmentioning

confidence: 99%

Long-term boron-deficiency-responsive genes revealed by cDNA-AFLP differ between Citrus sinensis roots and leaves

Yang

et al. 2015

Front. Plant Sci.

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Seedlings of Citrus sinensis (L.) Osbeck were supplied with boron (B)-deficient (without H3BO3) or -sufficient (10 μM H3BO3) nutrient solution for 15 weeks. We identified 54 (38) and 38 (45) up (down)-regulated cDNA-AFLP bands (transcript-derived fragments, TDFs) from B-deficient leaves and roots, respectively. These TDFs were mainly involved in protein and amino acid metabolism, carbohydrate and energy metabolism, nucleic acid metabolism, cell transport, signal transduction, and stress response and defense. The majority of the differentially expressed TDFs were isolated only from B-deficient roots or leaves, only seven TDFs with the same GenBank ID were isolated from the both. In addition, ATP biosynthesis-related TDFs were induced in B-deficient roots, but unaffected in B-deficient leaves. Most of the differentially expressed TDFs associated with signal transduction and stress defense were down-regulated in roots, but up-regulated in leaves. TDFs related to protein ubiquitination and proteolysis were induced in B-deficient leaves except for one TDF, while only two down-regulated TDFs associated with ubiquitination were detected in B-deficient roots. Thus, many differences existed in long-term B-deficiency-responsive genes between roots and leaves. In conclusion, our findings provided a global picture of the differential responses occurring in B-deficient roots and leaves and revealed new insight into the different adaptive mechanisms of C. sinensis roots and leaves to B-deficiency at the transcriptional level.

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Cytochrome P450 Monooxygenases as a Tool for Metabolizing of Herbicides in Plants

Cited by 12 publications

References 34 publications

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Long-Term Boron-Excess-Induced Alterations of Gene Profiles in Roots of Two Citrus Species Differing in Boron-Tolerance Revealed by cDNA-AFLP

Long-term boron-deficiency-responsive genes revealed by cDNA-AFLP differ between Citrus sinensis roots and leaves

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