Cyto- and histoarchitectonics of the chicken spleen in the post-vaccination period

Abstract

One of the main functions of the spleen is participation in many immunological processes, protecting the body from various harmful agents. It is a secondary organ of lymphopoiesis and the only organ of immunogenesis, which provides immune control of blood and detects genetically foreign agents in it, and performs the role of a biological filter. Therefore, the Study of its histo-architectonics has theoretical and practical significance. The histostructure of the spleen of chickens has significant differences, particularly the absence of trabeculae, a small amount of connective tissue based around large vessels. The structure of the spleen of one-day-old vaccinated and non-vaccinated chickens is not formed, as there is no differentiation of the white pulp from the red pulp, which is related to the biological features of the chickens' body. According to our research, the presence of lymphoid nodules is observed for the first time in the 25-day age of the vaccinated group chickens. According to morphometric studies, it was established that their number was 2.33 ± 0.42 pcs. Comprehensive farm vaccination programs include ten vaccinations of chickens up to 100 days of age. Under the influence of multiple antigenic stimulations in the following age periods of a 50-day-old bird (after six vaccinations: twice against infectious bronchitis and infectious bursal disease, once against Marek's disease and Newcastle disease), the 75-day (after nine times vaccination), 100-day (after ten times vaccination) number and sizes of lymphoid nodules, as well as periarterial lymphoid sheaths and periellipsoid lymphoid sheaths increased concerning chickens of the control group. Our research established that the histostructure of the spleen of one-day-old chickens of the experimental and control groups is incomplete. The bird's spleen, a peripheral organ of immune protection, is formed at 25 days. However, chickens are vaccinated twice (against Marek's disease and infectious bronchitis) in the incubator when they are less than one day old, and 90% of the planned vaccinations are completed by the age of 75 days. Therefore, studying morphological changes in the spleen of birds of different ages can be an essential tool for re-evaluating preventive treatment protocols in modern poultry farming.