Cytochemistry of Virus-Infected Plant Cells

Martelli, G. P.

doi:10.1007/978-3-642-75818-8_8

Cited by 2 publications

(2 citation statements)

References 41 publications

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“…The cells of the symptomatic plants contain quasi-spherical, Double-Membrane-Bound particles (DMPs) of 100-200 nm in diameter present in the cytoplasm (Ahn et al, 1996). Such bodies were originally observed by Bradfute et al (1970) in figs and other plants affected by mite transmitted diseases and later described in detail (Martelli and Russo, 1984;Martelli, 1991;Martelli et al, 1993). The consistent association of DMBs with mosaic symptoms and the results of molecular investigations strongly indicated that DMBs are particles of FMV (Elbeaino et al, 2006(Elbeaino et al, , 2007Elbeshehy and Elbeaino, 2011).…”

Section: Introductionmentioning

confidence: 76%

Partial Nucleotide Sequence of the Family Bunyaviridae Associated with a Mosaic-Diseased Fig. in North Egypt

Sallam¹,

Farag²,

Elbeshe³

et al. 2015

International J. of Virology

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Four different patterns of chlorotic blotches, mosaic, yellowish mottling and deformation were observed and collected in four fig-growing areas of North Egypt from cv. Sultany farms. Fig mosaic virus like symptoms were successful purified through transmission an eriophyid mite, Aceria ficus Cotte to healthy potted rooted cuttings grown under glasshouse conditions. Young leaves appearing typical symptoms caused by FMV. Electron microscope observations of thin-sectioned tissues from symptomatic leaves showed Double Membrane Bodies (DMBs) characteristic for FMV particles compared with symptomless leaves. Viral particles belong to family of Bunyaviridae. The RT-PCR performed on total RNA extracted from leaf tissue infected with the studied virus confirmed the specificity of the primers of RNA-dependent RNA polymerase (RdRp) gene for RNA-dependent RNA polymerase. The size of the PCR amplified fragment was 302 bp. The sequence and phylogenetic analysis indicated that the Egyptian FMV isolate was closely related to other FMV isolates, especially the Italian and Arkansas ones with 80%similarity. The genetic relationship between the RdRp gene for the Egyptian isolate of FMV with other FMV sequences on the Genbank was studied.

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Section: Introductionmentioning

confidence: 76%

Partial Nucleotide Sequence of the Family Bunyaviridae Associated with a Mosaic-Diseased Fig. in North Egypt

Sallam¹,

Farag²,

Elbeshe³

et al. 2015

International J. of Virology

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show abstract

“…He was also interested in the study of virus–host interactions at the fine structural level. He conducted a series of studies on the cytopathology of viral infections [ 11 , 12 ], the structure and nature of inclusion bodies [ 13 ], cellular sites of viral nucleic acids and protein synthesis [ 14 ] and sites of intracellular accumulation of viral particles [ 7 , 15 , 16 , 17 ]. He was also interested in virus epidemiology, particularly that of viruses transmitted through the soil by nematodes [ 18 , 19 , 20 ], closteroviruses transmitted by pseudococcid scale insects [ 21 , 22 ] and viruses transmitted through seed [ 23 , 24 , 25 ] and pollen [ 24 ].…”

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confidence: 99%