Cytochemistry and Electron Microscopy

Sabatini, David D.; Bensch, Klaus G.; Barrnett, Russell J.

doi:10.1083/jcb.17.1.19

Cited by 3,692 publications

(453 citation statements)

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“…One segment of each pair was inoculated with 12% neutral buffered formalin and the other with 3% phosphate-buffered glutaraldehyde [17]. When the last segment had been inoculated the piglet was killed without delay using pentobarbitone sodium, and the tied off segments were immediately removed and immersed in fixative.…”

Section: Methodsmentioning

confidence: 99%

Gnotobiotic Piglets Experimentally Infected with Neonatal Calf Diarrhoea Reovirus-Like Agent (Rotavirus)

et al. 1976

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Abstract. Gnotobiotic piglets were infected with a strain of rotavirus that had been isolated from a calf and passaged four times in piglets. The resulting disease was studied by light and electron microscopy at 21,44 and 68h after inoculation. In thesmall intestine infection caused desquamation of the epithelial cells of the villi resulting in severe stunting. There was severe damage to microvilli and accumulation of lipid within the cytoplasm. Virus particles were seen in epithelial cells covering the stunted villi. Infection also caused desquamation of the superficial epithelial cells of the stomach mucosa and of the epithelial cells of the bronchi, bronchioles, and alveoli of the lungs. The reovirus-like particles, which have been seen in faeces of young children with acute nonbacterial gastroenteritis, have been found to be morphologically indistinguishable from those of neonatal calf diarrhoea [8, 101. The calf and human viruses also appeared morphologically and antigenically distinct from both reoviruses and orbiviruses, and the term rotavirus has been proposed [7-91.Although the calf and human rotaviruses are morphologically indistinguishable, we were unable to infect calves with human virus. Piglets, however, are susceptible to infection with human virus [5] but do not become ill. We passed calf rotavirus serially five times in gnotobiotic piglets, and each passage produced profuse diarrhoea, depression and anorexia. Our serological studies have shown that pigs are naturally infected either with calf

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Section: Methodsmentioning

confidence: 99%

Gnotobiotic Piglets Experimentally Infected with Neonatal Calf Diarrhoea Reovirus-Like Agent (Rotavirus)

et al. 1976

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“…These were fixed in 6% glutaraldehyde (Sabatini, Bensch and Barrnett, 1963), post-fixed in osmium tetroxide (Palade, 1952), and embedded in Araldite (Durcupan ACM Fluka) according to Luft (1961). Uranyl acetate was used during dehydration (Watson, 1958) and lead citrate applied to the sections (Reynolds, 1963) For light microscopy, several formalinfixed paraffin-embedded blocks were made, some prepared from areas adjacent to those from which the specimens for electron microscopy had been chosen.…”

Section: Methodsmentioning

confidence: 99%

Human Peripheral Lung Tumours: Light and Electron Microscopic Correlation

Mollo¹,

Canese²,

Campobasso³

1973

Br J Cancer

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Thirteen human peripheral lung tumours have been studied in both light and electron microscopy. They were classified as epidermoid carcinoma, mucus-secreting cell adenocarcinoma, and alveolar cell adenocarcinoma, the latter made up of granular pneumocytes. Alveolar cell cancer, as defined by ultrastructural features, could assume different gross histological patterns in light microscopy, and therefore electron microscopy is required for its identification. Since neither squamous nor mucous metaplasia was observed in any alveolar cell tumour, it is tentatively suggested that all peripheral lung tumours which lack these features may be derived from granular pneumocytes, irrespective of whether they appear to be adenocarcinomata or large cell carcinomata when examined by light microscopy. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig. 10 Fig. 11 Fig. 12 Fig. 13 Fig. 14

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“…After cutting into small bits, the specimens were fixed for two hours in ice cold 2% osmium tetroxide buffered at pH 7.4 with phosphate butter (MILLONIG 1961). Other specimens were fixed for 2hrs in 5% glutaraldehyde buffered at pH 7.4 with a cacodylate buffer (SABATINI, et al, 1963) and then, postfixed for two hrs in buffered 2% osmium tetroxide. After dehydration in graded ethoanol, the specimens were embedded in Epon epoxy resin (LUFT, 1961).…”

mentioning

confidence: 99%