Cytochrome c oxidase subunit II mRNA levels during T-lymphocyte proliferation and liver regeneration

Otero, Alejandro González; Bustelo, Xosé R.; Gómez-Márquez, Jaime

doi:10.1016/0167-4889(91)90155-q

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…T cells and CD4? It is over expressed during proliferation of thymocytes [44] and also in human mammary epithelial cells regulated by heregulin b1 [45]. Other microarray studies have identified many host pro-apoptotic genes to be upregulated in CD4?…”

Section: Discussionmentioning

confidence: 99%

Differential modulation of mitochondrial OXPHOS system during HIV-1 induced T-cell apoptosis: up regulation of Complex-IV subunit COX-II and its possible implications

Tripathy

Mitra

2009

Apoptosis

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Human Immunodeficiency Virus-1 (HIV-1) infection leads to CD4+ T cell depletion primarily by apoptosis employing both intrinsic and extrinsic pathways. Although extensive literature exists about the role of mitochondrial proteins in HIV induced T cell apoptosis, there is little understanding about the role of different components of mitochondrial oxidative phosphorylation (OXPHOS) system in apoptosis. The OXPHOS system comprises of five enzyme complexes (Complex I, II, III, IV, V), subunits of which have been implicated in various functions in addition to their primary role in energy generating process. Here using differential gene expression analysis, we report that Cytochrome Oxidase-II (COX-II), a subunit of Complex-IV is induced in HIV infected apoptotic T-cells. We also observe a temporal up regulation of this subunit across different T-cell lines and in human PBMCs. Further analysis indicates increase in expression of majority of Complex-IV subunits with concomitant increase in Complex-IV activity in HIV infected T cells. Silencing of COX-II expression leads to reduced apoptosis in infected T-cells, indicating its importance in apoptosis. Furthermore, our results also show that the activities of enzyme complexes I, II and III are decreased while those of Complex IV and V are increased at the time of acute infection and apoptosis. This differential regulation in activities of OXPHOS system complexes indicate a complex modulation of host cell energy generating system during HIV infection that ultimately leads to T cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Differential modulation of mitochondrial OXPHOS system during HIV-1 induced T-cell apoptosis: up regulation of Complex-IV subunit COX-II and its possible implications

Tripathy

Mitra

2009

Apoptosis

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“…It has been shown that in certain cell types COXII mRNA levels are correlated with cell proliferation. For example, COXII mRNA levels increase during thymocyte proliferation and decrease when they are growth arrested (Otero et al, 1991). However, such correlationships seem not to exist in other cell types; the expression of COXII in splenic T‐cells and liver cells remains constant regardless of their proliferative status (Otero et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…For example, COXII mRNA levels increase during thymocyte proliferation and decrease when they are growth arrested (Otero et al, 1991). However, such correlationships seem not to exist in other cell types; the expression of COXII in splenic T‐cells and liver cells remains constant regardless of their proliferative status (Otero et al, 1991). Thus, although regulation of COXII expression is dependent on ERK or PI3‐K activation, changes of COXII expression may be independent of cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Induction or suppression of expression of cytochrome C oxidase subunit II by heregulin β 1 in human mammary epithelial cells is dependent on the levels of ErbB2 expression

Sun

Lin

Zhu

et al. 2002

Journal Cellular Physiology

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The ErbB family of receptor kinases is composed of four members: epidermal growth factor receptor (EGFR/ErbB1), ErbB2/neu, ErbB3, and ErbB4. Amplification of the ErbB2/neu is found in about 30% of breast cancer patients and is associated with a poor prognosis. Heregulin (HRG) activates the ErbB2 via induction of heterodimerization with ErbB3 and ErbB4 receptors. With suppression subtractive hybridization, we demonstrated that the expression of cytochrome c oxidase subunit II (COXII) is HRG-responsive. Two nontransformed human mammary epithelial cell lines, the HB2 and the HB2(ErbB2) (the HB2 engineered to overexpress ErbB2), displayed an opposite response to HRG-mediated regulation. HRG upregulated mRNA expression of COXII in the HB2 cells, but suppressed COXII expression in the HB2(ErbB2) cells. A human breast cancer cell line (T47D), which expresses ErbB2 at a level similar to that of the HB2 cells, also responded to HRG by increasing COXII mRNA levels. Therefore, HRG regulation of COXII expression depends on the levels of ErbB2 expression. Furthermore, the expression of COXII was inversely correlated to the levels of ErbB2, i.e., the cells overexpressing ErbB2 exhibited lower COXII levels. HRG-evoked signal transduction differed between the cells with normal ErbB expression and the cells overexpressing ErbB2. The activation of both ERK and PI3-K was essential for HRG regulation of COXII, i.e., blockage of either pathway eliminated HRG-mediated alteration. This is the first report demonstrating that the expression of mitochondria-encoded COXII is HRG-responsive. The levels of ErbB2 expression are decisive for the diverse biological activities of HRG.

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“…It has been reported that cytochrome oxidase mRNA is higher in those tissues with an elevated level of oxidative metabolism under certain physiological conditions, i.e. fertilisation, neuronal activity, and cellular proliferation of mammalian T cells [32]. In this EST study, four ESTs (AU090921, AU091017, AU091029, and AU091148) were found to code for heat shock protein 90.…”

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confidence: 91%

“…Subtracted cDNA were used to screen the cDNA library of Japanese flounder leucocytes stimulated with Con A/PMA as probes. Briefly, a total 1920 randomly selected colonies from the cDNA library were double-spotted identically on 7·5 11·5 cm Hybond-N+ nylon membrane (Amersham Phamacia Biotech), and hybridised at 68 C with the [ - 32 P] dCTP random primed labelled subtracted probes in QuikHyb Hybridisation Solution (Stratagene, U.S.A.), then washed in 2 SSC/0·1% SDS, 1 SSC/0·1% SDS, and 0·1 SSC/0·1% SDS.…”

mentioning

confidence: 99%

Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder leucocytes stimulated with Con A/PMA

Nam

Hirono

Aoki

2003

Fish & Shellfish Immunology

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Cytochrome c oxidase subunit II mRNA levels during T-lymphocyte proliferation and liver regeneration

Cited by 5 publications

References 19 publications

Differential modulation of mitochondrial OXPHOS system during HIV-1 induced T-cell apoptosis: up regulation of Complex-IV subunit COX-II and its possible implications

Differential modulation of mitochondrial OXPHOS system during HIV-1 induced T-cell apoptosis: up regulation of Complex-IV subunit COX-II and its possible implications

Induction or suppression of expression of cytochrome C oxidase subunit II by heregulin β 1 in human mammary epithelial cells is dependent on the levels of ErbB2 expression

Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder leucocytes stimulated with Con A/PMA

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