Cytochrome P450 Metabolites of Arachidonic Acid in Human Placenta

Schaefer, W.R.; Werner, Karin; Schweer, Horst; Schneider, Joanne Kraenzle; Arbogast, Eva; Zahradnik, H. P.

doi:10.1016/s0090-6980(97)00148-2

Cited by 16 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike previous reports in mammalian placenta (Schaefer et al, 1997), 11,12-DHET and 14,15-DHET did not change with respect to embryonic development in the alligator CAM. Oxidized products of linoleic acid (13-HODE and 9-HODE) did increase with development in the alligator CAM; however, levels of these epoxyeicosatrienoic acids are unknown within the mammalian placenta during pregnancy – though it is known that the placenta has the required enzymatic machinery to secrete these oxidized lipids (Fang et al, 1999; Fournier et al, 2011).…”

Section: Discussioncontrasting

confidence: 99%

Alterations in eicosanoid composition during embryonic development in the chorioallantoic membrane of the American alligator (Alligator mississippiensis) and domestic chicken (Gallus gallus)

Cantu

Bowden

Scott

et al. 2016

General and Comparative Endocrinology

View full text Add to dashboard Cite

Eicosanoids are signaling lipids known to regulate several physiological processes in the mammalian placenta, including the initiation of parturition. Though all amniotes construct similar extraembryonic membranes during development, the composition and function of eicosanoids in extraembryonic membranes of oviparous reptiles is largely unknown. The majority of effort placed in eicosanoid investigations is typically targeted toward defining the role of specific compounds in disease etiology; however, comprehensive characterization of several pathways in eicosanoid synthesis during development is also needed to better understand the complex role of these lipids in comparative species. To this end, we have examined the chorioallantoic membrane (CAM) of the American alligator (Alligator mississippiensis) and domestic chicken (Gallus gallus) during development. Previously, our lab has demonstrated that the CAM of several oviparous species shared conserved steroidogenic activity, a feature originally attributed to mammalian amniotes. To further explore this, we have developed a liquid chromatography/tandem mass spectrometry method that is used here to quantify multiple eicosanoids in the CAM of two oviparous species at different stages of development. We identified 18 eicosanoids in the alligator CAM; the cyclooxygenase (COX) pathway showed the largest increase from early development to later development in the alligator CAM. Similarly, the chicken CAM had an increase in COX products and COX activity, which supports the LC-MS/MS analyses. Jointly, our findings indicate that the CAM tissue of an oviparous species is capable of eicosanoid synthesis, which expands our knowledge of placental evolution and introduces the possibility of future comparative models of placental function.

show abstract

Section: Discussioncontrasting

confidence: 99%

Alterations in eicosanoid composition during embryonic development in the chorioallantoic membrane of the American alligator (Alligator mississippiensis) and domestic chicken (Gallus gallus)

Cantu

Bowden

Scott

et al. 2016

General and Comparative Endocrinology

View full text Add to dashboard Cite

show abstract

“…EETs are mainly generated in the liver, kidney and vascular endothelium 5 . In addition to circulating levels of EETs, they are formed in the placenta, trophoblast, amnion, chorion, decidua, and myometrium of the gravid uterus [28][29][30] . Therefore in recent years their contribution to physiological response to normal pregnancy and the pathophysiology of pregnancy induced hypertension is pointed out more extensively.…”

Section: Resultsmentioning

confidence: 99%

“…CYP2C and CYP2J families of CYP epoxygenases convert AA to four biologically active EETs (5, 6-EET, 8, 9-EET, 11, 12-EET, and 14, 15-EET) that have vasoprotective, antihypertensive, antiimflammatory and profibrinolytic effects [25][26][27] . In addition to circulating levels of EETs, they are formed in the placenta, trophoblast, amnion, chorion, decidua, and myometrium of the gravid uterus [28][29][30] . Growing evidence suggest that EETs' contribute to the physiological response to normal pregnancy and the pathophysiology of pregnancy induced hypertension.…”

Section: Introductionmentioning

confidence: 99%

Epoxyeicosatrienoic acid Metabolism in Preeclampsia

Sarı¹,

Pınarbaşı

Yıldız

2018

Cumhuriyet Medical Journal

View full text Add to dashboard Cite

Preeclampsia (PE) is a disease that characterized by hypertension and proteinuria during pregnancy. Epoxyeicosatrienoic acids (EETs) are arachidonic acid metabolites which have vasodilatator, anti-inflammatory and profibrinolytic effects. Soluble epoxide hydrolase (sEH; EC 3.3.3.2) catalyses the degradation of EETs to their inactive diols (DHETs). Low circulating levels of EETs may be related to high blood pressure in preeclampsia. The aim of this study is to determine the level of 11,12-DHETs, a representative metabolite of sEH-mediated metabolism of EET, in preeclamptic patients. Method: 11,12-DHET levels were measured by ELISA in plasma samples of 75 PE patients and 75 normotensive pregnant women as controls. Results: It was found that lasma 11,12-DHET levels of PE patients was significantly increased compared to the control group (p <0.05). Conclusions: These results and our previous findings suggest that high sEH activities in PE patients may cause to produce more 11,12-DHETs in PE. sEH enzyme with high catalytic activity may play a role in the pathogenesis of PE by contributing to the reduction of vasodilatator, anti-hypertensive and anti-inflammatory effects of EETs by rapid degradation of these molecules.

show abstract

“…These EETs affect ion-channel activity in cardiac myocytes and the contractility of the heart, and this might be one of the mechanisms by which these epoxides contribute to heart protection during cardiac ischemia [46]. The epoxygenase pathway of the arachidonic acid cascade has been demonstrated in human placenta [47] and could explain the CYP2J2 expression observed in this tissue. Finally, CYP2J2 expressed in skeletal muscle could produce EETs and therefore activate Ca 2 + -activated K + channels in smooth muscle cells [48].…”

Section: Discussionmentioning

confidence: 96%