Cytochrome <i>c</i> at Model Membrane Surfaces:  Exploration via Second Harmonic Generation-Circular Dichroism and Surface-Enhanced Resonance Raman Spectroscopy

Petralli-Mallow, Teresa; Plant, Anne L.; Lewis, and M. L.; Hicks, Janice M.

doi:10.1021/la9913250

Cited by 45 publications

(28 citation statements)

References 36 publications

(83 reference statements)

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“…SHG-CD has also been used, together with a surface-enhanced resonance Raman study, to monitor the oxidation state of cytochrome c adsorbed on model membranes surfaces. 72 SHG-CD was shown to be a particularly sensitive probe of the redox state of the heme group, which is known to be essential for the electron transfer process that occurs in cytochrome c. Simpson and co-workers used chiral SHG experiments to observe the dynamics of protein binding (bovine serum albumin) to a surface. 73 Their experimental results are shown in Figure 7.…”

Section: Surface Nonlinear Opticsmentioning

confidence: 99%

Nonlinear optical spectroscopy of chiral molecules

2005

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We review nonlinear optical processes that are specific to chiral molecules in solution and on surfaces. In contrast to conventional natural optical activity phenomena, which depend linearly on the electric field strength of the optical field, we discuss how optical processes that are nonlinear (quadratic, cubic, and quartic) functions of the electromagnetic field strength may probe optically active centers and chiral vibrations. We show that nonlinear techniques open entirely new ways of exploring chirality in chemical and biological systems: The cubic processes give rise to nonlinear circular dichroism and nonlinear optical rotation and make it possible to observe dynamic chiral processes at ultrafast time scales. The quadratic second-harmonic and sum-frequency-generation phenomena and the quartic processes may arise entirely in the electric-dipole approximation and do not require the use of circularly polarized light to detect chirality. They provide surface selectivity and their observables can be relatively much larger than in linear optical activity. These processes also give rise to the generation of light at a new color, and in liquids this frequency conversion only occurs if the solution is optically active. We survey recent chiral nonlinear optical experiments and give examples of their application to problems of biophysical interest.

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Section: Surface Nonlinear Opticsmentioning

confidence: 99%

Nonlinear optical spectroscopy of chiral molecules

2005

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“…Except for the modulation of cellular interactions, the orientation and conformation of proteins adsorbed onto a material surface is critical for cell integrins to recognize specific sites, which may initialize diverse signaling events. It has been widely believed that proteins undergo some conformational change when adsorbed on surfaces, with the help of sum frequency generation vibrational spectroscopy, time‐of‐flight secondary ion mass spectrometry, and second harmonic generation‐circular dichroism . It has been observed that proteins adsorbed on hydrophobic surfaces generally undergo structural rearrangement such that nonpolar amino acids interact preferentially with the surfaces, which gives rise to strong interfacial interactions and irreversible adsorption.…”

Section: Introductionmentioning

confidence: 99%

Titania nanotubes dimensions‐dependent protein adsorption and its effect on the growth of osteoblasts

Yang

Shen

et al. 2013

J Biomedical Materials Res

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In this study, we report the influence of titania nanotubes (TiNTs) dimensions on the adsorption of collagen (COL) and fibronectin (FN), and its subsequent effect on the growth of osteoblasts. TiNTs with different diameters of around 30 and 100 nm were prepared with anodization. The adsorption profiles of proteins and cell behaviors were evaluated using spectrophotometric measurement, immunofluorescence staining, cell viability, and cytoskeleton morphology, respectively. The results showed that although the growth of osteoblasts was highly sensitive to the dimensions TiNTs, the preadsorbed COL and FN could reduce the difference. Molecular dynamics (MD) simulation results confirmed that the main driving force for protein adsorption was the physical adsorption. The TiNTs with bigger dimensions had higher interaction energies, and thus leading to higher proteins (COL and FN) adsorption and obvious influences on cell behaviors. MD simulation revealed that the orientation and conformation of proteins adsorbed onto surfaces of TiNTs was critical for cell integrins to recognize specific sites. When FN molecules adsorbed onto the surfaces of TiNTs, their RGD (Arg-Gly-Asp) sites were easily exposed to outside and more likely to bond with the fibronectin receptors, in turn regulating the cellular behaviors.

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“…However, it can probe the chirality of anisotropic systems such as chiral surfaces, as first demonstrated by Hicks group [8,9,10,11,12]. Reviews on SHG as a probe for surface chirality can be found in references [13,14,15].…”

Section: Sum-frequency Generation As a Probe Of Chiralitymentioning

confidence: 99%

“…As a result, SFG has higher sensitivity and has been used to obtain optically active electronic and vibrational spectra of a monolayer of chiral molecules [19,23]. SHG, although forbidden in chiral liquids, has been shown by Hicks et al to be able to probe the chirality of a monolayer [8,9,10,11,12].…”

Section: Sum-frequency Vibrational Spectroscopy (Sfvs) Of Achiral Intmentioning

confidence: 99%

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Sum-Frequency Generation from Chiral Media and Interfaces

2006

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Professor Yuen-Ron Shen, Co-chair Professor Richard J. Saykally, Co-chair Sum frequency generation (SFG), a second-order nonlinear optical process, is electric-dipole forbidden in systems with inversion symmetry. As a result, it has been used to study chiral media and interfaces, systems intrinsically lacking inversion symmetry.This thesis describes recent progresses in the applications of and new insights into SFG from chiral media and interfaces.SFG from solutions of chiral amino acids is investigated, and a theoretical model explaining the origin and the strength of the chiral signal in electronic-resonance SFG spectroscopy is discussed. An interference scheme that allows us to distinguish enantiomers by measuring both the magnitude and the phase of the chiral SFG response is described, as well as a chiral SFG microscope producing chirality-sensitive images with sub-micron 2 resolution. Exploiting atomic and molecular parity nonconservation, the SFG process is also used to solve the Ozma problems.Sum frequency vibrational spectroscopy is used to obtain the adsorption behavior of leucine molecules at air-water interfaces. With poly(tetrafluoroethylene) as a model system, we extend the application of this surface-sensitive vibrational spectroscopy to fluorinecontaining polymers.

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Cytochrome c at Model Membrane Surfaces: Exploration via Second Harmonic Generation-Circular Dichroism and Surface-Enhanced Resonance Raman Spectroscopy

Cited by 45 publications

References 36 publications

Nonlinear optical spectroscopy of chiral molecules

Nonlinear optical spectroscopy of chiral molecules

Titania nanotubes dimensions‐dependent protein adsorption and its effect on the growth of osteoblasts

Sum-Frequency Generation from Chiral Media and Interfaces

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