Pushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the ‘Janelia Fluor’ (JF) series of dyes. Here, we refine and extend this strategy, showing that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties with unprecedented precision. This strategy yields a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red with utility in cells, tissue, and animals.
Phase-sensitive sum-frequency spectroscopy provides correct characterization of vibrational resonances of water-vapor interfaces and allows better identification of interfacial water species contributing to different parts of the spectra. Iodine ions emerging at an interface create a surface field that tends to reorient the more loosely bonded water molecules below the topmost layer.
Biological specimens are rife with optical inhomogeneities that seriously degrade imaging performance under all but the most ideal conditions. Measuring and then correcting for these inhomogeneities is the province of adaptive optics. Here we introduce an approach to adaptive optics in microscopy wherein the rear pupil of an objective lens is segmented into subregions, and light is directed individually to each subregion to measure, by image shift, the deflection faced by each group of rays as they emerge from the objective and travel through the specimen toward the focus. Applying our method to two-photon microscopy, we could recover near-diffraction-limited performance from a variety of biological and nonbiological samples exhibiting aberrations large or small and smoothly varying or abruptly changing. In particular, results from fixed mouse cortical slices illustrate our ability to improve signal and resolution to depths of 400 microm.
Phase-sensitive sum-frequency vibrational spectroscopy was used to study water/vapor interfaces of HCl, HI, and NaOH solutions. The measured imaginary part of the surface spectral responses provided direct characterization of OH stretch vibrations and information about net polar orientations of water species contributing to different regions of the spectrum. We found clear evidence that hydronium ions prefer to emerge at interfaces. Their OH stretches contribute to the "ice-like" band in the spectrum. Their charges create a positive surface field that tends to reorient water molecules more loosely bonded to the topmost water layer with oxygen toward the interface, and thus enhances significantly the "liquid-like" band in the spectrum. Iodine ions in solution also like to appear at the interface and alter the positive surface field by forming a narrow double-charge layer with hydronium ions. In NaOH solution, the observed weak change of the "liquid-like" band and disappearance of the "ice-like" band in the spectrum indicates that OH-ions must also have excess at the interface. How they are incorporated in the interfacial water structure is however not clear.
The past quarter century has witnessed rapid developments of fluorescence microscopy techniques that enable structural and functional imaging of biological specimens at unprecedented depth and resolution. The performance of these methods in multicellular organisms, however, is degraded by sample-induced optical aberrations. Here I review recent work on incorporating adaptive optics, a technology originally applied in astronomical telescopes to combat atmospheric aberrations, to improve image quality of fluorescence microscopy for biological imaging.
Recently, sum-frequency spectroscopy has become an indispensable tool for many interfacial studies because of its high surface specificity. Sum-frequency vibrational spectroscopy (SFVS), in particular, is the only technique available to probe the vibrational structures of a host of different interfaces. 1 As a surface-specific coherent nonlinear optical process, the SFVS output is proportional to the absolute square of the surface response coefficient, |χ S(2) | 2 , which is resonantly enhanced as one of the input frequencies scans over vibrational resonances, thus yielding a surface vibrational spectrum. However, χ S(2) is complex in general, and for complete information, we need to know both the amplitude (|χ S (2) |) and the
Understanding the functions of a brain region requires knowing the neural
representations of its myriad inputs, local neurons, and outputs. Primary visual
cortex (V1) has long been thought to compute visual orientation from untuned
thalamic inputs, but very few thalamic inputs have been measured in any mammal.
We determined the response properties of ~28,000 thalamic boutons and
~4,000 cortical neurons in layers 1–5 of awake mouse V1. With
adaptive optics allowing accurate measurement of bouton activity deep in cortex,
we found that around half of the boutons in the main thalamorecipient L4 carry
orientation-tuned information, and their orientation/direction biases are also
dominant in the L4 neuron population, suggesting that these neurons may inherit
their selectivity from tuned thalamic inputs. Cortical neurons in all layers
exhibited sharper tuning than thalamic boutons and a greater diversity of
preferred orientations. Our results provide data-rich constraints for refining
mechanistic models of cortical computation.
Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.
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