Cytochemical localization of peroxidase activity in normal, proliferating and neoplastic thyroid tissues of rats. An ultrastructural study

Christov, Konstantin; Stoichkova, N

doi:10.1016/s0065-1281(77)80137-2

Cited by 6 publications

(2 citation statements)

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“…Thyroid peroxidase (TPO), which is essential for iodination of tyrosine and coupling of iodotyrosine in the follicle, is known to be significantly decreased in its activity in tumorigenesis (4,24) in association with decreased immunostainability for T4 and T3 (8), suggesting that a deranged endocrine function in thyroid neoplasms is due partly to disturbance of the organification of iodine or the coupling process of iodotyrosine (22).…”

Section: Discussionmentioning

confidence: 99%

“…In the thyroid gland, the activity of thyroid peroxidase (TPO), an essential enzyme of endocrine function (15), is known to decrease or to disappear during the course of tumorigenesis (4,24). On the other hand, gamma-glutamyl transpeptidase (GGT) which is detected normally in the rat thyroid gland in the early stage of the fetal period, comes to be expressed in the malignant type of diisopropanolnitrosamine (DIPN) induced-rat thyroid lesions (12,13).…”

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Inverse correlation between thyroid peroxidase(TPO) and gamma-glutamyl transpeptidase(GGT) activities expressed in diisopropanolnitrosamine(DIPN) induced rat thyroid lesions. An enzyme histochemical study.

Yamamoto

Kato

Matsumoto

et al. 1988

Acta Histochem. Cytochem

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The histochemical localization of thyroid peroxidase (TPO) and gammaglutamyl transpeptidase (GGT) in diisopropanolnitrosamine (DIPN) induced rat thyroid lesions was examined.The former was utilized as a marker of the endocrine function of the follicular epithelium and the latter as that of neoplastic growth.The two enzymes revealed an inverse correlation in their patterns of localization, i.e., TPO was positive in most of the benign nodular lesions and negative in malignant nodules, while GGT was positive in the nodules or areas of nodules which were negative for TPO.It is generally accepted that phenotypical alteration occurs in cells and tissues during carcinogenesis of various origins in association with functional retardation and elevated growth activity (2,3,10,18,20,24,25).In the thyroid gland, the activity of thyroid peroxidase (TPO), an essential enzyme of endocrine function (15), is known to decrease or to disappear during the course of tumorigenesis (4, 24). On the other hand, gamma-glutamyl transpeptidase (GGT) which is detected normally in the rat thyroid gland in the early stage of the fetal period, comes to be expressed in the malignant type of diisopropanolnitrosamine (DIPN) induced-rat thyroid lesions (12, 13).The present study was undertaken to investigate the precise correlation in the localization patterns between both enzymes by alternate detection of their activity in serial tissue sections from various stages of carcinogenesis. MATERIALS AND METHODSAnimals Twenty adult male Wistar strain rats of 100-150 g in body weight were injected subcutaneously with DIPN (diisopropanolnitrosamine, Nakarai Chemical Co.) at a dose of 750 mg per kg body weight weekly for 10 weeks. As controls, five rats were prepared and were injected with PBS (phosphate buffered saline) in the same manner. After the final injection, animals were sacrificed weekly by cardiac bleeding, 455

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Inverse correlation between thyroid peroxidase(TPO) and gamma-glutamyl transpeptidase(GGT) activities expressed in diisopropanolnitrosamine(DIPN) induced rat thyroid lesions. An enzyme histochemical study.

Yamamoto

Kato

Matsumoto

et al. 1988

Acta Histochem. Cytochem

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International safety assessment of pesticides: Dithiocarbamate pesticides, ETU, and PTU?A review and update

Vettorazzi¹,

Almeida²,

Burin³

et al. 1996

Teratog. Carcinog. Mutagen.

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International safety assessment of pesticides: Dithiocarbamate pesticides, ETU, and PTU—A review and update

Vettorazzi¹,

Almeida²,

Burin

et al. 1995

Teratog Carcinog Mutagen

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For the last 30 years the Joint FAO/WHO Meeting on Pesticide Residues (JMPR) has carried out toxicological evaluations and safety assessments of dithiocarbamate pesticides, continuously adjusting previous appraisals in the light of new data and advances in the understanding of the principles and mechanisms of toxic action of these compounds. The historical narrative of the evaluative process is followed by an account of the present international safety assessment status of the dithiocarbamate pesticides so far examined by the JMPR. They are ferbam, mancozeb, maneb, metiram, nabam, propineb, thiram, zineb, ziram, and the associated substances, ethylenethiourea (ETU) and propylenethiourea (PTU).

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Cytochemical localization of peroxidase activity in normal, proliferating and neoplastic thyroid tissues of rats. An ultrastructural study

Cited by 6 publications

References 14 publications

Inverse correlation between thyroid peroxidase(TPO) and gamma-glutamyl transpeptidase(GGT) activities expressed in diisopropanolnitrosamine(DIPN) induced rat thyroid lesions. An enzyme histochemical study.

Inverse correlation between thyroid peroxidase(TPO) and gamma-glutamyl transpeptidase(GGT) activities expressed in diisopropanolnitrosamine(DIPN) induced rat thyroid lesions. An enzyme histochemical study.

International safety assessment of pesticides: Dithiocarbamate pesticides, ETU, and PTU?A review and update

International safety assessment of pesticides: Dithiocarbamate pesticides, ETU, and PTU—A review and update

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