Cytochemical Evidence for Varied Dna Complexes in the Nuclei of Undifferentiated Cells

Agrell, Ivar; Bergqvist, Hans-Åke

doi:10.1083/jcb.15.3.604

Cited by 59 publications

(11 citation statements)

References 8 publications

(3 reference statements)

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“…The pattern of Feulgen hydrolysis kinetics differs from one tissue to another in the same organism and undergoes drastic modification during cell differentiation (Agrell & Bergqvist, 1962;Böhm & Sandritter, 1966;Brächet, Hulin & Guermant, 1968). While possibly of significance as regards genetic regulation, the molecular basis of such observations remains ill-understood (Brächet et al 1968), but the obvious relationship of the observed changes of DNA acid lability with the functional state of chromatin warrants the use of this method in comparative studies.…”

Section: Discussionmentioning

confidence: 95%

“…From investigations of sex steroid action in cellproliferation studies (Galand & Leroy, 1971; Martin & Finn, 1971), it appears that different uterine cell types react differently to the same hormonal stimulus. The present paper evaluates genetic regulation by sex steroid action by using methods which enable the chromatin function at the level of individual cells to be studied, namely 'staining' of fixed uterine sections with labelled actinomycin-D followed by radioautography (Brächet & Ficq, 1965) and cytophotometric study of Feulgen reaction kinetics (Agrell & Bergqvist, 1962) on isolated endometrial nuclei. With both methods, different hormonal treatments were found to induce variable patterns of response in rat endometrium epithelial and stromal nuclei.…”

Section: Introductionmentioning

confidence: 99%

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Increased Chromatin Acid Lability and Actinomycin-D Binding in Endometrial Cells Under the Action of Sex Steroids

Leroy¹,

Preumont²,

Galand³

et al. 1972

Journal of Endocrinology

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Modifications of chromatin activity, induced by sex steroids, were studied in rat endometrial tissues by the following semi-quantitative cytochemical methods: 'staining' of fixed uteri by [3H]actinomycin-D followed by radioautography and cytophotometric evaluation of Feulgen reaction kinetics in isolated endometrial nuclei. The following hormonal treatments were applied to groups of animals: oestradiol-17 \g=b\(10\g=m\g);progesterone (2 mg); progesterone (2 mg daily for 4 days) with oestradiol-17\g=b\(0\ m=.\05\g=m\g on the 4th day). With both cytochemical methods, different hormonal treatments were found to induce different patterns of response in endometrial cell types. In luminal epithelium and endometrial stroma, hormonal treatment increased the binding of actinomycin-D in a variable manner as judged from autoradiographic grain counts. Sex steroids also induced an overall increased DNA acid-lability as estimated by cytophotometric evaluation of Feulgen staining intensity. Hormonal treatments induced different hydrolysis curve patterns in both endometrial nuclear types.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Increased Chromatin Acid Lability and Actinomycin-D Binding in Endometrial Cells Under the Action of Sex Steroids

Leroy¹,

Preumont²,

Galand³

et al. 1972

Journal of Endocrinology

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“…However, since the latter cells constitute a very minor proportion of the population in normal brain, it is reasonable to consider most of the larger nuclei as being of neuronal origin. [1][2][3][4][5][6][7][8][9][10][11][12] This increased amount of DNA could be ascribed to a nucleolar satellite of small size which contains DNA and is common in the nuclei of nerve cells .21) However, this nucleolar satellite is conspicuous in the females of many species while in the male it is smaller or absent. Its existence is not a sufficient explanation for our findings since the cerebral tissue of males also has a similar hyperdiploid population.…”

Section: Discussionmentioning

confidence: 99%

Differences in Brain Cell Nuclear DNA as Determined by Flow Cytometry

Hoshino

Wheeler

Nomura

1977

Neurol. Med. Chir.(Tokyo)

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Nuclei selected from various areas of normal brain tissue (rat, canine and man) were isolated by means of tissue grinding, stained with Acriflavin-Feulgen, and the amount of DNA in each individual nucleus was measured. It would appear that two distinct populations of diploid cells exist in the cerebral gray matter, either with regard to their absolute DNA content (approximately 10% more than the diploid amount) or with regard to their ability to be stained by Acriflavin-Feulgen. Microscopic examination of nuclei sorted from each population revealed that those which carried a "hyperdiploid" amount of DNA consisted primarily of nuclei with diameters of 12-16 ƒÊm in contrast to nuclei in the other peak with diameters of 6-8 ƒÊm. These results suggest that some neuronal nuclei in the cerebral gray matter, e.g. pyramidal and Betz cells, may contain a "hyperdiploid" amount of DNA as measured by FCM. The possible biological significance of this observation is not understood at this time.

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“…In this connection, it is also to be considered that Me LEISH ( 1964) and other authors demonstrated that DNA is sometimes Feulgen negative and AGRELL and BERGQUIST ( 1962) showed that, in some materials, there are distinct stain absorption peaks in DNA Feulgen photometry, according to duration of hydrolysis. It could, therefore, be possible that a certain amount of DNA is not stainable with the Feulgen reagent in our material and/ or in the conditions of our DNA determination.…”

mentioning

confidence: 99%